Human SPG20 (NM_001142295) AAV Particle

SKU RC227162A1V

AAV ORF Particle, Serotype AAV-2, SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 3, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SPG20
• Synonyms: SPG20; TAHCCP1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227162 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATATAAGAAGGCCT; TTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAA; GCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTACTGGG; TGGGAATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTGGAAA; TTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCAGAATT; TCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCATGCTGAA; GTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCTTTACCATCAC; AAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTA; TGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCT; CTTGAGACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATTTTTTTTGTAA; ATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTTTTGGATAATTC; TCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATATCCTCTAGTTCCT; GATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATGCTACAAGCAGCAG; GATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGAGAGCTCTTTGAGGATCTGTT; AAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAAAATGAATTCCAAATC; CCTGGAAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTTGGACC; AAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGATACTTCAAGTGAAGAAGT; TAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAAGAATTACATGAATGGAGT; GAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGTTTAGTCAAAGGTGCTGAGA; TTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATTCAACCAGAAGAAAAACCCGT; GGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAAAGTC; AGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACATGTCA; AGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAAATCTCCTCTGGATGG; TGCTATGGTTGTAGCAGCGAGTAGTGTTCAAGGATTTTCAACTGTCTGGCAAGGATTGGAATGTGCAGCT; AAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTCAGATACAAATACGGATATAATGCAG; GAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACAT; TGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCAGATA; GTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGATGAGC; AGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227162 protein sequence; Red=Cloning site Green=Tags(s); ; MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGTG; WESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAE; VNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPP; LETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVP; DRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQI; PGRTRPSSDQLKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELHEWS; EKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIAKQATGGAAKV; SQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMVVAASSVQGFSTVWQGLECAA; KCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVTAYNINNIGIKAMVKKTATQTGHTLLEDYQI; VDNSQRENQEGAANVNVRGEKDEQTKEVKEAKKKDK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001142295
• ORF Size: 1998 bp
• Buffer: PBS with 0.001% Pluronic F9248
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001142295.1, NP_001135767.1
• RefSeq Size: 4962 bp
• RefSeq ORF: 2001 bp
• Locus ID: 23111
• UniProt ID: Q8N0X7
• Cytogenetics: 13q13.3
• MW: 72.9 kDa