Ldlrad4 (NM_172631) Mouse Untagged Clone

CAT#: MC209900

Ldlrad4 (untagged) - Mouse DNA segment, Chr 18, ERATO Doi 653, expressed (D18Ertd653e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ldlrad4
• Synonyms: 8230401C20Rik; A430083H02; A430108L08Rik; C18orf1; D18Ertd653e; D330030L18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209900 representing NM_172631
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCGTTCACAGAGTGCAAATTCACCTGCACCAGCGGTAAAT
  GCTTGTATCTTGGTTCTCTGGTCTGTAACCAACAGAACGACTGTGGGGACAACAGTGACGAAGAGAACTG
  CCTCCTGGTGACCGAGCACCCACCGCCGGGCATCTTCAACTCCGAGCTGGAATTCGCGCAGATCCTTATC
  ATTGTTGTGGTGGTGACGGTGATGGTGGTGGTCGTTGTCTGCCTACTGAACCACTACAAAGTCTCCACAC
  GCTCCTTCATCAACCGCCCCAACCAGAGCCAGAGACAGGAGGACGGGCTGCAGCCGGAAGGATCCCTGTG
  GCCTTCTGATAGCTCCGTGCAGCGCCCAGGGGCTTCAGAGATCATGTGTGCCCCACGGGGCAGGGATAGG
  TTTACTACCCCATCTTTCATCCAGAGGGATCCATTCAGTCGTTTCCAGCCCACCTACCCCTATGTGCAGC
  ACGAGATTGACTTGCCTCCCACCATCTCCCTGTCAGACGGGGAGGAGCCGCCTCCTTACCAAGGACCCTG
  CACGCTACAGCTCCGGGACCCAGAGCAGCAGATGGAACTCAACCGAGAGTCCGTGAGGGCCCCGCCCAAT
  CGAACCGTTTTTGACAGTGACTTGATAGACATTTCTATGTACAACGGGGGACCATGCCCACCAAGCAGCC
  ACTCGGGCATCAGCGCAGCTACCTGTAGCAGTAACGGAAGAATGGAGGGGCCACCCCCGACCTACAGCGA
  GGTGATGGGCCACTACCCAGGCACCTCGTTCTTCCATCACCAGCACAGCAACACACACAGGGGCAGCAGA
  CCACAGTTTCAGCCGAACAACTCAGAGGGCACAATAGTACCCATCAAGGGCAAAGACAGGAAGCCGGGGG
  ACCTGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172631
• Insert Size: 921 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172631.3, NP_766219.2
• RefSeq Size: 2493 bp
• RefSeq ORF: 921 bp
• Locus ID: 52662
• UniProt ID: Q8BWJ4
• Cytogenetics: 18 40.14 cM
• Gene Summary: Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal.[UniProtKB/Swiss-Prot Function]