Rat Wls (NM_001085353) AAV Particle

SKU RR216879A1V

AAV ORF Particles, serotype AAV-2, Wls (myc-DDK-tagged) - Rat wntless Wnt ligand secretion mediator (Wls), transcript variant 2, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Wls
• Synonyms: Gpr177
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216879 representing NM_001085353; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGGGGCAATTATAGAAAACATGAGCACCAAGAAGCTCTGCATTGTTGGAGGGATTCTTCTGGTTT; TCCAAATCGTTGCCTTTCTGGTGGGAGGCTTGATCGCTCCAGCACCCACAACGGCAGTGTCCTACGTGGC; AGCAAAATGTGTGGATGTCCGGAAGAACCACCATAAAACAAGATGGCTGATGCCCTGGGGACCAAACAAG; TGTAACAAGATCAATGACTTCGAAGAAGCAATTCCAAGGGAAATTGAAGCGAATGACATTGTGTTTTCTG; TACACATTCCCCTCCCTTCTATGGAGATGAGCCCATGGTTCCAGTTTATGCTGTTTATCCTGCAGATAGA; CATTGCTTTCAAGCTAAACAACCAAATCAGAGAAAATGCAGAAGTTTCCATGGATGTTTCCCTGGGTTAC; CGTGATGATATGTTTTCTGAGTGGACTGAAATGGCGCACGAAAGAGTACCACGTAAACTCAGATGCACTT; TCACATCCCCCAAGACCCCAGAGCATGAAGGTCGTCATTATGAATGTGATGTCCTTCCTTTCATGGAAAT; TGGGTCAGTGGCTCATAAGTATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAGAAGAAAATCAAT; GTTGGAATTGGGGAAATAAAGGACATTCGGTTGGTGGGAATCCACCAAAATGGAGGTTTCACTAAGGTAT; GGTTTGCTATGAAGACCTTCCTCACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGAAGGATCAC; CATGATGTCCCGACCTCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATC; AATATCCCTGTGGAATGGTTTTCCATTGGATTTGATTGGACCTGGATGCTGTTATTTGGTGACATACGAC; AGGGCATCTTCTATGCAATGCTTCTTTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACA; TGAGCGGAATCACATTGCAGGGTATTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCTTTCTGCCTCTTC; ATATTTGACATGTGTGAGAGAGGAGTGCAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTG; GAACAGAACTGGCTATGGCTTTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTACTTCCTGTTTCTGTG; TTTCATGGTATTTCAAGTATTCAGAAACATCAGTGGGAAACAGTCTAGCCTCCCAGCCATGAGCAAAGTC; CGGAGGCTGCACTATGAGGGTCTGATTTTCAGGTTCAAGTTCCTCATGCTGATCACCTTGGCTTGTGCTG; CCATGACTGTTATCTTCTTCATTGTTAGTCAGGTGACAGAAGGCCATTGGAAATGGGGTGGGGTCACAGT; TCAAGTGAGCAGTGCTTTCTTCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTC; TTGTATGCACCATCCCATAAGAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGG; AAGAACTGCAGCTCACTACCACAATCACCCATGTAGATGGACCGACTGAGATCTACAAGTTGACCCGTAA; AGAAGCACAGGAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216879 representing NM_001085353; Red=Cloning site Green=Tags(s); ; MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVSYVAAKCVDVRKNHHKTRWLMPWGPNK; CNKINDFEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQIDIAFKLNNQIRENAEVSMDVSLGY; RDDMFSEWTEMAHERVPRKLRCTFTSPKTPEHEGRHYECDVLPFMEIGSVAHKYYLLNIRLPVNEKKKIN; VGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFI; NIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLF; IFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKV; RRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMF; LYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Rat
• Serotype: AAV-2
• ACCN: NM_001085353
• ORF Size: 1623 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001085353.1, NP_001078822.1
• RefSeq Size: 2640 bp
• RefSeq ORF: 1626 bp
• Locus ID: 362065
• UniProt ID: Q6P689
• Cytogenetics: 2q45
• MW: 62.2 kDa