Cyp2e1 (BC013451) Mouse Untagged Clone

SKU MC206516

Cyp2e1 (untagged) - Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (cDNA clone MGC:18528 IMAGE:4223797), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cyp2e1
• Synonyms: Cyp2e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013451; GTGGAGCCCCATCGGCACCATGGCGGTTCTTGGCATCACCGTTGCCTTGCTTGTCTGGATCGCCACCCTC CTCCTCGTATCCATCTGGAAACAGATCTATAGAAGTTGGAACCTGCCCCCAGGACCTTTCCCAATTCCTT TCTTTGGAAACATTTTTCAGCTGGATTTGAAGGATATTCCCAAGTCTTTAACCAAGTTGGCAAAGCGCTT CGGGCCAGTGTTCACACTGCACCTGGGTCAGAGGCGCATCGTGGTCCTGCATGGCTACAAGGCTGTCAAG GAGGTGCTACTGAACCACAAGAATGAGTTCTCTGGCCGAGGGGACATTCCTGTGTTCCAGGAGTACAAGA ACAAGGGGATTATTTTCAATAATGGACCCACATGGAAGGACGTGCGGAGGTTTTCCCTAAGTATCCTCCG TGACTGGGGAATGGGGAAACAGGGTAATGAGGCCCGCATCCAAAGAGAGGCACACTTCCTGGTGGAGGAG CTCAAAAAGACCAAAGGCCAGCCTTTTGACCCTACCTTTCTGATTGGCTGTGCACCCTGCAATGTCATTG CGGATATTCTCTTCAACAAACGCTTCGATTACGATGACAAGAAGTGTCTGGAGCTCATGAGTTTGTTCAA TGAAAACTTCTACCTGCTGAGTACTCCCTGGATCCAGGCTTACAATTACTTTTCGGATTATCTACAATAT CTACCTGGAAGCCACAGAAAAGTCATGAAAAATGTGTCTGAAATAAGACAGTACACACTTGGAAAAGCCA AGGAACACCTTAAGTCACTGGACATCAACTGCCCCCGGGATGTGACTGACTGTCTCCTCATAGAGATGGA GAAGGAAAAACACAGCCAAGAACCCATGTACACAATGGAAAATATTTCTGTGACTTTGGCCGACCTGTTC TTTGCAGGAACAGAGACCACCAGCACAACTCTGAGATATGGGCTCCTGATTCTCATGAAATACCCAGAAA TTGAAGAGAAACTTCATGAAGAAATTGACAGGGTTATTGGGCCAAGCCGTGCCCCTGCAGTCCGAGACAG GATGAATATGCCCTACATGGACGCTGTAGTGCATGAGATTCAGAGATTCATCAACCTCGTCCCTTCCAAC CTGCCCCACGAAGCAACCCGAGACACCGTGTTCCGAGGATATGTCATCCCCAAGGGTACAGTTGTAATTC CAACTCTGGACTCCCTTTTATTTGACAACTATGAGTTTCCAGATCCAGAGACATTTAAACCTGAGCATTT TCTGAATGAAAATGGGAAGTTCAAGTACAGTGACTATTTCAAGGCGTTTTCTGCAGGAAAGCGCGTGTGT GTTGGAGAAGGCCTGGCCCGCATGGAACTGTTTCTGCTTTTGTCTGCTATTCTGCAGCATTTTAATCTGA AGTCTCTGGTTGACCCTAAGGATATCGACCTCAGCCCTGTTACAATTGGCTTTGGCAGTATCCCACGCGA ATTTAAACTCTGTGTCATTCCTCGTTCATGAGACCTGCTGCCCATCATTATCCCTTACAAAATGACTGTT TAAAAAAATGCCAAGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC CGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTATCGAGATCTGACTCTGTCTTCTG GAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAGTCTTTAAAAAAAAAAAAAAAA AAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC013451
• Insert Size: 1482 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC013451, AAH13451
• RefSeq Size: 1759 bp
• RefSeq ORF: 1482 bp
• Locus ID: 13106
• Cytogenetics: 7 85.94 cM
• Summary: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.[UniProtKB/Swiss-Prot Function]