Dcp1a (NM_133761) Mouse Untagged Clone

SKU MC201914

Dcp1a (untagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dcp1a
• Synonyms: 1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; Mitc1; SMIF
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066173 sequence for NM_133761; CTTTGCCTGGATGGCACTTTCCTGTTCCACAGTCCGCCCCCGCCGGAGGGGCTCGGCTCTGAGATCCAAG ATGGAGTTGTTGAGTCGAGCTGAGCAAGAGATGAGTCTGGCGGCCTTGAAACAACACGACCCCTATATCA CCAGCATCGCAGACCTCACGGGCCAGGTCGCCCTGTACACTTTCTGCCCCAAAGCCAACCAGTGGGAGAA GACTGATATAGAAGGGACGTTATTTGTATACCGAAGGTCAGCTTCGCCTTACCATGGCTTTACCATTGTC AACCGACTGAACATGCACAATCTGGTTGAGCCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCAT TTCTTCTGTATAGAAATGCAAGTTTGTCAATATACAGTATCTGGTTCTATGACAAGAATGACTGTCATCG CATAGCAAAACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAGGCTGCTCGAGATAAA CAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCAGAGGCCCATAGATATCCTGGAGATGCTGAGCCGGG CCAAGGATGAGTATGAGAGGAATCAGATGGGCGGCTCAAATATCTCCAGTCCTGGGCTACAGCCAAGCAC TCAGCTCTCTAATCTGGGAAGCACCGAGACTCTAGAAGAGACACCCTCTGGGTCACAGGATAAGTCTGCT CCGTCTGGTCATAAACATCTGACAGTAGAAGAGTTATTTGGAACCTCCTTGCCAAAGGAACAACCAACAG CTATGGGTCTAGAGTCAGAGGACACAGACAAGCTGCTGGGAGATGCATCACAGAAAGAGCCCAGCTCATT CCTCCCATTTCCCTTTGAGCAGTCAGGGGGAGCCCCTCAGTCTGAAAACCTGGGTATCCATTCTGCCGCT CACCACACAGTTCAGCCTGAAGTCAGCACCCCAGTGCTGATCACTCCCGCCTCCATTGCACAGTCGGGTG ACAAACATCCCCCAAGCTACACGCTCCCACTGAGCCCTGTCCTCAGCCCCACTCTGCCAGCTGAAGCTCC TACCACACAAGTTCCCCACTTACCTCGGAATAGCACCATGATACAAGCAGTAAAGACCACACCCAGGCAG AAATCTCCACTCCTGAACCAGCCAGTCCCTGAGCTAAGCCACAGCAGTCTGGTTGCTAGCCAGAGCCCCT TCAGAGCCCCGGTGAGCCTAGCAAACCCAGCTGGCACAGCCCTCCCAAGCGTTGATCTGCTTCAGAAACT CAGGTTGACCCCACAGCATGACCAAATACAAGCACAGCCGCTTGGAAAAGGAACCATGGCGCCCAGCTTT TCTTCGGCAGCTGGCCAACTGGCCACCCCTGAGAGCTTCATAGAACCTTCTTCTAAGACAGCAGCAGCAA GAGCAGCAGTCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCAACCCTCCGGTCTATGCAGCAAAACCA GGATCCTGAAGTATTTTCCCAGCCTAAGGTGTTACCCAGTGCCATCCCGATTGCAGGCTCTCCATTGGTT CCTGCAACCACCACAGCAGTATCCTCAGTCCTGCTGTCCCCCAGTGTGTTCCAGCAAACGGTTCCCAGGG CCGCAGACCTAGAGAGGAAAGCCAGCTCCCCCTCTCCCCTCACTGTTGGGACAGCAGAGAGTCAGAGAAA GCCTTCCATTATCCTCAGCAAGTCTCAGCTCCAGGACACACTGATACACCTAATAAAGAATGACTCCAGC TTCCTCAGTACGCTTCATGCAGTTTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTATGAC TGCAGTGAAGGCAATGTGAAGGGAGAGGGTCCGCCTCAAGACACGCAGGCTTCCTCATGGAACAGCTAAG CAGAGCATGGAGCTTGAGGAGCCTGAGAGCCTTGTCCGTAAGGATGTTTCCTAGAGACGTTCTCAGTGAT GATGGAGGTTTCACTGTGCAGCATCACCAGCAAACCTTTGTTTGACAAAACAACAAGAGACCATTGTGTT GAGTGACGTGGGTGTTTTCATCAGCTACAAGCCAGTGGGTGGAATGAGGAGCCTGACTGCCGGCTTGCTT TCCAGGCCTCGGGAGGAGCCACCAGCCTTGCAGCAGAGCTCAGTGGAGCCCACGCCCTCCCTCCTCTACT CCTAGAAGAACTTAATGTCAGCTCACAGAGTAACTTCAGGTTTTAAGGATCAGAATTTAAGTTGACTCTT TGGAAGGTATTTGTTCAAGTGAAGTATATTTTCTATGAGCACCCCGCTTGGGCCTTAAGACTTCTGGGAT GTCTTTGTGTTCCGTAGTAGAGGCCGTAACTGCCATTGTAACACTGATAATGCAACATGAAACTCAAGGT GCCTACCTGCTCATACAGCGGAGTCGGCCATCATACTGCAACTCAGAGGGAAGAGAAGGAAGGGCACGGG CACAGGAGGACCCTGCGAGTCAGGCACTGAGGGGTCACAGCCTGCGTCGTCCTGTGTCCTCGTGGCACAC CTACTGAGGACCATGTGTCTCTTCCTGCCTGACTTTGTTGTTCTTCAGTACCAGCATTGCCCGCCCTGTT CAAGAATCCAGAATGCTCCGGTCTGAGCAGAGCACCTTACAGTGGGCTTTGAAGGCCACTCACGAGGAGA GTTTCCATGAGGACCAGGGCCTCCAGGAGCCTGTGGGGTTTAGGTTTGGGGTTGTTTTGTTGTTTTTTTA TTTCAAGTTTCAGCAGGAGAGATGTGCAATGTTTCAATAGGATTCCGATTTGACTTCACTCTTTCAAACG CTGCAATGCAATACAGATCACTTTGAAGTGGGGAAAAATGCAAAGTTGTGTTTCCCTTTTATCTGTGAAT CTTTAAGACACGTGTTGTTGCTGTACGTGCTGAGTGGTCTTATTCTCTACTGCTTCGGCGTGGGGCTCCC ATGACGTCATTGCACCGGGCTCTCAGCCCTTCCGGGGAGGCAAGAAGCCCCGCGCCACCTCGAGCGAAGC TTTTGTTCCGTGAGCGCAGTACCACCTCATTAACCTTTTTAGTGGTGTGGATTTTATACCATCTGTGTAT GTTTGCAAATATTAAGTTATTACATGTTTCAAAATGAGCATTTTTCATCCTAAATTTTATATGTTTGCAA ATATATTTTTTTAATAAAATTTCAAAACCAAGTTTTAGCACCTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133761
• Insert Size: 1809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC066173, AAH66173
• RefSeq Size: 3209 bp
• RefSeq ORF: 1809 bp
• Locus ID: 75901
• UniProt ID: Q91YD3
• Cytogenetics: 14 18.75 cM
• Summary: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity).[UniProtKB/Swiss-Prot Function]