Phactr1 (NM_198419) Mouse Untagged Clone

CAT#: MC220117

Phactr1 (untagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phactr1
• Synonyms: 9630030F18Rik; Rpel1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220117 representing NM_198419
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTATCCCAAAATGGATTATTTTCTGGACGTTGAGTCTGCTCACAGACTCTTGGATGTGGAGTCGG
  CGCAGAGGTTCTTCTACAGTCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAAT
  GGCGGCTTCTTCGGAGGATGACATAGACCGGCGGCCCATCAGAAGAGTCCGCTCCAAGAGCGACACGCCA
  TATCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCCGAGGAAGTGGAGAGGCTGGCAGCCATGC
  GTTCCGACTCGCTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACCTGGG
  GAGGATTTTCAAACCCTGGAAATGGAGGAAGAAGAAAAGTGAAAAGTTCAAGCACACGTCAGCAGCTCTG
  GAAAGGAAGATTTCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGAGAGGGGTCTTGAAGGAGATCTACG
  ATAAAGATGGAGAACTCTCTATATCAAATGAAGATGACTCCCTGGAGAACGGACAGTCCCTGAGCTCCAG
  CCAACTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCATGCTCATATGAG
  GTGCTCCAAGCTTCAGACATTATGGATGGACCAGTGTCTGAAGAGAGTCCCTCTGCCAGTGAGTCTGGAG
  TCCTCCTGTCCCAAGATCCTTCAGCCAAACCAGTTCTGTTCCTGCCCCCCAAAAAATCTGCTGCTTTCCC
  TGGAGACCATGAGGAGACCCCAGTGAAACAGTTGTCCCTTCACAAGCAGCCCCCGGCCCTGCCTCCCAAA
  CCCACTGCCCGGATTGCCAACCACTTAACAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGTGA
  AACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGTATGCCTGTAGGGGGGCCAGAGCTCACTCT
  GGCATCCTATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACCATCC
  CAGATGCAGCACCAGCTGCAGTATGGCAGTCACGGCCAGCATCTCCCATCCTCCACCGGCACCTTGCCCA
  TGCACCCCTCAGGCTGCAGGATGATCGACGAGCTGAACAAGACACTCGCCATGACGATGCAGAGGCTGGA
  AAGCTCCGAGCAACGAGTCCCCTGTTCCACGTCTTACCACAGTTCTGGTTTGCACTCAAGCGACGGCATC
  ACAAAAGCAGGACCCATGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGATGACA
  ATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGCCCTGCCTGTATGGCCGGGAGGAGGAGGA
  AGAGGAGGAAGATGAAGATGATGACGCCTCTTTGTACACCAGCTCACTGGCCATGAAGGTGTGCAGGAAG
  GACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCTAAGCGAGAGCTAGAAGAAAAGAACATCCTCCCCA
  GACAGACGGATGAGGAACGGCTGGAGCTCAGACAGCAGATTGGCACCAAGCTCACCAGGCGGCTGAGCCA
  GAGACCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAGCCTCGGAATGAACAAGAAGAACAGGAG
  GAGAAGCGGGAAATCAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGGCCCACAGTGGAAGAACTCCGGG
  AGAGGAAGATCCTTATCCGCTTCAGTGACTACGTGGAAGTGGCAGATGCCCAAGACTATGACCGAAGGGC
  AGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTTTAAA
  AGTACCGAGATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198419
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198419.3, NP_940811.2
• RefSeq Size: 5682 bp
• RefSeq ORF: 1950 bp
• Locus ID: 218194
• Cytogenetics: 13 A4
• Gene Summary: Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization (PubMed:30256902).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).