Fem1b (NM_010193) Mouse Untagged Clone

CAT#: MC219797

Fem1b (untagged) - Mouse feminization 1 homolog b (C. elegans) (Fem1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fem1b
• Synonyms: mKIAA0396
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219797 representing NM_010193
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCCTGGCTGGCTATGTGTACAAGGCGGCCAGCGAGGGCAAGGTGCTCACTCTGGCTGCCTTGC
  TCCTTAACCGGTCAGAGAGCGATATCCGCTACCTGCTGGGCTATGTCAGTCAGCAGGGAGGACAGCGCTC
  CACACCCCTCATCATCGCAGCCCGCAATGGGCACGCCAAGGTGGTGCGCTTGCTGTTAGAACACTACCGT
  GTGCAGACCCAGCAGACCGGCACCGTCCGCTTCGACGGGTATGTCATTGACGGTGCCACTGCTCTTTGGT
  GTGCTGCGGGAGCCGGACATTTTGAAGTTGTTAAACTTCTAGTCAGCCATGGAGCCAACGTGAACCACAC
  CACAGTCACTAACTCAACTCCATTGCGGGCAGCATGCTTTGATGGCAGACTGGACATTGTGAAATATTTG
  GTTGAAAATAATGCCAATATCAGCATTGCCAACAAGTATGACAACACCTGCCTAATGATCGCAGCATATA
  AAGGGCACACTGATGTGGTCAGATACCTCTTAGAACAACGTGCTGATCCCAATGCTAAAGCACACTGTGG
  AGCCACAGCTTTGCACTTTGCAGCCGAAGCTGGTCACATTGACATTGTGAAAGAACTGATAAAATGGAGA
  GCTGCAATAGTGGTGAATGGCCATGGGATGACACCATTGAAGGTGGCCGCTGAAAGCTGTAAAGCTGATG
  TCGTTGAACTGTTGCTCTCTCATGCTGATTGTGACCGCAGAAGTCGGATTGAAGCCTTGGAGCTCTTGGG
  TGCCTCCTTTGCAAATGATCGTGAGAACTATGACATCATGAAGACATACCACTATTTATATTTAGCTATG
  TTGGAGAGATTTCAGGATGGTGACAACATTCTTGAAAAAGAGGTTCTCCCACCCATCCATGCTTATGGGA
  ACAGAACTGAGTGTAGGAACCCACAGGAATTGGAGGCTATTCGGCAAGACAGAGATGCTCTTCACATGGA
  GGGCCTTATAGTTCGGGAACGGATTTTAGGTGCTGACAACATTGATGTTTCCCACCCCATCATTTACAGA
  GGGGCTGTCTATGCTGATAACATGGAGTTCGAGCAGTGCATCAAATTGTGGCTCCACGCACTACACCTGA
  GGCAGAAAGGTAACAGGAATACCCACAAAGATCTGCTTCGGTTTGCTCAAGTCTTCTCTCAGATGATACA
  CCTCAATGAAGCTGTGAAGGCCCCAGACATAGAGTGCGTTTTGAGATGCAGTGTCTTGGAAATAGAGCAG
  AGCATGAACAGAGTTAAAAATATCTCCGATGCCGACGTCCACAGTGCCATGGATAACTATGAGTGTAACC
  TCTATACCTTTCTGTACCTCGTGTGCATCTCCACCAAGACACAGTGTAGCGAAGAAGATCAGTGCAGAAT
  TAACAAGCAGATCTACAACCTGATTCACCTGGACCCCAGAACACGGGAAGGTTTTTCCTTGCTACACCTG
  GCTGTCAACTCGAACACACCAGTTGATGATTTCCATACTAACGATGTCTGCAGCTTTCCCAACGCTCTGG
  TCACAAAGCTCCTGCTGGACTGTGGCGCTGAGGTAAATGCAGTGGACAATGAGGGGAACAGTGCCCTCCA
  CATTATCGTCCAGTACAACAGGCCCATCAGTGATTTTCTGACCTTGCACTCCATCATCATCAGCCTTGTG
  GAGGCTGGAGCTCACACCGACATGACAAACAAGCAGAATAAAACTCCGCTAGACAAAAGTACAACTGGGG
  TGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCCTCAAGTGCCTGGCTGCCCGAGCAGTTCGGGC
  TAATGACATTAACTACCAAGACCAGATCCCCCGGACTCTTGAAGAGTTTGTTGGATTTCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010193
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010193.4, NP_034323.1
• RefSeq Size: 6505 bp
• RefSeq ORF: 1884 bp
• Locus ID: 14155
• UniProt ID: Q9Z2G0
• Cytogenetics: 9 33.87 cM
• Gene Summary: Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1 (By similarity).[UniProtKB/Swiss-Prot Function]