Cmklr1 (NM_008153) Mouse Untagged Clone

CAT#: MC208581

Cmklr1 (untagged) - Mouse chemokine-like receptor 1 (Cmklr1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cmklr1
• Synonyms: ChemR23; DEZ; Gpcr27; mcmklr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208581 representing NM_008153
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTACGACGCTTACAACGACTCCGGCATCTATGATGATGAGTACTCTGATGGCTTTGGCTACTTTG
  TGGACTTGGAGGAGGCGAGTCCGTGGGAGGCCAAGGTGGCCCCGGTCTTCCTGGTGGTGATCTACAGCTT
  GGTGTGCTTCCTCGGTCTCCTAGGCAACGGCCTGGTGATTGTCATCGCCACCTTCAAGATGAAGAAGACC
  GTGAACACTGTGTGGTTTGTCAACCTGGCTGTGGCCGACTTCCTGTTCAACATCTTTTTGCCGATGCACA
  TCACCTACGCGGCCATGGACTACCACTGGGTGTTCGGGAAGGCCATGTGCAAGATCAGCAACTTCTTGCT
  CAGCCACAACATGTACACCAGCGTCTTCCTGCTGACTGTCATCAGCTTTGACCGCTGCATCTCCGTGCTG
  CTCCCCGTCTGGTCCCAGAACCACCGCAGCATCCGCCTGGCCTACATGACCTGCTCGGCCGTCTGGGTCC
  TGGCTTTCTTCTTGAGCTCCCCGTCCCTTGTCTTCCGGGACACCGCCAACATTCATGGGAAGATAACCTG
  CTTCAACAACTTCAGCTTGGCCGCGCCTGAGTCCTCCCCACATCCCGCCCACTCGCAAGTAGTTTCCACA
  GGGTACAGCAGACACGTGGCGGTCACTGTCACCCGCTTCCTTTGCGGCTTCCTGATCCCCGTCTTCATCA
  TCACGGCCTGCTACCTTACCATCGTCTTCAAGCTGCAGCGCAACCGCCTGGCCAAGAACAAGAAGCCCTT
  CAAGATCATTATCACCATCATCATCACCTTCTTCCTCTGCTGGTGCCCCTACCACACCCTCTACCTGCTG
  GAGCTCCACCACACAGCTGTGCCAAGCTCTGTCTTCAGCCTGGGGCTACCCCTGGCCACGGCCGTCGCCA
  TCGCCAACAGCTGCATGAACCCCATTCTGTACGTCTTCATGGGCCACGACTTCAGAAAATTCAAGGTGGC
  CCTCTTCTCCCGCCTGGCCAACGCCCTGAGTGAGGACACAGGCCCCTCCTCCTACCCCAGTCACAGGAGC
  TTCACCAAGATGTCGTCTTTGAATGAGAAGGCTTCGGTGAATGAGAAGGAGACCAGTACCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008153
• Insert Size: 1116 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC100612, AAI00613
• RefSeq Size: 2858 bp
• RefSeq ORF: 1116 bp
• Locus ID: 14747
• UniProt ID: P97468
• Cytogenetics: 5 F
• Gene Summary: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B (By similarity). Positively regulates adipogenesis and adipocyte metabolism.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.