Hsd3b7 (NM_133943) Mouse Untagged Clone

SKU MC212072

Hsd3b7 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Hsd3b7
• Synonyms: AI195443; BB098564
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212072 representing NM_133943; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGACTCTGCACAGGTGCCAACACTGGTATACCTGGTCACAGGTGGCTGCGGCTTCCTGGGGGAAC; ATATTGTTCGGATGCTGCTGGAACGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG; TTCTTGGCTGGAGGAGCTGAAAGCAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC; CATGAGGTGGCAGCAGCCATGTCTGGATCACATGTGGTCATCCATACAGCTGGGTTGGTGGATGTGTTTG; GGAAGGCCAGTCCAAAGACCATCCACAAAGTCAACGTGCAGGGCACACAGAATGTGATTGATGCTTGTGT; GCAGACTGGCACTCAGTACCTGGTCTACACGAGCAGCATGGAAGTGGTGGGGCCTAACATCAAGGGCCAC; CCCTTCTACAGGGGCAATGAAGATACCCCATATGAGGCAGTCCACAGCCATCCCTACCCATGCAGTAAAG; CCCTTGCTGAGCAGCTGGTCCTCGAGGCCAATGGAAGGAAGGTCAATGGAGGGCTACCCCTGGTGACATG; TGCCCTTCGACCCACGGGCATTTATGGTGAAGGTCATCAGGTCATGAGAGACTTCTACTACCAGGGACTG; CGCTTTGGAGGTCGTCTATTTCGGGCCGTCCCAGCTTCTGTGGAGCACGGTCGGGTCTATGTTGGCAATG; TTGCTTGGATGCACATACTGGTGGCCCGGGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTA; TTTCTGCTATGATAAGTCACCTTATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGT; CTTCGACTGATAGGCGCCCACCCACTGCTGCCCTACTGGCTGCTAGTGCTGCTGGCTACCCTCAATGCCC; TGCTGCAGTGGCTGCTCCGCCCACTGGTGCTGTACACACCCCTGCTGAATCCCTACACGCTGGCTATGGC; CAACACCACCTTTACTGTCAGTACCAACAAGGCACAGCGGCATTTTGGCTACAAGCCCCTCTTCTCATGG; GAAGAGAGCAGGACCCGCACCATTCAGTGGGTGCAGGCGATGGAGGGTTCAGCTCGGTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133943
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133943.2, NP_598704.2
• RefSeq Size: 1823 bp
• RefSeq ORF: 1110 bp
• Locus ID: 101502
• UniProt ID: Q9EQC1
• Cytogenetics: 7 F3
• Summary: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).[UniProtKB/Swiss-Prot Function]