Rap2a (NM_029519) Mouse Untagged Clone

CAT#: MC211707

Rap2a (untagged) - Mouse RAS related protein 2a (Rap2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rap2a
• Synonyms: 5830461H18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211707 representing NM_029519
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGAGTACAAAGTGGTGGTGCTGGGCTCGGGCGGGGTAGGCAAATCCGCCCTGACCGTGCAGTTCG
  TGACCGGCACCTTCATTGAGAAATACGACCCCACCATCGAGGACTTCTACCGCAAGGAGATCGAGGTGGA
  CTCCTCGCCTTCGGTGCTGGAGATCCTGGACACCGCGGGCACCGAGCAGTTCGCGTCCATGCGGGACCTG
  TACATCAAGAACGGCCAGGGCTTCATCCTGGTCTACAGCTTGGTCAACCAGCAGAGCTTCCAAGACATCA
  AGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTATGAGAAGGTTCCAGTCATCCTGGTCGGGAACAA
  AGTGGACCTGGAGAGTGAGAGAGAAGTGTCCTCCAACGAAGGCCGCGCCTTGGCCGAGGAGTGGGGCTGC
  CCCTTTATGGAGACTTCTGCTAAGAGCAAAACAATGGTGGATGAGCTCTTTGCAGAAATTGTGCGGCAGA
  TGAACTATGCAGCTCAGCCTGACAAAGATGACCCATGCTGTTCTGCCTGTAACATACAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029519
• Insert Size: 552 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029519.3, NP_083795.2
• RefSeq Size: 4174 bp
• RefSeq ORF: 552 bp
• Locus ID: 76108
• UniProt ID: Q80ZJ1
• Cytogenetics: 14 64.72 cM
• Gene Summary: Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.[UniProtKB/Swiss-Prot Function]