Human TIE1 (NM_005424) AAV Particle

SKU RC600056A1V

AAV ORF Particle, Serotype AAV-2, TIE1 (Myc-DDK-tagged)-Extra Cellular Domain Clone of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant 1, Signal peptide (1-21) plus EC domain (22-759), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: TIE1
• Synonyms: JTK14; LMPHM11; TIE
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600056 representing leader sequence plus the extracellular domain region of NM_005424 ; Red=Cloning site Blue=ORF Green=Tags(s); ; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG; CGATCGCC; ; ATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATGTGGGCGCGGCGGTGG; ACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCTTCTTCCTGACTTGCGTGTCTGGGGA; GGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTG; CGCACCCCGCCCGGGCCACCCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCT; CCAAGCCCTCGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCAT; CTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAGGTGAC; ACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGCAACGGATCCT; ACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTGCAGCTCCCAAATGTGCAGCC; ACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTC; ATCGTGCGGGGTTGTGGGGCTGGGCGCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATG; GAGGTGTCTGCCACGACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACA; GGCCTGCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCCGGGGC; CTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAAG; AAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAGTGTCAGAATGGTGGCACTTG; TGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCATGGAGTGCACTGTGAGAAGTCAGACCGGATC; CCCCAGATCCTCAACATGGCCTCAGAACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAG; CTGCAGGGAACCCCTTCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTC; CACCAAGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTGCGGAC; AGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTGA; AAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAGAGCCGCCAGCTTGTGGTCTCCCC; GCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTCCGCCTGCACTACCGGCCCCAGGACAGTACC; ATGGACTGGTCGACCATTGTGGTGGACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAG; GATACAGTGTTCGTGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCT; CATGACCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCACTGAC; CGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACGGTTTCCTGCTGCGCC; TGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCATCCCCCCAGGCCCGCACTGCCCTCCT; GACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCG; GCCTCGCCCCCTGCACACGTGCTTCTGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGG; CCCTCTCAGACTCCGAGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTA; CGTTGTGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTGAGGAG; ACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGCGGGCCAGCATTCAGG; GGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCAACGGGCTGCAGGCTGAGGGCCCAGT; CCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATCAG; ; ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC; TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600056 representing signal peptide plus the extracellular domain region of NM_005424; Red=Cloning sites Green= DDK and 6XHIS Tags; ; MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIV; RTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGD; TAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRL; IVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRG; LTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRI; PQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLAD; SGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDST; MDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTD; RLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGP; ASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEE; TSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQ; ; TRSGTRSGDYKDDDDKGSHHHHHH
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005424
• ORF Size: 2277 bp
• Buffer: PBS with 0.001% Pluronic F11418
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005424.4, NP_005415.1
• RefSeq Size: 4000 bp
• RefSeq ORF: 3417 bp
• Locus ID: 7075
• UniProt ID: P35590
• Cytogenetics: 1p34.2
• MW: 82.2 kDa