Human RASGRP3 (NM_001139488) AAV Particle

SKU RC227703A1V

AAV ORF Particle, Serotype AAV-2, RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: RASGRP3
• Synonyms: GRP3
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227703 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTTG; ATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTATC; TTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAATTT; CGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGATCTTG; GTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTCAGCCT; CATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTATCCAAG; AAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACTTTTCTGG; AGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGCCTGGAGAA; TAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTGATGGTTCTT; AGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAGAAGCTCCTTC; AGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATTTCACGCCTCAA; AGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAGTTGGTCTCCTCC; AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATCCCCATCCTTGGAG; TACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAGAACAAAGTGAACAT; TGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAATGCCTCTCACCACTTA; GAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCACACTGAAGATGATATTT; ACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCAGCCTACCTCCCCTACGACGCCCAACAA; GCCTGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCCACGGTCATCAACAAGCAC; ATAAGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGATGGGTACATTTCCCAAGAGG; ACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTTCTGGACAAAGATCAGGATGG; CCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCCCAACTACACTGTAAAATGGGA; CCAGGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACCTTCTGCGAACACTGTGCGGGAT; TTCTCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGAGCCAATTGTCACAAACAGTGCAA; AGACCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCCTTGAGCAGTGGTCATGGGTCACTG; CCTGGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAGTTCCCTGGAGTCACTGCTGGACACA; GGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCTCGCAAGATCTCTGTGAGGCTACAGAG; GGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCAGAGGCTGGCTGGGGGGACTCGGGGTCC; CACACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCAGCAAAGGACAAAGGCTTTGCCAAATGGG; AAAATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTAGACCGGGGCACGGAGTTTGAACTTGACCA; GGATGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227703 protein sequence; Red=Cloning site Green=Tags(s); ; MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYRNATGESCNEF; RLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSK; KGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVL; SKPTPQQRAEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSS; NGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHL; EPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWALGVMPKPDPTVINKH; IRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMG; PGFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSL; PGSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGS; HTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001139488
• ORF Size: 2070 bp
• Buffer: PBS with 0.001% Pluronic F9342
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001139488.1
• RefSeq Size: 4798 bp
• RefSeq ORF: 2073 bp
• Locus ID: 25780
• UniProt ID: Q8IV61
• Cytogenetics: 2p22.3
• MW: 78.3 kDa