Trmu (NM_028063) Mouse Untagged Clone

CAT#: MC211275

Trmu (untagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trmu
• Synonyms: 1110005N20Rik; 1600025P05Rik; AI314320; Trmt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211275 representing NM_028063
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGCTGCGGCACGTCGTGTGCGCCCTGTCTGGCGGTGTGGACAGCGCGGTGGCGGCGCTGCTGT
  TGCGGCGGAGAGGTTACCAGGTGACAGGGGTATTTATGAAGAACTGGGACTCGCTGGATGAGCAGGGCGT
  TTGTGCTGCTGACAAGGACTGTGAAGATGCGTATAAAGTTTGTCAGATCTTAGACATCCCTTTCCACCAG
  GTGTCCTATGTGAAGGAGTATTGGAATGATGTGTTCAGTGACTTTTTGAACGAGTATGAGAAAGGAAGGA
  CTCCAAACCCCGACATCAACTGCAATAAGCATATCAAGTTCAGTTGCTTTTATCACTATGCTGTGGACAA
  TCTTGGAGCAGATGCCGTTGCCACTGGCCACTACGCCAGGACCTCACTGGAGGATGAGGAAGTCTTTGAG
  CAAAAGCACACTAAGAAGCCTGACGGGCTGTTCAGAAATCGATTTGAAGTCAGAAACCCCGTAAAACTTC
  TCCAAGCAGCTGACAGCTTTAAAGACCAGACCTTCTTTCTCAGCCAGGTTTCCCAGGATGCCCTGAGAAG
  GACCATCTTTCCCCTGGGGGAACTAACAAAAGATTTTGTAAAGAAAATAGCCGCGGAGAATAGCCTCCAT
  CACGTGCTTCAGAAGAGAGAGAGCATGGGCATCTGCTTCATTGGTAAGAGAAACTTGGAGCATTTCCTTC
  TTCAGTATTTGCAGCCTCGGCCTGGAAAGTTTGTTTCTATCGAGGATAATACAGTTCTGGGAACACACAA
  AGGTTGGTTCCTGTACACTTTGGGCCAGAGAGCTAAGATCAGCGGCTTGAGAGAGCCCTGGTACGTGGTG
  GAGAAGGATGGCACCAAGGGTGACGTGCTTGTGGCTCCCCGGGTGGACCACCCAGCTCTGTACAGGGACC
  TGCTCAGGACCAACCGAGTACACTGGATTGCGGAGGAGCCACCTGCTGCCTTGGTCAGAGACAAGATGAT
  GGAGTGCCATTTCCGATTCCGCCACCAGATGGCACTAGTGCCCTGCGTGCTGACTCTCAATCAGGATGGC
  ACTGTGTGGGTAACGGCTGTGAAGGCTGTGAGGGGCCTTGCCCTGGGACAGTTTGCAGTGTTCTACAAGG
  GTGAGGAGTGCCTCGGCAGTGGGAAAATCCTGCGGCTAGGGCCGTCTGCCTACACACTCCAGAAGGGTAA
  GAACAGAACTCGAGTGGCTCCCGAGGCCTCCAGTGACAGCCCAGGCCTGCACCCCACATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028063
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028063.2, NP_082339.1
• RefSeq Size: 1520 bp
• RefSeq ORF: 1254 bp
• Locus ID: 72026
• UniProt ID: Q9DAT5
• Cytogenetics: 15 E2
• Gene Summary: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.[UniProtKB/Swiss-Prot Function]