Arhgef18 (BC060639) Mouse Untagged Clone

CAT#: MC206181

Arhgef18 (untagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (cDNA clone MGC:79199 IMAGE:6849875), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef18
• Synonyms: AI467246; D030053O22Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060639
  GCGGAGACTGCGAGAGCGACCCGGGAGCCAGCAGGGCGGAGAGCGGCCTGTGAGGGACCCCGCGGAGCCT CCCTGAAGGAGCACCCTCGGACCACACTTCTGTCTGATGGCAGCAGCCCAGCTCCTTCCAGGAATGTCGG CATGACCATCTCTCAGAAAGGGGGTCTCCAGCCAACACCGAGCCCAGCTGGATCTGGGGTGCGACTTGGA CCAATTGCTGGAGACATGGATGAAGCTGACTCCGTGTTTTTAAAGTTGAAGCAGACGGCAGACGACTCTC TGTCACTCACATCCTCGAATGCTGAGTCTGTTTTCATAGAAGACCCTTACATTGCTTCACTGAGGTGTGA GATCGAATCAGATGCACATGAGTTTGAGGCCGAGTCCTGGAGCCTCTCTGTGGATTTAGCATATGCCAAG AAACAGAAGAAGGAGGTGGTAAAGAGACAGGACGTCCTATATGAGCTGATGCAGACAGAGGCTCATCACG TGCGCACACTAAAGATCATGCTGAAAGTATACTCCAGAGCCCTGCAGGAAGAGCTGCAATTCAGTGGCCA GGCAGTCAGCCGTCTGTTTCCGTGCGCTGATGACTTACTGGACATGCACAGCCATTTCCTTGCCCGCCTT AAGGAGCGCCGGCAGGAGTTCCTAGAGGAGGGCAGTGATCGGAATTACGTCATCCAAAAGATCGGAGACG TCCTGGTTCAGCAGTTCTCAGGAGAAACCGGGGAGAGAATGAAGGAGAAATACGCTGTGTTCTGTAGTGG CCACAATGATGCAGTCGGTCATTACAAGCTATTGCTGCAGCAAAGCAAGAAGTTTCAAAACTTAATCAAG AAAATTGGCAACTTTTCCATTGTGCGAAGGCTGGGAGTGCAGGAGTGCATCCTTCTGGTCACACAGCGCA TAACCAAGTACCCGGTGCTTGTGGAACGCATCATCCAGAACACAGAAGCTGGCACTGAGGACTACAAGGA CCTGAGCCAAGCCCTGAGCCTCATCAAAGACATCATCTCACAAGTGGACGCCAAAGTCAGTGAGTATGAG AAGGACCAGCGCCTCAAGGAGATTGCAGCCAAGACGGACCAGAAGTCGTCTGGCAAACTCAAAAACGGGC TAACCTTCCGAAAGGAGGACATGCTGCAGCAGCGCCAGCTCCACCTGGAAGGCGCCCTCTGCTGGAAGTC CACCTCAGGGCGCCTGAAAGATGTCCTCGCTGTCCTGCTGACCGATGTGCTTTTGCTGCTGCAAGAAAAA GATCAGAAATATGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCTCTGCAAAAGCTCATCGTGA GAGAGGTAGCCAACGAGGAGAAGGCCATGTTCCTGATCAGCGCCTCCATGCAGGGGCCAGAGATGTATGA GATGTACACCAGCTCCAAAGAGGACAGGAACATCTGGATGGCCCACATCCGCCGGGCCGTGGAGAGCTGT CCTGACGAGGAGGAGGACGTTTTCAGTGAGGCTGAGGAGAAAAAGATAGCTGAAGCCCGCACCATGAAAC TCCAGGAATTTCAAGAGCGACTGAGCCTGAAAGACCAGCTGATTGCCCAGAGCCTTCTGGAGAAACAGCA GATCTACCTGGAGATGGCTCAGCTGAGTGGGCTGGAAGAGTCAGCCCAGAACCGAGGCCTCTTCCGAGGA GGAGGAGACCCCTCTGAGACCCTGCGGGGGGAGCAGATTCTCAGATCGGCCATGAGTGAGATTGAGGGCA