Human BRIT1 (MCPH1) (NM_001172574) AAV Particle

SKU RC230342A1V

AAV ORF Particle, Serotype AAV-2, MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 2, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: MCPH1
• Synonyms: BRIT1; MCT
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230342 representing NM_001172574; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCCCCCATCCTGAAAGATGTAGTGGCCTATGTTGAAGTGTGGTCATCCAATGGAACAGAAAATT; ATTCAAAGACATTTACAACACAGCTTGTGGATATGGGGGCAAAGGTTTCAAAAACTTTTAACAAACAAGT; AACTCACGTTATCTTCAAAGATGGCTACCAGAGCACTTGGGACAAAGCTCAGAAGAGAGGCGTAAAGCTC; GTTTCGGTGCTCTGGGTTGAAAAATGCAGGACAGCTGGAGCACACATTGATGAATCATTGTTCCCTGCAG; CTAATATGAATGAACACTTATCAAGCCTAATTAAAAAAAAACGTAAATGTATGCAGCCCAAGGATTTTAA; TTTTAAAACACCAGAAAATGATAAGAGATTTCAGAAGAAATTTGAGAAAATGGCTAAAGAGCTACAAAGG; CAAAAAACAAATCTAGATGATGATGTACCTATTCTCTTATTTGAATCTAATGGTTCATTAATATATACTC; CCACAATTGAAATTAATAGTAGTCACCACAGCGCAATGGAGAAGAGATTACAAGAGATGAAGGAGAAAAG; GGAAAATCTTTCCCCCACCTCTTCCCAAATGATTCAGCAGTCTCATGATAATCCAAGTAACTCTCTGTGT; GAAGCACCTTTGAACATTTCACGTGATACTTTGTGTTCAGATGAATACTTTGCTGGTGGCTTACACTCAT; CTTTTGATGATCTTTGTGGAAACTCAGGATGTGGAAATCAGGAAAGGAAGTTGGAAGGATCCATTAATGA; CATTAAAAGTGATGTGTGTATTTCTTCACTTGTATTGAAAGCAAATAATATTCATTCATCACCATCTTTC; ACTCACCTCGATAAATCAAGTCCTCAGAAATTTCTGAGTAATCTTTCAAAGGAAGAAATAAACTTGCAAA; GAAATATTGCAGGTAAAGTAGTCACCCCTCACCAAAAGCAGGCTGCAGGTATGTCTCAGGAGACGTTTGA; AGAGAAGTATCGTTTGTCTCCTACCTTATCTTCAACAAAAGGCCACCTTTTGATACATTCAAGACCCAGG; AGTTCCTCAGTAAAGAGAAAAAGAGTATCACATGGCTCCCATTCACCTCCGAAGGAAAAATGCAAGAGAA; AGAGGAGCACCAGGAGATCTATCATGCCGAGGCTGCAGCTGTGCAGGTCGGAAGGCAGGCTGCAGCACGT; GGCGGGACCTGCCCTGGAGGCTCTTAGCTGTGGGGAGTCTTCATATGATGACTATTTTTCACCTGATAAT; CTTAAGGAAAGGTATTCAGAGAATCTTCCTCCTGAATCTCAGCTGCCATCAAGCCCTGCTCAGTTGAGCT; GCAGAAGTCTTTCTAAGAAGGAGAGAACAAGCATATTTGAAATGTCTGATTTTTCCTGCGTTGGCAAAAA; AACCAGAACAGTTGACATTACCAATTTCACAGCAAAAACCATCTCCAGTCCTCGGAAAACTGGAAATGGT; GAAGGCCGTGCAACTTCGAGTTGCGTGACTTCTGCCCCTGAAGAAGCCCTAAGGTGTTGTAGACAGGCTG; GGAAAGAAGACGCATGCCCAGAGGGAAATGGCTTTTCTTACACCATTGAGGACCCTGCTCTTCCAAAAGG; ACATGATGATGATTTAACTCCTTTGGAAGGAAGCCTTGAAGAAATGAAAGAAGCGGTTGGTCTGAAAAGC; ACACAGAACAAAGGTACCACTTCCAAAATATCAAACTCCTCTGAAGGCGAAGCCCAGAGTGAACATGAGC; CATGTTTTATAGTTGACTGTAACATGGAGACGTCTACAGAAGAGAAGGAAAACTTACCCGGAGGATACAG; TGGAAGTATG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230342 representing NM_001172574; Red=Cloning site Green=Tags(s); ; MAAPILKDVVAYVEVWSSNGTENYSKTFTTQLVDMGAKVSKTFNKQVTHVIFKDGYQSTWDKAQKRGVKL; VSVLWVEKCRTAGAHIDESLFPAANMNEHLSSLIKKKRKCMQPKDFNFKTPENDKRFQKKFEKMAKELQR; QKTNLDDDVPILLFESNGSLIYTPTIEINSSHHSAMEKRLQEMKEKRENLSPTSSQMIQQSHDNPSNSLC; EAPLNISRDTLCSDEYFAGGLHSSFDDLCGNSGCGNQERKLEGSINDIKSDVCISSLVLKANNIHSSPSF; THLDKSSPQKFLSNLSKEEINLQRNIAGKVVTPHQKQAAGMSQETFEEKYRLSPTLSSTKGHLLIHSRPR; SSSVKRKRVSHGSHSPPKEKCKRKRSTRRSIMPRLQLCRSEGRLQHVAGPALEALSCGESSYDDYFSPDN; LKERYSENLPPESQLPSSPAQLSCRSLSKKERTSIFEMSDFSCVGKKTRTVDITNFTAKTISSPRKTGNG; EGRATSSCVTSAPEEALRCCRQAGKEDACPEGNGFSYTIEDPALPKGHDDDLTPLEGSLEEMKEAVGLKS; TQNKGTTSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKENLPGGYSGSM; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001172574
• ORF Size: 1830 bp
• Buffer: PBS with 0.001% Pluronic F9700
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001172574.1, NP_001166045.1
• RefSeq ORF: 1833 bp
• Locus ID: 79648
• UniProt ID: Q8NEM0
• Cytogenetics: 8p23.1
• MW: 68.3 kDa