Acaa2 (NM_177470) Mouse Untagged Clone

CAT#: MC203155

Acaa2 (untagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acaa2
• Synonyms: 0610011L04Rik; AI255831; AI265397; D18Ertd240e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028901
  GACGCGTGGGCCGCGGAATAGCTGAGCTTCGCCATGGCCCTGCTACGAGGTGTGTTCATCGTCGCTGCGA AGCGAACACCCTTTGGAGCTTACGGGGGCCTTCTCAAGGACTTCTCTGCCACCGATTTAACTGAATTTGC TGCCAGGGCTGCTCTGTCTGCTGGCAAAGTTCCACCTGAAACCATCGATAGTGTCATCGTGGGCAATGTC ATGCAGAGCTCTTCAGATGCGGCATACCTGGCGAGGCATGTGGGTTTGCGAGTGGGAGTCCCAACAGAGA CTGGGGCACTTACCCTCAACAGGCTCTGTGGCTCTGGTTTCCAGTCCATCGTGAGCGGATGTCAGGAAAT CTGTTCTAAAGATGCTGAGGTCGTCTTGTGTGGAGGAACAGAGAGCATGAGCCAGTCCCCCTACTGTGTC AGAAATGTGCGCTTCGGAACCAAATTTGGATTAGATCTCAAGCTGGAAGATACTTTGTGGGCAGGATTAA CGGATCAACATGTTAAGCTGCCCATGGGAATGACTGCAGAGAACCTTGCTGCAAAATACAACATAAGCAG AGAAGACTGTGACAGATACGCCTTGCAGTCTCAGCAGAGGTGGAAAGCTGCTAACGAGGCTGGCTACTTC AATGAGGAGATGGCACCCATTGAGGTGAAGACGAAGAAAGGCAAACAGACCATGCAAGTGGACGAGCACG CTCGACCCCAAACCACCCTGGAGCAACTGCAGAAGCTCCCGTCCGTGTTCAAGAAAGACGGGACAGTCAC AGCAGGGAACGCCTCGGGGGTGTCTGACGGTGCTGGGGCCGTCATCATAGCCAGCGAAGATGCTGTCAAA AAACATAACTTCACGCCCCTGGCCAGAGTCGTGGGCTACTTCGTGTCCGGATGCGATCCTACTATCATGG GTATTGGTCCAGTCCCTGCTATCAATGGAGCATTGAAGAAAGCTGGGCTGAGTCTTAAGGACATGGATTT GATAGACGTGAACGAAGCTTTTGCCCCTCAGTTCTTGTCTGTTCAGAAGGCCCTGGATCTTGACCCCAGC AAAACCAATGTGAGTGGAGGCGCCATTGCCCTGGGTCACCCGCTGGGAGGATCTGGCTCCAGAATCACCG CACACCTGGTTCATGAGTTAAGGCGTCGAGGTGGAAAGTACGCAGTGGGATCGGCTTGCATTGGAGGTGG CCAAGGCATCGCCCTGATCATCCAGAACACAGTCTGAAGGCATCACAAGCACACTGCCCACACTTACTGG GCCAGGCCACGGAACACAGGAGACCTTCAAGTCAGCCCTGCTGAGACAGTGATTATATGTGACCAAGCCT TGATGAGGCAAGATGCATTGGGTTCTGTCTACTTTATACCTGCCTGACGTGTTAGAATAAAAACACCAAC CATCGGAGGCCTTAAGAGAAATGGTATCTGTCAGTAGTCACCACTGTATGCCTTCCATGGAGTAATACAA ACTGAATAAATGTTGCCTTAACTCCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_177470
• Insert Size: 1194 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028901, AAH28901
• RefSeq Size: 1510 bp
• RefSeq ORF: 1194 bp
• Locus ID: 52538
• UniProt ID: Q8BWT1
• Cytogenetics: 18 50.76 cM
• Gene Summary: In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs (By similarity). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (By similarity). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity).[UniProtKB/Swiss-Prot Function]