Aadat (NM_011834) Mouse Untagged Clone

CAT#: MC200934

Aadat (untagged) - Mouse aminoadipate aminotransferase (Aadat), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aadat
• Synonyms: Aadt; AI875679; Kat2; KATII; Kyat2; mKat-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012637 sequence for NM_011834
  CCACGCGTCCGGGTGGCACTACACAGGGACAGCTAACAGTGCATCCCGAGTGACTGGCTTTCTGAATCCT GCTCCACGCGACCAGCAGAGACATGAATTACTCACGGTTCCTCACTGCAACGAGCCTGGCCAGAAAGCCA TCTCCCATCAGAACTACAGCGGACATACTGAGCAAAGCACCAAAAACCCTCATCTCCCTGGCTCCTGGAT CTCCAAACCCGAGCATGTTCCCCTTTAAGTCAGCTGCCTTCACTGTGGAAAACGGAAGCACCATCCGGTT TGAAGACGACTTGATCAAAAGGGCCCTCCAATACTCTCCAAGCTATGGAATTCCAGAACTTCTGTCCTGG CTAAAACAGTTTCAAGTAAAATTGCATAATCCCCCCACTGTCAACTACCCACCCAATCAAGGACAGATGG ATCTCTGCATCACATCTGGCTGCCAAGATGGTCTCTGTAAGGCATTTGAAATGCTCATCAATCCTGGAGA CACTATCCTGGTCAATGAACCACTGTTTCCAGGAACCCTTTATGCTATGAAACCACTAGGCTGCAACATT ATTAATGTCCCCAGTGATGAGCATGGGATTATTCCAGAGGGTCTCAAAAAAATCCTTTCCCAGTGGAAAC CAGAAGATTCCAAGGATCCCACAAAAAAGACTCCAAAATTTCTGTATACTGTCCCAAATGGCAACAACCC TACAGGCAACTCGTTGACAGGTGACCGCAAGAAGGAAATCTATGAGCTTGCAAGAAAATATGATTTCCTC ATAATAGAAGATGACCCTTACTATTTTCTTCAGTTTAGCAAGCCTTGGGAGCCAACTTTTCTCTCCATGG ATGTTGATGGGAGAGTCATCAGAGCTGACACCTTTTCAAAAACCGTCTCCTCAGGGTTGAGAGTAGGGTT TATGACTGGCCCTAAGACCTTGATACAGAATATTGTTCTCCACACACAAGTCTCATCAGTACATGCCTGT ACTCTCTCACAGCTCATGATACTACAGCTTCTACACCAATGGGGAGAAGAGGGTTTCCTGGCTCATATTG ACAGAACTATTGATTTCTACAAGAACCAGAGGGATTCCATATTGGCAGCTGCAGACAAGTGGTTACGTGG CTTGGCAGAGTGGCATGTTCCCAAAGCTGGCATGTTTCTATGGATTAAAGTTAAGGGAATCTCCGATACA AAACAACTGATTGAAGAAAAGGCTATTGAAAGAGAGGTCTTACTTGTTCCTGGAAATGGTTTCTTCATTG ATGGCTCAGCTCCTACCTCCTTCTTCAGAGCATCCTTCTCTCTGGCTACTCCAGCACAGATGGATACAGC CTTCCAGAGATTGGCCCAACTGATAAAAGAGTCTTTATGAAGAAATCAAACTCAGCATTGGACTCATAAT TTTTAAATAAATTACTTCTGTACTTTGCAGAAGAAATGGTTGTTGCTGTTTGACTGACAGGATGGATCCA GGTTGTGAAACATCTGTAGCAATTTCCCTGAACCACTTTAAAGTCCCCTTAAATCCATCACATTGCCAAA TAGCTTTCTGATCTACTTTTGCCCTTTGATTAATTTGCAACTGACAAAGCATTAAATTTTATTGTAAAGA GCTCTATAGCTGCTTTATAATAGCCAATAAATTTTTTTCAAAACTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011834
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012637, AAH12637
• RefSeq Size: 1670 bp
• RefSeq ORF: 1278 bp
• Locus ID: 23923
• UniProt ID: Q9WVM8
• Cytogenetics: 8 30.85 cM
• Gene Summary: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]