Human Apolipoprotein L 1 (APOL1) (NM_001136541) AAV Particle

SKU RC226644A1V

AAV ORF Particles, serotype AAV-2, APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 4, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Apolipoprotein L 1
• Synonyms: APO-L; APOL; APOL-I; FSGS4
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226644 representing NM_001136541; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGGTGCAACAAAACGTTCCAAGTGGGA; CAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATGGACCCAGAGAGCAG; TATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGCACACAGAATCTGCTACTCCTGCTG; ACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCTGCTGAACTGCCCAGGAATGAGGCAGATGAGCTCC; GTAAAGCTCTGGACAACCTTGCAAGACAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCA; GTACAGAAACTGGTTTCTGAAAGAGTTTCCTCGGTTGAAAAGTGAGCTTGAGGATAACATAAGAAGGCTC; CGTGCCCTTGCAGATGGGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGCTCTC; TCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACAGAGGGAGGCAGCCT; TGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAGCCGCTTTGACCGGGATTACCAGCAGTACCATG; GACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCCCACGACCTGGTCATCAAAAGCCTTGACAAATTGA; AGGAGGTGAGGGAGTTTTTGGGTGAGAACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACT; CACACGAGGCATTGGGAAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCAT; GCCTCAGCCTCACGCCCCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGGGTTA; ATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCTGTAAGCTTCTTTCT; TGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGCACTTACATGAGGGGGCAAAGTCAGAGACAGCT; GAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAGAAGCTAAACATTCTCAACAATAATTATAAGATTC; TGCAGGCGGACCAAGAACTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226644 representing NM_001136541; Red=Cloning site Green=Tags(s); ; MEGAALLRVSVLCIWVQQNVPSGTDTGDPQSKPLGDWAAGTMDPESSIFIEDAIKYFKEKVSTQNLLLLL; TDNEAWNGFVAAAELPRNEADELRKALDNLARQMIMKDKNWHDKGQQYRNWFLKEFPRLKSELEDNIRRL; RALADGVQKVHKGTTIANVVSGSLSISSGILTLVGMGLAPFTEGGSLVLLEPGMELGITAALTGITSSTM; DYGKKWWTQAQAHDLVIKSLDKLKEVREFLGENISNFLSLAGNTYQLTRGIGKDIRALRRARANLQSVPH; ASASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVVYLVYESKHLHEGAKSETA; EELKKVAQELEEKLNILNNNYKILQADQEL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001136541
• ORF Size: 1140 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001136541.1, NP_001130013.1
• RefSeq ORF: 1143 bp
• Locus ID: 8542
• UniProt ID: O14791
• Cytogenetics: 22q12.3
• MW: 42 kDa