Acp2 (NM_007387) Mouse Untagged Clone

CAT#: MC204487

Acp2 (untagged) - Mouse acid phosphatase 2, lysosomal (Acp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acp2
• Synonyms: Acp; Acp-2; LAP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023343
  CAGTGATGGCCGGCAGACAGACTGGTTGGAGCCAGGCGGCTCTTCTCCAGTTCCTTCTTGGCATGTGCCT AACGGTGATGCCACCCATACAGGCCCGGAGTCTACGCTTTGTTACCTTGCTGTATCGACATGGAGATCGG TCACCAGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAA CCAAGGAAGGGATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAA CACCTCTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAG GCCAACCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTA TCCCTGTTCACACTGTGCCCATCACTGAAGACAGGTTGCTGAAGTTTCCTTTGGGTCCATGTCCCCGTTA TGAGCAGCTGCAGAATGAGACTCGGCAGACACCAGAGTATCAGAACAGAAGTATTCAGAATGCACAATTT CTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACA CACTCTTTTGTGAGCAAACCCATGGGCTGCTTCTGCCGCCCTGGGCCTCACCCCAAACCGTGCAGCGTCT GAGCCAGCTAAAGGACTTCAGCTTCCTCTTCCTCTTCGGGATCCACGAGCAAGTACAGAAGGCCCGGCTT CAGGGGGGAGTTCTGCTGGCTCAGATATTGAAGAACCTGACCCTAATGGCAACTACCTCTCAATTCCCTA AGCTTCTGGTTTATTCCGCGCATGACACTACCCTGGTTGCTCTGCAAATGGCATTGAATGTCTACAATGG GAAGCAAGCTCCCTATGCTTCCTGCCACATATTTGAACTGTACCAGGAAGATAATGGGAATTTCTCAGTC GAGATGTACTTTCGGAATGACAGTAAGAAGGCACCCTGGCCTCTGATCCTGCCTGGCTGTCCTCACCGTT GCCCACTGCAGGATTTCCTTCGCCTCACAGAGCCTGTCATACCCAAGGACTGGCAGAAGGAGTGCCAGCT AGCAAATGATACTGCAGACACAGAGGTAATTGTGGCATTGGCTGTCTGTGGCTCCATCCTCTTCCTTCTC ATAGTGTTGCTCCTCACCATCCTCTTCCGGATGCAGGCCCAGCCTCCTGGCTACCACCATGTTGCAGACA GGGAAGACCATGCTTGACAACCACTCAGTCCCCTTCCCTCTGCCTCCTAGGGGAGGTGGGCTGAGTCCTC GCTCCTGACCATTGCTGATCCCAGCCCAGGGACAGGGTTAGCCTTCCTGATTACCTACGCCCAAATGAAT GAGTGAGGCTCGGCTGGGTGTGCGGCACCTGGGGCTCACCATCTTCATGCCTGATGTTTACCGTGTACTG TGTTGGACACTGGCTTTCTCTAAATGGGATTTGCCTCTTCTATATTCCCTAAGGACTTGAGATGCAGACA AGCATTCAGATTTCACTCAAGACCTGGGACTAAAAAACAAAACAACAAAACCCCCATGAAAACCTGAAGA AGCTGGTACTTGATCTGCCTTGCTCTTCCACCGAGCCTTGCTCTTTATCTTCTAGCCCATATCTTTGGCA AATGGCTTGGAGGACATTGTCCCTTGGTACTTTTTTAGAGGCAAAAACTGCAAATCACTAGGAGTATAAA ATATGGCTATCAAGAACTCGGGTCATCCAACAGCAAAGTCATCCTTTGGCTGAGGCTACCGTCAGTGTCA ACCACAGGCAGGTCTGAAGGATACTTGGGCTGTACCTGGATGACAGTGGGAGGACACAGGTAGGAGTGAA ACACATGGAATCATGAGGTGACTCTCCTCAGCTGGGCTGGACTCTTCAGGGACCGTACACCTTGACCGTG GCCCCAAATAGGGCTGAAGTAGCTCCGAGGAAATGAAGGTTTTGACTGTGAGTGCTGGTTCTGTGCCTTT TTTCCCTTCCCCATTTCTGACATCCAAGAAGCACTCTTACCAGGTCAGTACTAGATCTACTCAGAAACCC TGTTGGGCTTTTGGGTTTTTAGTTAGATACAAAGGATATGCTTTTATTGGCCATGCTAAAATTGAACATA AAAGTTTTAAAATTCTCTGGTTATTTTGCTCCTATACATCAGAAAAAAGAAAAGTACCTTGGCTCTAGCA TAAACTTGGCCTCCAGGCTAGATATAGCTGGGACCCTGAGCGTAGCTGGTTTACTCTTTAATTTTCTGGA GCAAAATCTAGAGCAGTAACATGTCAGCATCCTAGTCCCACAGTATCCCTTGTCACAAGTGTCCCTGCTC CAACCCTAAGAATTGGGTACTTTTGGCCTATTAACAAACAGCTGCCACTCCTCACAGAATGTGTTCCCAT CTGTGGGGTCCAGTATTGCTGTCCCACACCATCTTCATAGTCTTTCATGATCTTTCTGTGACCCATCTTC CTCTTGGCTCCAAATGAGAATATGATAACCTAGAAGAAACAAGAAGTCTGAATGACACCTTCTGTTGCTG GTCTGTCTCAGAGGTTGGGGCTGTCTTGGGGAACTAACAGACTGACATTGCCATGACTAAAATAAAACCA GATGGGTGACCTAGGCCAGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007387
• Insert Size: 1272 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023343, AAH23343
• RefSeq Size: 2695 bp
• RefSeq ORF: 1272 bp
• Locus ID: 11432
• UniProt ID: P24638
• Cytogenetics: 2 50.54 cM
• Gene Summary: The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]
  Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1).