Poli (NM_001136090) Mouse Tagged ORF Clone

CAT#: MR209951

Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 1

Product Images

Circular map for MR209951

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Poli
• Synonyms: Rad30b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209951 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCCAACCCAGAATTAAAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCA
  ACTATGAAGCCAGGAAACTGGGTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCA
  GCTGGTCCTCGTGAACGGAGAAGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTG
  GAGGAATTTAGTCCAGCTGTTGAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGG
  AAAAAAGACTCCAGCAGCTTCCAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAA
  CCAGTCTGTGAACCTACACAACATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATG
  CGGGAAGCCATGTATAATCAGCTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAA
  AGTTAGTGTCTGGGGTTTTTAAACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCAT
  TCACAGTTTGAACCACATAAAGGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTG
  GGAATCAATAGTGTTCATGATCTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAA
  TTGCTCAGCGTATCCAGCAGCTCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACA
  GTCCTTTAGTGAAGAAGATACGTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAA
  TTACTTTCCAGCCTTTTGACCAGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCC
  GTCGGTACTCTGACAAACACTGTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAA
  GTTAGGGACAGGAAATCATGACTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATG
  GTGAACGTGAAGATGCCCTTTCACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCA
  GTGCTAAGAAAGGGCCTATGGACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGC
  CTTTAAAGTGAAGGACACCCACACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCA
  AGTAGAAGAATCGAAAGCACAGGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAG
  ACACAAGTGACTTGCCCCTCCAGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGC
  AGATATTCAAGAAGAAATCCTTTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGT
  CCGCTGCATGCCTCTAGAGGAGTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCC
  CCAGAGATACTGCGCTCCCTAGCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACT
  GAGCCCCGGGAGCACCTCCCATCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAG
  GATGAGCAAACGAGTCAAGGCCCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTT
  CTGGTTTCCATTCCTTTCCTAATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCA
  CAAGCAGACAGCCACCGCCTCTCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGAT
  TCTGCGGAAGAAAAGCTTCCTTTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGG
  TCCAAAAGGAACTGATGGCGGAATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209951 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELL
  EEFSPAVERLGFDENFVDLTEMVEKRLQQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEM
  REAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVL
  GINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKAKIEE
  LLSSLLTRVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNM
  VNVKMPFHLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHTEDSHKEKEANWDCLP
  SRRIESTGTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSC
  PLHASRGVLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLK
  DEQTSQGPTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLESHQGLESRELD
  SAEEKLPFPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136090
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136090.2, NP_001129562.1
• RefSeq Size: 2937 bp
• RefSeq ORF: 2025 bp
• Locus ID: 26447
• UniProt ID: Q6R3M4
• Cytogenetics: 18 E2
• MW: 75.1 kDa
• Gene Summary: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function]