Human VPS53 (NM_001128159) AAV Particle

SKU RC226200A1V

AAV ORF Particle, Serotype AAV-2, VPS53 (Myc-DDK-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: VPS53
• Synonyms: HCCS1; hVps53L; PCH2E; pp13624
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226200 representing NM_001128159.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCGTGCTGCAGCTCACGCCCGAGGTG; CAGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGCTGTTGAG; TATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGTCGTGAACAAAATTAGG; CTGAAAATAAGGAGACTGGATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGAT; GGACGGCAAGCGCTTGAAGAGGCTCAGAAAGCTATCCAACAACTCTTTGGCAAAATCAAAGATATCAAA; GACAAAGCTGAAAAATCAGAGCAAATGGTGAAAGAAATCACCCGTGATATTAAGCAATTAGATCACGCC; AAACGCCACCTGACCACCTCAATCACCACACTGAACCACCTGCACATGCTGGCAGGAGGTGTCGACTCC; CTCGAAGCCATGACCAGGCGAAGACAATACGGAGAAGTTGCTAATCTCCTTCAGGGTGTGATGAATGTC; CTGGAGCACTTCCACAAGTATATGGGGATTCCGCAGATCCGGCAGCTTTCCGAAAGAGTGAAGGCTGCA; CAGACTGAGTTAGGACAGCAAATCCTGGCAGATTTTGAAGAAGCGTTTCCTTCCCAGGGCACCAAGAGA; CCAGGAGGACCCAGCAATGTTCTACGAGATGCATGTCTGGTTGCTAATATTCTAGATCCCAGGATCAAA; CAGGAAATCATCAAAAAGTTTATTAAACAGCATCTGTCAGAGTATCTGGTACTTTTTCAAGAAAACCAA; GATGTTGCCTGGCTGGACAAAATCGACAGACGCTATGCCTGGATAAAACGCCAGCTTGTGGACTATGAG; GAGAAATACGGCCGCATGTTTCCACGTGAGTGGTGCATGGCTGAGAGGATTGCGGTGGAATTTTGCCAT; GTGACAAGGGCAGAACTTGCCAAGATTATGCGTACCAGAGCGAAGGAAATTGAAGTGAAATTGCTTCTT; TTTGCTATTCAAAGAACAACTAACTTTGAGGGGTTTCTTGCAAAACGCTTCTCCGGCTGCACCCTGACC; GATGGGACCCTGAAAAAGCTTGAGTCTCCACCCCCATCTACCAATCCCTTCCTGGAAGATGAGCCAACA; CCAGAGATGGAGGAACTGGCAACGGAGAAAGGAGATTTAGATCAACCAAAGAAGCCTAAAGCCCCAGAC; AATCCATTTCATGGCATTGTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTATATCGAATCCCAAGAC; AAGAACCTCGGAGAGCTGATAGATCGGTTTGTGGCTGATTTCAAAGCCCAGGGGCCACCTAAGCCCAAC; ACTGATGAAGGGGGTGCCGTGCTCCCCAGCTGCGCCGACCTCTTTGTCTACTACAAGAAGTGCATGGTG; CAATGCTCTCAGCTCAGTACTGGGGAGCCCATGATCGCCCTGACCACCATTTTCCAGAAGTACCTCCGA; GAATACGCCTGGAAAATCCTCTCTGGCAACCTGCCCAAAACCACAACCAGCAGTGGAGGACTGACTATC; AGCAGCCTCCTCAAGGAAAAGGAGGGCTCAGAAGTAGCCAAGTTCACTCTGGAGGAGCTCTGCCTCATC; TGTAACATCCTGAGCACGGCAGAGTACTGTCTGGCCACCACCCAGCAGCTAGAAGAAAAACTCAAAGAA; AAAGTGGATGTAAGTCTGATTGAACGAATCAATCTGACTGGAGAGATGGACACGTTCAGCACCGTCATC; TCCAGCAGTATTCAGCTGCTGGTTCAGGATCTGGATGCTGCCTGTGATCCTGCCCTGACTGCCATGAGC; AAGATGCAGTGGCAGAACGTGGAGCACGTTGGTGACCAGAGCCCCTACGTCACCTCTGTCATTCTGCAC; ATCAAGCAGAACGTCCCCATCATCCGTGACAACCTGGCTTCCACACGCAAGTACTTCACTCAGTTCTGC; GTTAAATTTGCAAACTCCTTCATTCCCAAATTCATCACCCACCTCTTCAAGTGCAAGCCAATTAGCATG; GTGGGAGCAGAACAGCTGCTGCTGGACACCCACTCGCTGAAGATGGTCCTGCTCGATCTCCCCTCCATC; AGCTCGCAGGTGGTGAGGAAGGCACCCGCCAGCTACACCAAGATCGTTGTCAAAGGCATGACCCGGGCT; GAGATGATCCTCAAGGTAGTGATGGCCCCTCATGAACCGTTGGTGGTGTTTGTTGACAACTACATCAAA; CTTCTCACAGACTGCAACACAGAAACCTTTCAGAAGATACTGGACATGAAGGGGCTGAAGAGGAGTGAG; CAGAGCAGCATGCTGGAACTCCTGCGCCAGCGGCTCCCCGCACCGCCCTCGGGGGCAGAAAGCTCCGGC; TCACTGTCCCTGACGGCGCCAACACCAGAGCAAGAGTCGTCACGCATCCGCAAGCTCGAGAAACTCATT; AAAAAGAGACTG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
  Protein Sequence

  >Peptide sequence encoded by RC226200; Blue=ORF Red=Cloning site Green=Tag(s); ; MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIR; LKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHA; KRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAA; QTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQ; DVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLL; FAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPD; NPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMV; QCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLI; CNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMS; KMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISM; VGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIK; LLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLI; KKRL; TRTRPLEQKLISEEDLAANDILDYKDDDDKV; ; Recombinant protein using RC226200 also available, TP326200
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001128159
• ORF Size: 2496 bp
• Buffer: PBS with 0.001% Pluronic F9124
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001128159.1
• RefSeq ORF: 2499 bp
• Locus ID: 55275
• UniProt ID: Q5VIR6
• Cytogenetics: 17p13.3
• MW: 94.4 kDa