Tspan12 (BC068240) Mouse Untagged Clone

CAT#: MC206421

Tspan12 (untagged) - Mouse tetraspanin 12 (cDNA clone MGC:25702 IMAGE:3674585), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tspan12
• Synonyms: 9030619E17; AI426782; AI663988; AW111457; Tm4sf12
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068240
  GGAAGACAAGGAAGCGTCTGCTGCTGGCGCCGATGCATCCCTTTGGCTCTCCGGGAGGAGCGTCCCTGCC CGGATGAGTGTCTGCTGTGTGTCCCCGACTGTTCCCCGGCAGGAGCGCAGCTCGAACGATCGCCGGGGCT CTGCCTGGACCAGTAGAATGTGGTGAGAGGATGGGGTTTCTCCCTCCCAAGACTCACAATGGCCAGAGAA GATTCCGTGAAGTGTTTGCGCTGCTTGCTCTACGCCCTCAACCTGCTCTTTTGGTTAATGTCCATCAGTG TCTTGGCAGTTTCTGCTTGGATGAGGGACTACCTGAATAATGTTTTGACTTTAACTGCAGAAACAAGGGT AGAAGAAGCGGTCATCTTAACTTACTTCCCCGTGGTTCACCCCGTCATGATTGCTGTCTGCTGCTTCCTT ATCATCGTGGGAATGCTGGGATACTGTGGAACGGTGAAAAGAAATCTGTTGCTTCTTGCATGGTACTTCG GAACGTTACTCGTCATCTTCTGTGTAGAACTGGCTTGCGGTGTGTGGACATACGAGCAGGAGGTTATGGT GCCAGTACAGTGGTCAGATATGGTTACTTTGAAAGCCAGAATGACAAATTACGGCTTACCGAGGTATAGA TGGCTTACACACGCTTGGAATTATTTTCAGAGAGAGTTTAAGTGCTGTGGAGTGGTGTACTTCACTGACT GGTTGGAAATGACAGAAATGGACTGGCCTCCCGACTCCTGCTGTGTCAGGGAGTTCCCAGGGTGCTCCAA GCAGGCTCACCAGGAAGATCTCAGTGACCTTTACCAAGAGGGTTGTGGGAAGAAGATGTATTCCTTTTTG AGAGGAACCAAACAATTGCAAGTTCTAAGGTTTCTGGGAATCTCCATTGGTGTGACACAAATCCTGGCCA TGATTCTCACCATTACTCTGCTCTGGGCTCTGTATTATGATAGAAGGGAGCCTGGAACAGACCAAATGCT ATCTCTGAAAAATGATACGTCTCAGCACTTGTCATGTCATTCTGTGGAACTGTTAAAACCAAGTCTTTCA AGGATCTTTGAGCATACATCAATGGCAAACAGCTTCAATACACATTTTGAGATGGAAGAATTATGAAGAA TGTCAAAGAAGGAAATCACAAACTTGTGGGACTAGTGGGTTTTGGGGTACACAGTTATCTATTTCAGAAA TTTTTAGACATATTAATATTCTGAAAAGGAATGTCTAAGAATTTCTCTACTCTTTAAAACAAAGGTTCCA CATGGTAATACACAGACAATAATTGGTGCCCTTCAAAATGCCGAACCCAGACATTATAACAACTGTGTGT GCTTTCCTTTCTTTTCTTTTTTTCTGAGCACAATTATGTTTTGCAGTGATACAGTTTGACCAGCGTTTCT GCATCCATTCACAGAAATGAACCGTCATGGTGGCACTGGAGCTGATATGAAGGTTAACTGACTCCTCTAA CTAACCTATAAACTATCCACTGACTGCTAACATAGGGATTTAGTGCTAAAGACCTTTATTAGCTGGTGAT GTATTGTTCTGATGAGGTATTTGATTATATGCAAAAATCTTTCAAGATTGGTGACTACCTAAATATGATT TTTGTTGGTTACTAAAATAATCTTATTAATAATACTTGTAAGAGCTAACATGTTGTCTTAAACTTATCAG GGATATATATGTATATAAGTCTGTGCCGTGTCTGTATAATTCAGTAGATTTCAGTTCCTATAATATTATG AAAAACAATATTTGAAAATGGCGAAAATTGTCTTACACAGCACCATTACTTTTGTCTCTTTCAGCAACAG AACTTTTAAATTCCGTCTTGGTTCTGTACTATATCTATAACTGTTAATTTAAGTTGTTAACCACTAATTT TGGAAATTACCAGTTTGACACATAGGAATCTCTGTAAGTCTGCCTGCAGGAAGTATTAACTTCAGAATTT GCACATAGTTGATTCATCAAAAGTTCAGATTTTTCCCCCTCCCAATAAACCCTTTGTCAAAGAGTGTTTT TCATTTTTATTTTTAATGTTTTCATGTATTTTTTTGCATTCAAGAATCAACAACTTGAAGTTGATGTAAT TTTCCAGAGAAACAACATTCTCTGAAGAGATGCTCATAGGAATCTCTAAAATAAGTGGTAGCCTAACCAG AGAGAATTCTTGATTTCTTTCATCAATTAATACACTGTAGCAAATGATACAGAATATTAATTTTTTACAA GAGTAGAAGATATTTATTTGAAACGGGAAAAGTGCATTTTACTGTATCTTGTGCATTCTGTTCATTTCTT GGATTACAGAAAGAAAATTAATCAATAAATATTTTCTAGATCGTGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC068240
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068240, AAH68240
• RefSeq Size: 2388 bp
• RefSeq ORF: 918 bp
• Locus ID: 269831
• Cytogenetics: 6 A3.1
• Gene Summary: Regulator of cell surface receptor signal transduction. Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity (By similarity). Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1).[UniProtKB/Swiss-Prot Function]