Samhd1 (NM_018851) Mouse Untagged Clone

CAT#: MC218733

Samhd1 (untagged) - Mouse SAM domain and HD domain, 1 (Samhd1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Samhd1
• Synonyms: E330031J07Rik; Mg11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218733 representing NM_018851
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCACTTTTGGGGTGTGGTGTCAGTGCGGCTGCGCGCGAGCCGGTGCCGCGGTACTTAACTTCGC
  AGCCCCGGGTGTCGGAGGTAGCCATGCAGAGCGCACCCTTGGAGCAGCCAGCTAAGCGACCCCGCTGCGA
  TGGCAGCCCAAGGACGCCACCGAGCACTCCTCCTGCAACAGCTAATCTGTCTGCAGACGACGACTTCCAA
  AACACCGACCTCCGAACCTGGGAACCGGAGGACGTGTGCTCCTTCTTAGAGAATCGTGGTTTCCGAGAGA
  AGAAAGTGCTGGACATCTTCAGAGACAATAAAATCGCCGGCTCGTTTCTGCCCTTTTTGGATGAGGATCG
  TCTGGAAGATCTGGGAGTAAGTTCCTTGGAGGAGAGGAAGAAGATGATAGAATGTATCCAGCAGCTGAGT
  CAGTCTCGGATTGATCTAATGAAGGTATTTAATGATCCCATTCATGGCCACATTGAGTTCCACCCTCTCC
  TTATCAGAATCATCGACACACCTCAGTTCCAGCGACTTCGCTATATCAAGCAGCTGGGGGGCGGCTACTA
  TGTTTTCCCTGGAGCGTCCCACAATCGCTTCGAACACAGTCTCGGAGTGGGGTACCTAGCAGGCTGCCTG
  GTGCGAGCACTTGCCGAAAAACAGCCAGAGCTACAGATCAGTGAGCGAGATATACTCTGTGTTCAGATTG
  CGGGGCTCTGCCACGACCTAGGTCATGGGCCATTTTCTCATATGTTTGATGGAAGGTTTATCCCACGGGC
  TCGCCCAGAGAAAAAGTGGAAGCACGAACAGGGCTCCATTGAGATGTTTGAGCATCTGGTCAATTCTAAT
  GAACTCAAACTTGTCATGAAGAACTATGGTCTCGTCCCTGAAGAAGACATTACCTTTATCAAGGAACAAA
  TTATGGGACCACCTATAACACCAGTCAAAGATTCCTTGTGGCCGTATAAAGGCCGCCCTGCCACGAAGAG
  CTTCCTTTACGAGATAGTGTCTAACAAGAGGAATGGCATCGACGTAGACAAATGGGATTATTTTGCCAGA
  GACTGTCACCATCTTGGAATCCAAAATAATTTTGATTACAAGCGCTTCATTAAGTTTGCCCGTATCTGTG
  AAGTGGAGTACAAGGTCAAGGAGGACAAGACCTACATCCGTAAGGTGAAGCACATTTGTTCGAGAGAAAA
  GGAGGTTGGAAATCTGTATGACATGTTCCACACTCGCAACTGCTTACACCGAAGAGCTTACCAACACAAG
  ATCAGCAACCTCATCGACATAATGATTACAGATGCCTTCCTCAAAGCAGACCCCTACGTGGAGATTACAG
  GGACTGCCGGGAAGAAGTTCCGCATTTCCACAGCCATTGATGACATGGAAGCCTTCACTAAGTTGACGGA
  TAACATTTTTCTGGAGGTTTTACACTCTACTGATCCACAGTTGTCTGAGGCCCAAAGTATTTTAAGGAAC
  ATTGAATGCCGCAATCTATACAAGTATTTGGGTGAGACCCAGCCAAAGCGTGAGAAGATTAGGAAGGAAG
  AATATGAACGGCTTCCCCAAGAAGTTGCTAAAGCCAAACCTGAAAAAGCCCCGGATGTTGAACTGAAGGC
  CGAAGATTTCATAGTTGATGTTATCAATGTGGATTATGGGATGGAAGACAAGAATCCAATCGATCGTGTT
  CACTTCTATTGTAAGAGCAACAGCAAGCAAGCGGTCAGGATCAATAAAGAGCAGGTGTCACAACTGCTGC
  CAGAGAAATTTGCAGAGCAGCTCATTCGGGTGTACTGTAAGAAGAAAGACGGGAAGAGCCTGGACGCCGC
  CGGGAAGCACTTTGTTCAGTGGTGTGCGCTCAGGGACTTCACCAAGCCACAGGATGGTGACATTATAGCT
  CCACTCATAACACCTCTGAAATGGAATAATAAGACTTCATCTTGCCTCCAAGAAGTATCCAAAGTAAAAA
  CATGTCTAAAATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_018851
• Insert Size: 1977 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018851.3, NP_061339.3
• RefSeq Size: 3887 bp
• RefSeq ORF: 1977 bp
• Locus ID: 56045
• UniProt ID: Q60710
• Cytogenetics: 2 H1
• Gene Summary: Isoform 1: Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (By similarity). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed:23972988, PubMed:23872947, PubMed:26667483, PubMed:29379009). Likewise, suppresses LINE-1 retrotransposon activity (PubMed:26667483). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (By similarity). Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (By similarity). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (By similarity). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (By similarity). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (By similarity). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (PubMed:29669924).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).