TCCAGAGCCTGATCTGCCAGCGGCACCTGGGCAGCACCAGTAGCCAAGTGGAGGAGGGAAGTGTCTCTGC AGGCCTGCCTCGGCGGGCTGAGACCTTCGGGGGCTATGACAGTGTGGGAAGCCCTAGCAAGGGTGGCAGC TTTAAAAGGAAGGTATCCAACAGTGACCTCAGGCCCCAAGACTGGCAGGGACCTGCTAGCAGCCCAGACT CCAGGCCCTGTGACAACAGCGCCCCCAGTGGCTGCTGTGAGGAATCGCCACAGGCTGTGGAGATGCCTAG CACAGAGAGCCTGCCCACTGTCCTGGAATTAGAGCTGGTCCACCGGGTCCAGACACTGTCGCAGTTGCTT CTTAGTCTCCAGGCGGTCATCGCTCAACAGGATAGCTATGTGGAGATGCAAAGAACGGCCATCCAGGAGC GCGAGAAGCAGTTCCGGCTGCAGTCAACACGTGGGAACCTGCTGCTGGAGCAAGAGCGGCAACGCAACTT TGAGAAGCAGCGTGAGGAGCGCGCCGGTGTGGAGAAGCTGCAAAGCCAACTTCGCCAGGAGCAGCAGCGA TGGGAGCGGGAGCGGGCACGCCAGCAGCAGGAACTGGAGCTGGCCGGTGCGCGACTGCAGGAGCGAGAGG GCGAGGCACGCCAGATGCGCCAGCGGCTGGACCAGGAGCGCACCGAGCTAGAGCGACAGCGCCAGGCCTA CCAGCATGACCTGGAGCGTCTGCGTGAGGCCCAGCGAGCAGTGGACCGTGAACGTGAGCGCCTGGAGCTG CTGCGCAGGTTCAAGAAACAGAACACTGTACCAGGGGCACTGCCCCCGGAGGTGCTGGCAGAGGCCCAAC CTGCAAGCCACCCTCCCAGCTTCAATGGCGATGGGCTGGAGGGGCACTCGGCCCCAGCCAAAGCACCAGG AACCCAAGGGAGCGCGATGCTACATGGCACAGGACCTGACAACGTAGAGCGACCCGAGGTGGCCCGCTGG GACAGTGCCCCCCCTGAGAGCCGGCCAGCCAAGAGCGATGTGCCCATCCAGCTACTCAGTGCCACCAATC AGATTCAGAGGCAGACAGCCGTGCAACAGCAGATCCCCACCAAGCTGGCCGCCTCCACCAAGGGTGGCAA GGAGAAGGGCAGCAAGAGCAGGGGTTCCCAGCGTTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAGCAG CTGCTCCTCAGCAAGTTCATAGGCAAGGATGAGAGTGCATCACGGAACCGCCGTTCACTGAGTCCCGTCC TGCCTGCCGCCCATGGGTCTGCACCTGCCTCAGACCCCTGCTTCCCAGCCCCAAGCCCGGCCCCAGCTGC CACACCCCCCGAGGCCTTCAAGTTCGGAGGCACATCACTACCACCTGTCTCCCCAGCTTCCTCGCTGCCA ACCACACCACTCGCCACCACAGATGAAGTTAGTAAGGAAGATGTCATCTTCTTCTGAGGGGCCATGGCCC CAAGGGCAGACCCCTTTCTGTCCTGATACCCTTTATGTCTCTGGGGATCCTATGGGTGACTGTACTGTCT GGCCGTCCCCTTCCCCTGCACAAGCACAGCCAGGCACTTGTTCCCTTGAGATTGCCCAAATGGTATGCCT TGAGTCTCACAGCTCGCTATAGATTTAGTGGCAAAACATGGGTAGCCTTGAGTCTCACAGCTCGCTATAG ATTTAGTGGCAAAACATGGGTAGCCCCCTTGAACACACATACATACATGCATATATATGTATATGTGTGT GTGTATATATATATATATTATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC060639
• Insert Size: 3066 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060639, AAH60639
• RefSeq Size: 3568 bp
• RefSeq ORF: 3066 bp
• Locus ID: 102098
• Cytogenetics: 8 A1.1
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.[UniProtKB/Swiss-Prot Function]