Ppp1r15a (NM_008654) Mouse Untagged Clone

CAT#: MC220161

Ppp1r15a (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp1r15a
• Synonyms: 9630030H21; Gadd34; Myd116
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220161 representing NM_008654
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGAGCCCAAGACCCCAGCATGTCCTGCACTGGAGGGACGCCCACAACTTCTATCTCCTGTCCC
  CACTGATGGGCTTGCTCAGTCGGGCCTGGAGCCGCCTGAGGGGCCCAGAAGTCCCAGAGGCATGGCTGGC
  AAAAACAGTAACAGGAGCAGATCAGATAGAAGCTGCGGCTCTGCTGACACCTACCCCTGTCTCTGGTAAC
  CTCCTCCCTCATGGGGAGACTGAAGAAAGTGGATCTCCTGAACAGAGTCAAGCAGCCCAGAGGCTCTGCC
  TTGTGGAAGCTGAAAGTTCCCCTCCTGAAACTTGGGGACTTTCAAATGTTGATGAGTACAATGCAAAGCC
  AGGACAAGATGACCTTAGAGAGAAGGAAATGGAACGCACAGCTGGCAAGGCCACACTACAGCCCGCTGGC
  CTGCAAGGGGCTGATAAGAGGCTTGGGGAGGTGGTGGCTAGAGAAGAGGGAGTGGCTGAGCCCGCTTATC
  CCACATCACAGCTGGAGGGTGGTCCAGCTGAGAATGAAGAGGATGGAGAAACAGTGAAGACTTACCAAGC
  TTCTGCTGCTTCCATAGCTCCGGGATACAAACCCAGCACCCCTGTGCCTTTCTTGGGGGAGGCAGAACAT
  CAAGCCACGGAAGAAAAAGGAACAGAAAACAAGGCTGACCCCTCCAACTCTCCTTCTTCAGGCTCCCACT
  CCAGAGCCTGGGAGTACTACTCTAGAGAGAAGCCTAAGCAGGAGGGAGAAGCCAAGGTAGAGGCACACAG
  GGCAGGGCAGGGTCACCCTTGTCGGAATGCTGAGGCTGAGGAAGGAGGACCTGAGACAACTTTTGTCTGT
  ACTGGAAATGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAGACAACAGCG
  ATTCGGATTCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCCCATACAAGTGCCTTCCTGAAGGC
  CTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAGACAGCGATTCGGATTCAGCTGAGGAAGACACA
  GCTCAGACCGGTGCCACCCCCCATACAAGTGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACA
  CAGAGGAAGAAAACAGCGATTTGGATTCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCCCATAC
  AAGTGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAAACAGCGATTTGGAT
  TCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCACATACAAGTCCCTTCCTGAAGGCCTGGGTGT
  ATCGCCCAGGAGAGGACACAGAAGATGACACAGAAGAGGAAGAGGACAGTGAGAATGTGGCCCCAGGTGA
  CTCAGAAACAGCTGACTCAAGCCAGAGTCCCTGCCTTCAGCCCCAGCGTTGTCTACCAGGAGAGAAGACC
  AAGGGACGTGGGGAAGAGCCCCCTCTCTTCCAGGTGGCCTTCTATTTACCCGGAGAGAAGCCAGAATCAC
  CTTGGGCTGCACCTAAGCTGCCCCTTCGACTGCAGAGGCGGCTCAGATTGTTCAAAGCCCCCACCCGGGA
  TCAGGACCCCGAGATTCCTCTAAAAGCTCGGAAGGTACACTTCGCTGAGAAAGTCACAGTCCATTTCCTT
  GCTGTCTGGGCAGGACCAGCCCAAGCTGCCCGTCGAGGTCCCTGGGAGCAGTTTGCACGAGATCGAAGCC
  GCTTTGCTCGACGCATTGCCCAGGCAGAGGAGAAGCTGGGTCCCTACCTTACCCCTGATTCCAGGGCCAG
  AGCATGGGCACGCCTTAGAAACCCATCTCTTCCACAGTCCGAGCCTCGCTCTTCCTCTGAGGCCACTCCC
  TTGACCCAAGATGTGACCACACCCTCTCCCCTTCCCAGTGAAACCCCTTCGCCCAGCCTGTACTTGGGAG
  GGAGGCGGGGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008654
• Insert Size: 1974 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008654.2, NP_032680.1
• RefSeq Size: 2345 bp
• RefSeq ORF: 1974 bp
• Locus ID: 17872
• UniProt ID: P17564
• Cytogenetics: 7 B3
• Gene Summary: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. In case of infection with vesicular stomatitis virus (VSV), impairs viral replication.[UniProtKB/Swiss-Prot Function]