Ido1 (NM_008324) Mouse Untagged Clone

SKU MC202318

Ido1 (untagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Ido1
• Synonyms: Ido; Indo
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049931 sequence for NM_008324; AGCAATGGCACTCAGTAAAATATCTCCTACAGAAGGTTCTAGAAGGATCCTTGAAGACCACCACATAGAT GAAGATGTGGGCTTTGCTCTACCACATCCACTGGTGGAGCTGCCCGACGCATACAGCCCCTGGGTCCTTG TGGCTAGAAATCTGCCTGTGCTGATTGAGAACGGGCAGCTTCGAGAAGAAGTTGAAAAGCTGCCCACACT GAGCACGGACGGACTGAGAGGACACAGGTTACAGCGCCTGGCACACCTGGCCCTGGGGTACATCACCATG GCGTATGTGTGGAACCGAGGGGATGACGATGTTCGAAAGGTGCTGCCCCGCAATATTGCTGTTCCCTACT GCGAGCTCTCAGAGAAGTTGGGCCTGCCTCCTATTCTGTCTTATGCAGACTGTGTCCTGGCAAACTGGAA GAAAAAGGACCCCAATGGGCCCATGACATACGAGAACATGGACATTCTGTTCTCATTTCCTGGTGGGGAC TGCGACAAGGGCTTCTTCCTCGTCTCTCTATTGGTGGAAATCGCAGCTTCTCCTGCAATCAAAGCAATCC CCACTGTATCCAGTGCAGTAGAGCGTCAAGACCTGAAAGCATTGGAAAAGGCACTGCACGACATAGCTAC CAGTCTGGAGAAAGCCAAGGAAATTTTTAAGAGGATGCGTGACTTTGTGGACCCAGACACGTTTTTCCAC GTTCTCCGCATATATCTGTCTGGCTGGAAATGCAGCTCCAAGCTGCCAGAAGGTCTGCTGTATGAGGGGG TCTGGGACACCCCAAAAATGTTTTCAGGGGGCAGTGCAGGCCAGAGCAGCATCTTCCAGAGTCTTGATGT CCTTCTGGGAATAAAACACGAGGCTGGCAAAGAATCTCCTGCAGAATTCCTCCAGGAAATGAGAGAGTAC ATGCCTCCAGCCCACCGGAACTTCCTTTTCTTCTTAGAGTCAGCTCCCCCAGTCCGTGAGTTTGTCATTT CAAGACACAATGAAGACTTGACGAAAGCTTATAACGAGTGTGTGAATGGTCTGGTCTCTGTGAGAAAGTT CCACCTCGCAATAGTAGATACTTACATTATGAAACCTTCGAAGAAGAAGCCCACTGATGGCGACAAGTCG GAAGAGCCCTCAAATGTGGAAAGCAGAGGGACTGGGGGTACGAATCCCATGACTTTCCTAAGGAGTGTGA AAGATACAACCGAGAAAGCTCTTCTGAGTTGGCCTTAGTGTAGCAAGCTCCACTTCTATCAGGGCACAGA AAACACCTTCATCCTGTCATAGCTCATTAAATCAGATCCACCAAGTAAGACTATAGAATAATTTGCCTGT TGTATATTATGTAGATTTCAAAAATCATCTGTGCATTCCCTGTAGGAAAATAATCAAGCTGAACTATTTA ATGATATTAAAATGATATAAGAAATAATATAAAATATATTTATGATTGAAATACAACAATGAGACCCAGT AAATAAAAGTTATTGTGAAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008324
• Insert Size: 1224 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC049931, AAH49931
• RefSeq Size: 1533 bp
• RefSeq ORF: 1224 bp
• Locus ID: 15930
• UniProt ID: P28776
• Cytogenetics: 8 A2
• Summary: Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longer isoform (1).