Kctd8 (NM_175519) Mouse Untagged Clone

CAT#: MC216567

Kctd8 (untagged) - Mouse potassium channel tetramerisation domain containing 8 (Kctd8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kctd8
• Synonyms: A730087N02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216567 representing NM_175519
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAAGGACACGGGCAGCGGCGGCAGCACCATCCTCCCGATCAGCGAGATGGTGTCCGCGTCCA
  GCTCTCCCGGCGCTCCGCTGGCCGCCGCCCCGGGGCCCTGCGCCCCGTCGCCCTTCCCCGAGGTAGTGGA
  GCTGAATGTTGGCGGCCAGGTTTATGTGACCAAGCATTCGACGTTACTCAGCGTCCCGGACAGCACTCTG
  GCCAGCATGTTCTCACCCTCTAGTCCCCGGGGCGGCGCTAGGCGCCGGGGCGACTTGCCCAGGGACAGCC
  GCGCGCGCTTCTTCATCGACCGCGACGGCTTCCTCTTTAGGTACGTGCTGGATTACCTGCGCGACAAGCA
  GCTGGCACTGCCCGAGCACTTTCCCGAGAAGGAGAGGCTCCTGCGCGAAGCAGAGTTCTTTCAGCTCACC
  GACCTGGTCAAGCTGCTGTCGCCCAAGGTCACCAAGCAGAACTCGCTCAACGATGAGTGCTGCCAGAGCG
  ACCTGGAGGACAACGTTTCCCAGGGCAGCAGCGACGCACTGCTGCTGCGTGGGGCGGCGGCTGGCGCGCC
  CTCGGGCTCTGGGGCACATGGTGTCAGTGGTGTAGTCGGTGGTGGCAGCGCTCCGGACAAGCGCTCTGGG
  TTCCTCACCCTGGGCTACCGTGGCTCTTACACCACGGTGCGAGATAACCAGGCAGATGCCAAGTTCAGGC
  GTGTGGCGCGCATCATGGTGTGTGGGCGCATAGCCTTGGCCAAGGAGGTCTTTGGGGACACTCTTAATGA
  GAGTCGCGACCCTGACCGTCAGCCTGAGAAGTACACATCCCGCTTCTACCTCAAGTTCACCTACTTGGAG
  CAGGCGTTCGATCGACTGTCTGAGGCCGGCTTCCACATGGTGGCGTGCAACTCCTCTGGCACTGCCGCCT
  TTGTCAACCAGTACCGAGACGACAAGATCTGGAGCAGTTACACTGAATACATCTTCTTCCGACCACCTCA
  GAAAATAGTGTCACCCAAGCAAGAACATGAAGACAGGAAACGCGACAAAGTCACAGACAAAGGAAGTGAG
  AGTGGGACTTCCTGCAATGAGCTCTCCACATCCAGCTGTGACAGCCACTCAGAGGCCAGCACTCCACAGG
  ACAACCCAGCCAACACTCAGCAGGCTGCAGCTCACCAGCCTAACACCTTAACCTTGGATAGACCCTCCAG
  GAAAGCACCTGTTCAGTGGATGCCACCACCAGACAAGCGCAGAAACAGTGAACTCTTTCAGTCACTCATC
  AGCAAGTCCCGAGAAACAAATCTCTCCAAAAAGAAGGTCTGTGAGAAGCTAAGTGTAGAAGAAGAAATGA
  AAAAGTGTATTCAGGATTTTAAAAAAATCCATATTCCAGATTGTTTTCCAGAGCGCAAACGCCAGTGGCA
  ATCTGAACTCCTCCAAAAATATGGGTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175519
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175519.5, NP_780728.3
• RefSeq Size: 2889 bp
• RefSeq ORF: 1431 bp
• Locus ID: 243043
• UniProt ID: Q50H33
• Cytogenetics: 5 C3.1
• Gene Summary: Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.[UniProtKB/Swiss-Prot Function]