Samhd1 (BC012721) Mouse Untagged Clone

CAT#: MC206493

Samhd1 (untagged) - Mouse SAM domain and HD domain, 1 (cDNA clone MGC:14068 IMAGE:4037046), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Samhd1
• Synonyms: E330031J07Rik; Mg11
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012721
  CCACGCGTCCGCTGCGCGCGAGCCGGTGCCGCGGTACTTAACTTCGCAGCCCCGGGTGTCGGAGGTAGCC ATGCAGAGCGCACCCTTGGAGCAGCCAGCTAAGCGACCCCGCTGCGATGGCAGCCCAAGGACGCCACCGA GCACTCCTCCTGCAACAGCTAATCTGTCTGCAGACGACGACTTCCAAAACACCGACCTCCGAACCTGGGA ACCGGAGGACGTGTGCTCCTTCTTAGAGAATCGTGGTTTCCGAGAGAAGAAAGTGCTGGACATCTTCAGA GACAATAAAATCGCCGGCTCGTTTCTGCCCTTTTTGGATGAGGATCGTCTGGAAGATCTGGGAGTAAGTT CCTTGGAGGAGAGGAAGAAGATGATAGAATGTATCCAGCAGCTGAGTCAGTCTCGGATTGATCTAATGAA GGTATTTAATGATCCCATTCATGGCCACATTGAGTTCCACCCTCTCCTTATCAGAATCATCGACACACCT CAGTTCCAGCGACTTCGCTATATCAAGCAGCTGGGGGGCGGCTACTATGTTTTCCCTGGAGCGTCCCACA ATCGCTTCGAACACAGTCTCGGAGTGGGGTACCTAGCAGGCTGCCTGGTGCGAGCACTTGCCGAAAAACA GCCAGAGCTACAGATCAGTGAGCGAGATATACTCTGTGTTCAGATTGCGGGGCTCTGCCACGACCTAGGT CATGGGCCATTTTCTCATATGTTTGATGGAAGGTTTATCCCACGGGCTCGCCCAGAGAAAAAGTGGAAGC ACGAACAGGGCTCCATTGAGATGTTTGAGCATCTGGTCAATTCTAATGAACTCAAACTTGTCATGAAGAA CTATGGTCTCGTCCCTGAAGAAGACATTACCTTTATCAAGGAACAAATTATGGGACCACCTATAACACCA GTCAAAGATTCCTTGTGGCCGTATAAAGGCCGCCCTGCCACGAAGAGCTTCCTTTACGAGATAGTGTCTA ACAAGAGGAATGGCATCGACGTAGACAAATGGGATTATTTTGCCAGAGACTGTCACCATCTTGGAATCCA AAATAATTTTGATTACAAGCGCTTCATTAAGTTTGCCCGTATCTGTGAAGTGGAGTACAAGGTCAAGGAG GACAAGACCTACATCCGTAAGGTGAAGCACATTTGTTCGAGAGAAAAGGAGGTTGGAAATCTGTATGACA TGTTCCACACTCGCAACTGCTTACACCGAAGAGCTTACCAACACAAGATCAGCAACCTCATCGACATAAT GATTACAGATGCCTTCCTCAAAGCAGACCCCTACGTGGAGATTACAGGGACTGCCGGGAAGAAGTTCCGC ATTTCCACAGCCATTGATGACATGGAAGCCTTCACTAAGTTGACGGATAACATTTTTCTGGAGGTTTTAC ACTCTACTGATCCACAGTTGTCTGAGGCCCAAAGTATTTTAAGGAACATTGAATGCCGCAATCTATACAA GTATTTGGGTGAGACCCAGCCAAAGCGTGAGAAGATTAGGAAGGAAGAATATGAACGGCTTCCCCAAGAA GTTGCTAAAGCCAAACCTGAAAAAGCCCCGGATGTTGAACTGAAGGCCGAAGATTTCATAGTTGATGTTA TCAATGTGGATTATGGGATGGAAGACAAGAATCCAATCGATCGTGTTCACTTCTATTGTAAGAGCAACAG CAAGCAAGCGGTCAGGATCAATAAAGAGCAGGTGTCACAACTGCTGCCAGAGAAATTTGCAGAGCAGCTC ATTCGGGTGTACTGTAAGAAGAAAGACGGGAAGAGCCTGGACGCCGCCGGGAAGCACTTTGTTCAGTGGT GTGCGCTCAGGGACTTCACCAAGCCACAGGATGGTGACATTATAGCTCCACTCATAACACCTCTGAAATG GAATAATAAGACTTCATCTTGCCTCCAAGAAGTATCCAAAGTAAAAACATGTCTAAAATTTTAAAAAAAA AAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC012721
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012721, AAH12721
• RefSeq Size: 1967 bp
• RefSeq ORF: 1884 bp
• Locus ID: 56045
• Cytogenetics: 2 H1
• Gene Summary: Isoform 1: Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (By similarity). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed:23972988, PubMed:23872947, PubMed:26667483, PubMed:29379009). Likewise, suppresses LINE-1 retrotransposon activity (PubMed:26667483). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (By similarity). Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (By similarity). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (By similarity). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (By similarity). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (By similarity). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (PubMed:29669924).[UniProtKB/Swiss-Prot Function]