Blzf1 (NM_025505) Mouse Untagged Clone

CAT#: MC210340

Blzf1 (untagged) - Mouse basic leucine zipper nuclear factor 1 (Blzf1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Blzf1
• Synonyms: 1700030G05Rik; Blzf1l; Golgin-45; Jem-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210340 representing NM_025505
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAATGACTACCCTTAAAAGTTCGGAAAACAAAGGGAAACAAGAAATGATGAAAGGCATCCTTA
  CTTCGACCCCAATCCGAGGAGCAGGAGATGGAATGGAAACGGAGGAGCCCCCTAAGTCTGTTGAAGTCAC
  CCACGGAGTCCAACCCATAAATCAACATGTCCTTCCAAGTCCACGGAAGAAAGTGTCCTCAGACAGCCCG
  GGCGTTCTTCAGCTGGGGAAGATTCTTAATGAAAGAACAGTGGAAGTTGAAGCTGTCAGAATATTTGTTC
  CCAAAGCTGCTATCACTCACGACATCCCCACCAAGAACACAAAGGTTAAGTCCCTAGGACATCACAGAGA
  AGAACTCCACAATCAGGCAGAAGTGGTCACAGACCCAAGGAAGGAACTCTCAGAGGTAAAGAAGGTTTTG
  GAAAAGCTCAAGAATTCTGAAAGGAGGCTACTTCAGGACAAAGAAGGCCTTTCCAATCAGCTCCGAGTAC
  AGACAGAGATAAATCGTGAGTTAAAGAAGTTACTCGTGGCTTCTGTTGGGGATGACCCGCAGTATCACTT
  TGAACGTCTAGCCCGGGAGAAGAACCAGCTCATCTTAGAAAATGAAGCACTAGGTCGGAACACAGCCCAG
  CTTTCTGAACAGCTGGAACGGATGTCAATACAGTGCGATGTCTGGAGAAGTAAATTCCTTGCAAGCAGGG
  TAATGGCAGATGAGTTAACAAACTTCAGAGTGGTTTTACAGCGTCAGAACCGAGATGCCCAGAGCGCTAT
  CCAGGATCTCCTGAGTGAACGAGAGCAGTTTCGTCAGGAAATGACCTCTACCCAAAAATTTTTGGAAGAA
  CTCTTGGTCTCCTTACAGTGGGGGAGAGAGCAGACATACTCCCCTAACACACAGCCCCATAGCACGGCAG
  ACTTAGCACTCACGAACCATGGACTGGCACAAGCAATACATGCCCATCTGTTGGGGAATGTTGGCATTAG
  CCATCAAAAGAAGATTCCAACAACAGTCGAATTCTGCAGTACCCCAGCTGAGAAAATGGCTGAAAAGGTT
  CTGCGGATTTTGGATCCAGTTGCCTGTACAGAGAGCTCTCCGGACAACCAATTTGCTGAATCTTCACCAA
  CCACATTACTCACTACAAAGAAAAATATTGGACGTTTCCATCCCTATACCAGATATGAAAATATAACTTT
  CAATTGCTGCAATCACTGCCAGGGGGAGCTCATTGCTCTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025505
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025505.4, NP_079781.2
• RefSeq Size: 3948 bp
• RefSeq ORF: 1233 bp
• Locus ID: 66352
• UniProt ID: Q8R2X8
• Cytogenetics: 1 H2.2
• Gene Summary: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).