Fyn (NM_008054) Mouse Untagged Clone

CAT#: MC204024

Fyn (untagged) - Mouse Fyn proto-oncogene (Fyn), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fyn
• Synonyms: AI448320; AW552119
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC032149
  CCACGCGTCCGAAACAAAGCAAAGGAACAGGTGGTGGGGAGTTTGCTGCATGGATGGGTGCCCCAATCGA TCGATCGAGTCAGTCCGACCGCTGGTTTCGAAGACATGTGGTGTATATAAAGTTTGTGATAGTTGGTGGA AATTTGGGAGCTTGGATAATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAGCGAAACTGACAGAGGAGA GGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAG CTTCGGCGTGACCTCCATCCCGAACTACAACAACTTCCACGCAGCTGGGGGCCAGGGACTCACCGTCTTT GGGGGTGTGAACTCCTCCTCTCACACTGGGACCCTACGCACGAGAGGAGGGACAGGAGTGACACTGTTTG TGGCGCTTTATGACTATGAAGCACGGACGGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAAT ATTGAACAGCTCGGAAGGAGACTGGTGGGAAGCCCGCTCCTTGACAACCGGGGAAACTGGTTACATTCCC AGCAATTACGTGGCTCCAGTTGACTCCATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGCAAAG ATGCTGAGAGACAGCTCCTGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGCGAAACCAC CAAAGGTGCCTACTCACTTTCCATCCGTGATTGGGATGATATGAAAGGGGACCACGTCAAACATTATAAA ATCCGCAAGCTTGACAATGGTGGATACTATATCACAACGCGGGCCCAGTTTGAAACACTTCAGCAACTGG TACAGCATTACTCAGAGAAAGCTGATGGTTTGTGTTTTAACTTAACTGTGGTTTCATCAAGTTGTACCCC ACAAACTTCTGGATTGGCTAAAGATGCTTGGGAAGTTGCACGTGACTCGTTGTTTCTGGAGAAGAAGCTG GGGCAGGGGTGTTTCGCTGAAGTGTGGCTTGGTACCTGGAATGGAAATACAAAAGTAGCCATAAAGACCC TTAAGCCAGGCACCATGTCTCCGGAGTCCTTCCTGGAGGAGGCGCAGATCATGAAGAAGCTGAAGCATGA CAAGCTGGTGCAGCTCTACGCGGTCGTGTCTGAGGAGCCCATTTACATCGTCACGGAGTACATGAGCAAA GGAAGTTTGCTTGACTTCTTAAAAGATGGTGAAGGAAGAGCTCTGAAGTTGCCAAACCTTGTGGACATGG CGGCACAGGTTGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCACAGAGATCTGCGATCAGC AAACATTCTAGTGGGGAATGGACTAATTTGCAAGATTGCTGACTTTGGATTGGCTCGGTTGATTGAAGAC AATGAATACACAGCAAGACAAGGTGCGAAGTTTCCCATTAAGTGGACAGCCCCCGAAGCGGCCCTGTATG GAAGGTTCACAATCAAGTCTGACGTATGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAG AGTGCCATACCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGAGAGGCTATAGGATGCCCTGC CCACAGGACTGCCCGATCTCCCTGCACGAGCTCATGATCCACTGCTGGAAAAAGGATCCGGAAGAGCGCC CGACCTTCGAGTACTTGCAGGGCTTCCTGGAGGACTACTTTACGGCCACAGAGCCCCAGTATCAGCCCGG TGAAAACCTGTGAGAGCCTGCGCTTCAGACGCCTCTTCCCGAGGCCTCCCTACCCCTCCCCATTAGCTTC CAATTCTGTAGCCAGCTGCCCCAGAGCAGCGAGAACCGTCCAGGATCAGATTGCATGTGACTCTGAAGCT GAACTTCCACGGCCCTCATTAATGACACTTGTCCCCCAGTCCGAACCTCCTCTGTGAACCATCTGAGACA GAAGCGTGTTATTTCTCAGACTTGGAAATGCATTGTATCGATGTTATGTCAAAGGCCAAACCTCTGTTCA GTGTAAATAGCTGCTCCTGTGCCAACAATCCCAGTGCTTTCCTTTTTTAAAAAAGAAAAAGCAAATCCTA TGTGATTTTAACTCTGTCTTCACCTGATTCAACTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008054
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032149, AAH32149
• RefSeq Size: 2149 bp
• RefSeq ORF: 1605 bp
• Locus ID: 14360
• UniProt ID: P39688
• Cytogenetics: 10 20.51 cM
• Gene Summary: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame exon in the coding region compared to variant 1. It encodes an isoform (b) with a different and shorter internal segment compared to isoform a. Variants 2 and 3 encode the same isoform (b).