Chst5 (NM_019950) Mouse Untagged Clone

CAT#: MC210095

Chst5 (untagged) - Mouse carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 (Chst5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chst5
• Synonyms: AI173964; Gn6st-3; GST-4; I-GlcNAc-6-ST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210095 representing NM_019950
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTACCCCGTTTCTCCAGCACTGTCATGCTTTCGCTCCTGATGGTACAGACTGGCATCCTGGTCT
  TCCTGGTCTCCCGGCAAGTGCCATCGTCCCCAGCAGGCCTTGGGGAGCGTGTGCACGTGCTGGTACTGTC
  CTCGTGGCGCTCGGGCTCGTCCTTCGTGGGCCAGCTCTTCAGCCAACACCCCGATGTCTTCTACCTGATG
  GAGCCGGCTTGGCACGTCTGGGATACGTTGTCGCAGGGCAGTGCCCCCGCACTCCACATGGCCGTGCGTG
  ACCTGATCCGCTCAGTGTTCCTATGCGACATGGACGTATTTGATGCCTACCTGCCCTGGCGCCGCAACAT
  CTCGGATCTCTTCCAGTGGGCGGTGAGCCGCGCATTGTGCTCACCTCCGGTCTGCGAAGCCTTCGCTCGT
  GGCAACATCAGCAGCGAGGAGGTGTGTAAGCCTCTGTGCGCAACGCGGCCCTTCGGCCTGGCTCAGGAAG
  CCTGCAGCTCCTATAGTCACGTCGTGCTCAAGGAGGTGCGCTTCTTTAACCTACAGGTGCTCTACCCGCT
  GCTCAGCGACCCTGCGCTCAACCTGCGCATCGTGCACCTAGTGCGCGACCCGCGGGCCGTGCTGCGCTCC
  CGAGAGCAGACAGCCAAGGCGCTGGCACGGGACAATGGCATCGTCCTGGGTACCAACGGCACGTGGGTGG
  AGGCGGACCCCCGGCTGCGCGTGGTCAACGAGGTATGCCGCAGCCATGTGCGCATCGCAGAGGCAGCCTT
  GCACAAGCCGCCGCCCTTCTTGCAAGATCGCTACCGCCTGGTGCGCTACGAGGATCTGGCCCGGGACCCA
  CTCACCGTAATCCGTGAACTCTATGCCTTCACCGGCCTGGGTCTCACGCCGCAGCTCCAGACTTGGATCC
  ACAATATCACGCATGGTTCAGGGCCAGGCGCGCGCCGTGAAGCCTTCAAGACCACATCCAGGGATGCGCT
  CAGTGTATCCCAGGCCTGGCGCCACACGCTGCCCTTTGCCAAGATTCGCCGGGTCCAGGAACTGTGCGGG
  GGTGCACTGCAGCTGCTGGGTTACCGGTCTGTGCATTCGGAGCTTGAGCAAAGGGACCTCTCTCTGGACC
  TCCTGCTGCCAAGAGGCATGGACAGTTTCAAGTGGGCATCGTCCACGGAGAAGCAACCGGAATCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019950
• Insert Size: 1188 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019950.2, NP_064334.1
• RefSeq Size: 1935 bp
• RefSeq ORF: 1188 bp
• Locus ID: 56773
• UniProt ID: Q9QUP4
• Cytogenetics: 8 E1
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors.[UniProtKB/Swiss-Prot Function]