Chst2 (NM_018763) Mouse Untagged Clone

SKU MC202009

Chst2 (untagged) - Mouse carbohydrate sulfotransferase 2 (Chst2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Chst2
• Synonyms: AI428561; AW121776; C130041E03Rik; Chts2; GlcNAc6ST; Gn6st; GST-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051963 sequence for NM_018763; AGAGAAACCGAGGAGAGTGCTAGCCGGACAGTCCGCCGGTCGGGGATCTGGGGACGCTCCGAGGCGCACC CTCCGCTCCAGGTCCTTCTCGGAGCCGCTGCCATGGGAGAGCCAGCCCTGGGCGCCGGGGACCAGCAGCC TCTGCCGCCGCGCCCGCCTCGGATCGGCGGCCCCAGTCCCGGCGCCCGCAGCCGGCCTGCAGCGTCCCCC TCCTGGGCTGCAGGGCCGCCTCCGCCGCGCCGCCGGCCCCGGCTGTGCCTGTGATGAGCCGCAGCTCGCC GCGAGCTCTGCCCCCCGGTGCGCTTCCCCGGCCGCTGCCGGCCGCGCCTGCCGCCGTGCAGCGGGCCCTG CTCCCGCCGTGGCCCCGGCGCGCAGGACGCCGCTGGCCTGCGTCCCCGCTCGGGATGAAGGTATTTCGCA GGAAGGCGCTGGTGCTGTGCGCGGGCTATGCACTGCTACTGGTGCTCACGATGCTCAACCTCTTGGACTA CAAGTGGCATAAAGAGCCGCTGCAGCAGTGCAACCCCGACGGGCCTCTGGGTGCCGCGGTAGGGGCGGCC GGGGCCGGCTGGGGACGGCCGGGGTCGCCTCCTGCAGCGCCACCCCGCGCTCACTCTCGCATGGACCCCC GCACCCCGTACCGCCCTCCTGCCGCGGGCGTGGGGGCAGTTCCCGCAGCCGCGGCTGGGAGTGCAGGAGC TGCGGCCTCTCTGGGCAATGCTACTCGAGGCACCAGGGGTGGAGGGGACAAGCGGCAGTTGGTGTATGTG TTCACCACGTGGCGCTCGGGCTCGTCCTTCTTCGGTGAGCTCTTCAACCAGAACCCTGAGGTGTTCTTCC TCTATGAGCCTGTGTGGCACGTGTGGCAAAAACTGTACCCCGGGGACGCCGTTTCCCTGCAGGGGGCAGC GCGGGACATGCTGAGCGCTCTCTACCGCTGCGATCTTTCGGTTTTCCAGCTGTATAGCCCCGCAGGCAGT GGGGGGCGCAACCTCACCACTCTGGGCATCTTTGGGGCAGCCACTAACAAGGTGGTATGCTCCTCGCCAC TCTGTCCTGCCTACCGCAAGGAGGTCGTCGGACTGGTGGACGACCGCGTGTGCAAAAAGTGCCCACCTCA ACGCCTGGCACGCTTCGAGGAGGAGTGTCGCAAGTACCGCACGCTGGTTATCAAGGGCGTGCGGGTCTTC GATGTGGCTGTGTTGGCGCCGCTGCTTAAAGATCCAGCCTTGGACCTCAAGGTCATCCACCTAGTACGTG ATCCTCGTGCTGTTGCCAGCTCCCGCATCCGCTCGCGTCACGGCCTCATCCGGGAAAGCCTACAGGTGGT GCGAAGCCGGGATCCAAGAGCCCACCGCATGCCCTTCCTGGAGGCTGCTGGCCACAAGCTTGGTGCCAAG AAGGAGGGTATGGGTGGCCCAGCAGACTACCACGCTCTGGGTGCAATGGAGGTCATCTGCAACAGTATGG CCAAGACGCTGCAAACAGCCCTGCAGCCTCCTGACTGGCTGCAGGGACACTACTTGGTGGTGAGGTACGA GGATCTGGTGGGAGACCCCGTTAAGACCCTACGGAGGGTATATGACTTTGTGGGGCTGCTGGTGAGTCCC GAAATGGAGCAGTTTGCCCTGAACATGACCAGTGGTTCGGGCTCCTCCTCCAAGCCTTTCGTGGTGTCAG CTCGCAATGCCACTCAGGCCGCCAATGCCTGGCGGACCGCGCTCACCTTCCAGCAGATCAAACAGGTGGA GGAGTTTTGCTACCAGCCCATGGCCGTGCTGGGCTATGAGCGGGTTAACAGTCCTGAGGAGGTCAAAGAC CTCAGCAAGACCTTGCTCAGGAAGCCCCGGCTTTGAGAAGGGTTCCCAAGAGATCTGACACTCTCCGGAG ACACCCACAAAAAGGATGGTGTTGTGTTTAAACAAACACAGCCCAGACCCAAGCTGAGGAAGCCCACATA TTCTATTATAGATATATAATATAAATAACCACACAGGCACTTGCTGTCAACGTTTTGAGTCAGTGCATTT CAAGGAACAGCCCTCAACTCACACGTGCAAACACACACACACACACACACACACACACACACACACACAC ACACACACACACACCCCTCAGAAAAGGCAAGACTTGAAAGTTCTGACAGATTGCCCTGTGTCCCTGTCTC TTCTCCCTCTCTCCTTCCTCCCTCTTTCCCTCCTCCCTTCCATATTGAAGTGGAATGTTGATAAATCAAG TTCCAGTAACCCAAATCTTGTTTACAAAATTTTCGTGGTATCTGTGAATGTGTAAGAGTAATTTGGATGT GGGATGGGGTGGGTGGAGAAGGGGGAAGTGACCCCCAGAGCAGAAAGCCCCCACTGGGCTGGTAAATCAA GGAGGCAGGTCTTCAAAGTAGACTTTTGTGTCAGCAAAGGTTATATGTGAGTATTAATAAAGAAGATAAT AAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_018763
• Insert Size: 1452 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC051963, AAH51963
• RefSeq Size: 2501 bp
• RefSeq ORF: 1452 bp
• Locus ID: 54371
• UniProt ID: Q80WV3
• Cytogenetics: 9 E3.3
• Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.[UniProtKB/Swiss-Prot Function]