Pus1 (NM_001025562) Mouse Untagged Clone

CAT#: MC207521

Pus1 (untagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pus1
• Synonyms: A730013B20Rik; MPUS1; mPus1p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207521 representing NM_001025562
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAACAAGGTGCCACCAGCGCTGGCCTCACACCAACCGGACAGGAAGGGCCGAGGTGGCTGGG
  TCTGGGAGGAGACGGAGCATCCTGCAAAGAGGGTCAAGGGCGGCGAGGATGAAGAGCCCCCACGCAAGCT
  GCCAAAGCGAAAAATTGTGCTGCTCATGGCCTACTCGGGCAAGGGCTACCACGGCATGCAGAGGAATCTG
  GGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCGGCCTTAGTCCAGGCAGGTTGTATCCCTG
  AAAACCATGGCACAGATATGAGGAAGATGTCCTTCCAGCGGTGTGCTCGAACAGACAAGGGTGTGTCTGC
  AGCAGGGCAGGTGGTATCCCTAAAGGTGTGGCTGATTGATGACATTCTGGACAAGATCAACAGCCACCTT
  CCATCCCACATTCGGATTCTGGGATTGAAGAGGGTCACAGGCGGGTTCAACTCCAAGAACAAGTGTGACG
  CCAGGACCTACTGCTACATGCTGCCCACCTTTGCCTTTGCTCACAAGGACCGGGATGTACAGGACGAGAG
  CTATCGCCTGAGTGCAGAGACCCTGCAGCAGGTCAACCGCCTTCTGGCCTGCTACAAAGGCACCCATAAC
  TTCCATAACTTCACCTCGCAGAAGGGCCCAAGAGAGCCCAGTGCGCGCCGCTACATTCTGGAGATGTACT
  GCGAAGAGCCCTTCGTACGTGAGGGCCTGGAGTTTGCTGTGATCAAGGTGAAGGGCCAGAGCTTCATGAT
  GCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTGAAGGGTTATGCCCCTGAGAGTGTCCTG
  GAGCGCAGCTGGGGAGAGGAGAAGGTGGATGTGCCCAAGGCACCCGGGCTTGGCTTAGTCCTGGAGAGGG
  TTCACTTTGAAAAGTACAATCAACGCTTTGGCGGTGACGGGCTGCATGAGCCCCTGGACTGGACGCAGGA
  GGAAGGGAAGGTGACTGCTTTCAAGGAGCAGTACATTTACCCTACCATTGTCAGCACTGAGCGGGATGAG
  CGGTCCATGGCCCAGTGGCTCAATACTTTGCCGATCCACAACTTCAGTGGCACTGCACTTGGAGCGGCTG
  ACACAGGTGCCAAGGTCCCCAGTTCCCTGGAGGGCAGTGAAGGGGATGGAGACACTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025562
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025562.2, NP_001020733.1
• RefSeq Size: 1535 bp
• RefSeq ORF: 1182 bp
• Locus ID: 56361
• UniProt ID: Q9WU56
• Cytogenetics: 5 F
• Gene Summary: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:10094309). Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (PubMed:15327771). Isoforms 3 and 4 do not form pseudouridine when expressed in vitro (PubMed:11085940).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and 5' coding region and lacks an in-frame segment in the coding region, as compared to variant 1. These differences cause translation initiation at a downstream AUG and an encoded protein (isoform 3) that is shorter than isoform 1. Variants 3 and 6 both encode the same isoform (3).