Vapa (NM_013933) Mouse Untagged Clone

CAT#: MC200063

Vapa (untagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vapa
• Synonyms: 33kDa; VAP33
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003866 sequence for NM_013933
  GGATCGCAGTGCGCGTGGCCGCGGCGGCTGGTGTGGGGTTGAGTCAGTTGTGACACCCGGAGCTGCTGAG CGGACAGGCTGCCCTCGGGACCCCCGCGGCCCGGCAGCAGGCGCGGAGCTTCGGGAATCGTGAGCCTGGC CCCGTCCTAGAGCTCCGCGGAGCCGGCGCCGCCACCGTCCCCTCGCCCCCGCCAGCGAACCGCCGCCGCG GGCGCGCCCCCGCTCTGCGCTGCGCTGTCTCCTATGGCGTCCGCCTCCGGGGCCATGGCGAAGCACGAGC AGATCCTGGTCCTCGACCCTCCTTCAGACCTCAAATTCAAAGGCCCCTTCACAGATGTAGTCACTACAAA TCTTAAATTGCAAAATCCATCGGATAGAAAAGTGTGTTTCAAAGTGAAGACTACAGCACCTCGCCGGTAC TGTGTGCGGCCCAACAGTGGGATTATTGACCCGGGGTCAATTGTGACTGTTTCAGTAATGCTGCAACCCT TTGATTATGATCCGAATGAAAAGAGTAAACATAAGTTCATGGTACAGACAATTTTTGCTCCACCAAACAT TTCAGATATGGAAGCTGTGTGGAAAGAAGCAAAACCTGATGAATTAATGGATTCTAAATTGAGATGTGTG TTTGAAATGCCGAATGAAAATGATAAGCTGAATGATATGGAACCTAGCAAAGCTGTTCCACTGAATGCAT CCAAACAAGACGGACCCCTGCCAAAACCACACAGTGTTTCACTCAATGATACGGAAACAAGGAAACTGAT GGAAGAGTGCAAGCGACTCCAGGGAGAAATGATGAAGCTCTCAGAAGAAAACCGACACCTGAGAGATGAA GGCCTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCCACCTCAGCCGTGTCCTTCAGAGATA ATGTCACCAGTCCTCTTCCTTCTCTTCTGGTTGTAATTGCAGCCATTTTCATTGGATTCTTTCTAGGGAA ATTCATCTTGTAGAGTGAAGCATGCAGAGTGCTGTTTCTTTTTTTTTTTTTTTCTTCTCTCGACCAGAAA AAGATTTGTTTACCTACCATTTCATTGGTAGTATGGCCCGCGGTGACCATGGCCCGCGGTGACCAATTTT TTTTGTGTGTACAGCGTCATATAGGCTTTGCCTTTAATGATCTCTTACGGTTAGAAAACACAATAAAAAC AAACTGTTCGGCTACTGGACAAATTGTATATTATCAGATCATCACTAGCAGATGTCAGTTGCACATTCAG TCCTTTATGAAATTCATAAATAAAGAATTGTTCTTTCTTTGTGGTTTTAATAAGAGTTCAAGAATTGTTC AGTCTTGTAAATGTTATTTTAATAATTCCTTTAAATTTTATCTGTTGCTGTTACCTCTTGAAATATGATT TATTTAGATTGCTAATCCCACTCATTCAGGAAATGCCAAGAGGTTTTCTGTGGGGGAAATGGTGCCTCTT ACTGTGTAAATTTTCTCCTTTACCTTTGCTAATATCATGGCAGAATTTTTCTTATCCCTTGTGAGGCAGT TTGTTGACTGAGTTTTTCATCCTTACAATCCTGTCCCATGGTATTTAACATAAAAAAAATAAAACTGTTA ACAGATTCTTGCTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013933
• Insert Size: 750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003866, AAH03866
• RefSeq Size: 1668 bp
• RefSeq ORF: 750 bp
• Locus ID: 30960
• UniProt ID: Q9WV55
• Cytogenetics: 17 E1.1
• Gene Summary: Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function]