Hsd3b7 (NM_133943) Mouse Untagged Clone

CAT#: MC212072

Hsd3b7 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsd3b7
• Synonyms: AI195443; BB098564
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212072 representing NM_133943
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACTCTGCACAGGTGCCAACACTGGTATACCTGGTCACAGGTGGCTGCGGCTTCCTGGGGGAAC
  ATATTGTTCGGATGCTGCTGGAACGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG
  TTCTTGGCTGGAGGAGCTGAAAGCAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC
  CATGAGGTGGCAGCAGCCATGTCTGGATCACATGTGGTCATCCATACAGCTGGGTTGGTGGATGTGTTTG
  GGAAGGCCAGTCCAAAGACCATCCACAAAGTCAACGTGCAGGGCACACAGAATGTGATTGATGCTTGTGT
  GCAGACTGGCACTCAGTACCTGGTCTACACGAGCAGCATGGAAGTGGTGGGGCCTAACATCAAGGGCCAC
  CCCTTCTACAGGGGCAATGAAGATACCCCATATGAGGCAGTCCACAGCCATCCCTACCCATGCAGTAAAG
  CCCTTGCTGAGCAGCTGGTCCTCGAGGCCAATGGAAGGAAGGTCAATGGAGGGCTACCCCTGGTGACATG
  TGCCCTTCGACCCACGGGCATTTATGGTGAAGGTCATCAGGTCATGAGAGACTTCTACTACCAGGGACTG
  CGCTTTGGAGGTCGTCTATTTCGGGCCGTCCCAGCTTCTGTGGAGCACGGTCGGGTCTATGTTGGCAATG
  TTGCTTGGATGCACATACTGGTGGCCCGGGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTA
  TTTCTGCTATGATAAGTCACCTTATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGT
  CTTCGACTGATAGGCGCCCACCCACTGCTGCCCTACTGGCTGCTAGTGCTGCTGGCTACCCTCAATGCCC
  TGCTGCAGTGGCTGCTCCGCCCACTGGTGCTGTACACACCCCTGCTGAATCCCTACACGCTGGCTATGGC
  CAACACCACCTTTACTGTCAGTACCAACAAGGCACAGCGGCATTTTGGCTACAAGCCCCTCTTCTCATGG
  GAAGAGAGCAGGACCCGCACCATTCAGTGGGTGCAGGCGATGGAGGGTTCAGCTCGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133943
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133943.2, NP_598704.2
• RefSeq Size: 1823 bp
• RefSeq ORF: 1110 bp
• Locus ID: 101502
• UniProt ID: Q9EQC1
• Cytogenetics: 7 F3
• Gene Summary: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).[UniProtKB/Swiss-Prot Function]