Dbnl (NM_001146308) Mouse Untagged Clone

CAT#: MC215710

Dbnl (untagged) - Mouse drebrin-like (Dbnl), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dbnl
• Synonyms: Abp1; mAbp1; SH3P7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215710 representing NM_001146308
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGAACCTGAGCCGGAACGGGCCGGCGCTGCAGGAGGCCTACGTGCGCGTAGTCACCGAGAAAT
  CCCCGACCGACTGGGCTCTTTTTACCTATGAAGGCAACAGCAATGACATCCGTGTGGCTGGCACAGGAGA
  GGGAGGCCTGGAGGAGCTGGTGGAAGAGCTCAACAGCGGGAAGGTGATGTACGCCTTCTGCAGGGTGAAG
  GACCCCAACTCCGGCCTGCCCAAGTTTGTCCTCATCAACTGGACAGGAGAGGGTGTGAATGATGTGCGGA
  AAGGAGCATGTGCCAACCACGTCAGCACCATGGCCAACTTCCTGAAGGGTGCCCACGTGACCATCAATGC
  CCGGGCCGAGGAGGATGTGGAGCCTGAGTGCATCATGGAGAAGGTTGCCAAGGCCTCTGGGGCCAACTAC
  AGCTTCCATAAGGAAAGCACCTCCTTCCAGGATGTAGGGCCGCAGGCCCCAGTGGGCTCTGTGTACCAGA
  AGACCAATGCCATATCTGAGATCAAGAGAGTCGGCAAGGATAACTTCTGGGCCAAAGCTGAGAAGGAAGA
  AGAGAACCGCCGCCTGGAGGAGAAGCGGCGTGCCGAAGAGGAGCGGCAGCGGTTGGAGGAGGAGCGACGA
  GAGCGGGAGCTGCAGGAGGCTGCCCGACGTGAGCAGCGCTACCAGGAACAGCACAGATCAGCTGGAGCCC
  CGAGCCCTTCCAGCAGGACAGGTGAGCCAGAGCAGGAAGCCGTTTCAAGGACCAGACAGGAGTGGGAGTC
  TGCTGGGCAGCAGGCCCCACACCCACGAGAGATTTTCAAGCAGAAGGAAAGGGCAATGTCCACCACCTCT
  GTCACCAGCTCGCAGCCGGGCAAGCTGAGGAGCCCCTTCCTGCAGAAGCAACTCACTCAACCAGAAACCT
  CCTACGGCCGAGAGCCCACAGCTCCTGTCTCCCGGCCTGCAGCAGGTGTCTGTGAGGAGCCAGCGCCTAG
  CACTCTGTCTTCTGCCCAGACAGAAGAAGAACCTACATATGAAGTACCCCCAGAGCAGGACACCCTCTAT
  GAGGAACCACCACTGGTACAGCAGCAAGGGGCTGGCTCCGAACACATTGACAACTACATGCAGAGCCAGG
  GCTTCAGTGGACAAGGGCTGTGCGCCCGGGCCTTGTATGACTACCAGGCAGCTGATGACACCGAGATCTC
  CTTTGACCCTGAGAACCTAATCACAGGCATCGAGGTGATTGACGAAGGCTGGTGGCGAGGCTATGGGCCT
  GACGGCCACTTTGGCATGTTTCCTGCCAACTACGTGGAGCTCATAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146308
• Insert Size: 1311 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146308.1, NP_001139780.1
• RefSeq Size: 2369 bp
• RefSeq ORF: 1311 bp
• Locus ID: 13169
• UniProt ID: Q62418
• Cytogenetics: 11 3.87 cM
• Gene Summary: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).