Human NIT1 (NM_001185093) AAV Particle

SKU RC231154A1V

AAV ORF Particles, serotype AAV-2, NIT1 (Myc-DDK-tagged)-Human nitrilase 1 (NIT1), transcript variant 3, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: NIT1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231154 representing NM_001185093; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACTTTTGGACTGCGGAAACGTTTGTCAGAGGAAAGAGGCTTCAGTTTAATGCCCAGAGCCATGGCTA; TCTCCTCTTCCTCCTGCGAACTGCCCCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAGCAACA; GAACTTTAAAACATGTGCTGAGCTGGTTCGAGAGGCTGCCAGACTGGGTGCCTGCCTGGCTTTCCTGCCT; GAGGCATTTGACTTCATTGCACGGGACCCTGCAGAGACGCTACACCTGTCTGAACCACTGGGTGGGAAAC; TTTTGGAAGAATACACCCAGCTTGCCAGGGAATGTGGACTCTGGCTGTCCTTGGGTGGTTTCCATGAGCG; TGGCCAAGACTGGGAGCAGACTCAGAAAATCTACAATTGTCACGTGCTGCTGAACAGCAAAGGGGCAGTA; GTGGCCACTTACAGGAAGACACATCTGTGTGACGTAGAGATTCCAGGGCAGGGGCCTATGTGTGAAAGCA; ACTCTACCATGCCTGGGCCCAGTCTTGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTAGCTGT; CTGCTATGACATGCGGTTCCCTGAACTCTCTCTGGCATTGGCTCAAGCTGGAGCAGAGATACTTACCTAT; CCTTCAGCTTTTGGATCCATTACAGGCCCAGCCCACTGGGAGGTGTTGCTGCGGGCCCGTGCTATCGAAA; CCCAGTGCTATGTAGTGGCAGCAGCACAGTGTGGACGCCACCATGAGAAGAGAGCAAGTTATGGCCACAG; CATGGTGGTAGACCCCTGGGGAACAGTGGTGGCCCGCTGCTCTGAGGGGCCAGGCCTCTGCCTTGCCCGA; ATAGACCTCAACTATCTGCGACAGTTGCGCCGACACCTGCCTGTGTTCCAGCACCGCAGGCCTGACCTCT; ATGGCAATCTGGGTCACCCACTGTCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231154 representing NM_001185093; Red=Cloning site Green=Tags(s); ; MTFGLRKRLSEERGFSLMPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLP; EAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAV; VATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTY; PSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLAR; IDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001185093
• ORF Size: 936 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001185093.1, NP_001172022.1
• RefSeq ORF: 939 bp
• Locus ID: 4817
• UniProt ID: Q86X76
• Cytogenetics: 1q23.3
• MW: 34.8 kDa