Pura (NM_008989) Mouse Untagged Clone

CAT#: MC209266

Pura (untagged) - Mouse purine rich element binding protein A (Pura), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pura
• Synonyms: CAGER-1; Pur-alpha; ssCRE-BP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209266 representing NM_008989
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCGAGACAGCGGCAGCGAGCAGGGTGGTGCGGCGCTGGGCTCGGGCGGCTCCCTAGGGCACC
  CGGGCTCGGGCTCAGGCTCCGGCGGGGGCGGTGGTGGCGGCGGGGGCGGCGGCGGCAGTGGCGGCGGCGG
  CGGGGCCCCGGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGACATCCAGAACAAG
  CGTTTCTACCTGGACGTGAAGCAGAACGCTAAGGGCCGTTTCCTGAAGATCGCAGAGGTGGGCGCTGGCG
  GCAACAAGAGCCGCCTCACCCTCTCCATGTCTGTGGCCGTGGAGTTCCGCGACTACCTGGGCGACTTCAT
  CGAGCACTACGCGCAGCTGGGCCCCAGCCAGCCACCCGACCTGGCCCAGGCACAGGACGAGCCACGCCGG
  GCGCTCAAGAGCGAGTTCCTGGTGCGCGAAAACCGCAAGTACTACATGGATCTCAAGGAGAACCAGCGCG
  GCCGCTTCCTGCGCATCCGCCAGACAGTCAACCGGGGGCCCGGCCTGGGCTCCACGCAGGGCCAGACCAT
  TGCGCTGCCCGCACAGGGTCTCATCGAGTTCCGTGACGCTCTGGCCAAGCTCATCGACGACTATGGAGTG
  GAGGAGGAGCCGGCCGAGCTGCCCGAGGGCACCTCCTTGACTGTGGACAACAAGCGCTTCTTCTTCGATG
  TGGGTTCCAACAAGTACGGCGTGTTTATGCGAGTGAGTGAGGTGAAGCCCACCTACCGCAACTCCATCAC
  CGTGCCCTACAAGGTGTGGGCCAAGTTCGGACACACCTTCTGCAAGTACTCCGAGGAGATGAAGAAGATT
  CAAGAGAAACAGAGGGAGAAGCGGGCCGCTTGTGAGCAGCTCCACCAGCAGCAACAGCAGCAGCAAGAGG
  AGACCACCGCTGCCACCCTGCTACTGCAGGGCGAGGAAGAAGGGGAAGAAGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008989
• Insert Size: 966 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008989.3, NP_033015.1
• RefSeq Size: 1602 bp
• RefSeq ORF: 966 bp
• Locus ID: 19290
• UniProt ID: P42669
• Cytogenetics: 18 B2
• Gene Summary: This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.[UniProtKB/Swiss-Prot Function]