Tgfb1 (BC013738) Mouse Untagged Clone

SKU MC200640

Tgfb1 (untagged) - Mouse transforming growth factor, beta 1 (cDNA clone MGC:5747 IMAGE:3586216), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Tgfb1
• Synonyms: TGF-beta1; Tgfb; Tgfb-1; TGFbeta1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013738; GGACCCTCTCTCCCCCGAGAGGCAGATCTCCCTCGGACCTGCTGGCAGTAGCTCCCCTATTTAAGAACAC CCACTTTTGGATCTCAGAGAGCGCTCATCTCGATTTTTACCCTGGTGGTATACTGAGACACCTTGGTGTC AGAGCCTCACCGCGACTCCTGCTGCTTTCTCCCTCAACCTCAAATTATTCAGGACTATCACCTACCTTTC CTTGGGAGACCCCACCCCACAAGCCCTGCAGGGGCGGGGCCTCCGCATCCCACCTTTGCCGAGGGTTCCC GCTCTCCGAAGTGCCGTGGGGCGCCGCCTCCCCCATGCCGCCCTCGGGGCTGCGGCTACTGCCGCTTCTG CTCCCACTCCCGTGGCTTCTAGTGCTGACGCCCGGGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCA TCGACATGGAGCTGGTGAAACGGAAGCGCATCGAAGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCT CGCCAGTCCCCCAAGCCAGGGGGAGGTACCGCCCGGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAAC AGCACCCGCGACCGGGTGGCAGGCGAGAGCGCCGACCCAGAGCCGGAGCCCGAAGCGGACTACTATGCTA AAGAGGTCACCCGCGTGCTAATGGTGGACCGCAACAACGCCATCTATGAGAAAACCAAAGACATCTCACA CAGTATATATATGTTCTTCAATACGTCAGACATTCGGGAAGCAGTGCCCGAACCCCCATTGCTGTCCCGT GCAGAGCTGCGCTTGCAGAGATTAAAATCAAGTGTGGAGCAACATGTGGAACTCTACCAGAAATATAGCA ACAATTCCTGGCGTTACCTTGGTAACCGGCTGCTGACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGA CGTCACTGGAGTTGTACGGCAGTGGCTGAACCAAGGAGACGGAATACAGGGCTTTCGATTCAGCGCTCAC TGCTCTTGTGACAGCAAAGATAACAAACTCCACGTGGAAATCAACGGGATCAGCCCCAAACGTCGGGGCG ACCTGGGCACCATCCATGACATGAACCGGCCCTTCCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCCCA GCACCTGCACAGCTCACGGCACCGGAGAGCCCTGGATACCAACTATTGCTTCAGCTCCACAGAGAAGAAC TGCTGTGTGCGGCAGCTGTACATTGACTTTAGGAAGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGG GCTACCATGCCAACTTCTGTCTGGGACCCTGCCCCTATATTTGGAGCCTGGACACACAGTACAGCAAGGT CCTTGCCCTCTACAACCAACACAACCCGGGCGCTTCGGCGTCACCGTGCTGCGTGCCGCAGGCTTTGGAG CCACTGCCCATCGTCTACTACGTGGGTCGCAAGCCCAAGGTGGAGCAGTTGTCCAACATGATTGTGCGCT CCTGCAAGTGCAGCTGAAGCCCCGCCCCGCCCCGCCCCTCCCGGCAGGCCCGGCCCCGCCCCCGCCCCGC CCGCAGCGCCCGGGGCTGTATTTAAGGACACCTGCACCCCCCCTCAAGCCCACCTGGGGGGCCCATTAAA GGTGACAGAGGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC013738
• Insert Size: 1173 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC013738, AAH13738
• RefSeq Size: 1641 bp
• RefSeq ORF: 1173 bp
• Locus ID: 21803
• Cytogenetics: 7 13.98 cM
• Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016]