Human LEPRE1 (P3H1) (NM_022356) AAV Particle

SKU RC222535A1V

AAV ORF Particle, Serotype AAV-2, P3H1 (Myc-DDK-tagged) Human prolyl 3-hydroxylase 1 (P3H1), transcript variant 1, 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: P3H1
• Synonyms: GROS1; LEPRE1; OI8
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222535 representing NM_022356; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCTGCCTCCCAAGCCGAGG; TCGAGTCCGAGGCAGGATGGGGCATGGTGACGCCTGATCTGCTCTTCGCCGAGGGGACCGCAGCCTACGC; GCGCGGGGACTGGCCCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTCCGCGCC; CTTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGGGAGCTGGACCCCGACTGGTCCCCCA; GCCCGGCCCAGGCCTCGGGCGCCGCCGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGTCGCGC; TGCCTGCCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGATGGAGCTGGAGTTC; CGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCCTACTTCAAGATCAACAAGTTGGAGAAAGCTGTTG; CTGCAGCACACACCTTCTTCGTGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGACTATTACCA; AACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACTCAACCCCATATGCAAGAATTTCGA; CTGGGAGTGCGACTCTACTCAGAGGAACAGCCACAGGAAGCTGTGCCCCACCTAGAGGCGGCGCTGCAAG; AATACTTTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGATGGCTACAACTA; CCTTGAGTACAACGCTGACCTCTTCCAGGCCATCACAGATCATTACATCCAGGTCCTCAACTGTAAGCAG; AACTGTGTCACGGAGCTTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTCCTCCCATCGCATT; ATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGGCTGTTGAATGTGCCAAGACCTA; TCTTCTCTTCTTCCCCAATGACGAGGTGATGAACCAAAATTTGGCCTATTATGCAGCTATGCTTGGAGAA; GAACACACCAGATCCATCGGCCCCCGTGAGAGTGCCAAGGAGTACCGACAGCGAAGCCTACTGGAAAAAG; AACTGCTTTTCTTCGCTTATGATGTTTTTGGAATTCCCTTTGTGGATCCGGATTCATGGACTCCAGAAGA; AGTGATTCCCAAGAGATTGCAAGAGAAACAGAAGTCAGAACGGGAAACAGCCGTACGCATCTCCCAGGAG; ATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCAAGGAGTCACTGGATGTGAGCA; GACTGACCCGGGAAGGTGGCCCCCTGCTGTATGAAGGCATCAGTCTCACCATGAACTCCAAACTCCTGAA; TGGTTCCCAGCGGGTGGTGATGGACGGCGTAATCTCTGACCACGAGTGTCAGGAGCTGCAGAGACTGACC; AATGTGGCAGCAACCTCAGGAGATGGCTACCGGGGTCAGACCTCCCCACATACTCCCAATGAAAAGTTCT; ATGGTGTCACTGTCTTCAAAGCCCTCAAGCTGGGGCAAGAAGGCAAAGTTCCTCTGCAGAGTGCCCACCT; GTACTACAACGTGACGGAGAAGGTGCGGCGCATCATAGAGTCCTACTTCCGCCTGGATACGCCCCTCTAC; TTTTCCTACTCTCATCTGGTGTGCCGCACTGCCATCGAAGAGGTCCAGGCAGAGAGGAAGGATGATAGTC; ATCCAGTCCACGTGGACAACTGCATCCTGAATGCCGAGACCCTCGTGTGTGTCAAAGAGCCCCCAGCCTA; CACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGGGACTTCGATGGCGGAAACTTTTATTTCACT; GAACTGGATGCCAAGACCGTGACGGCAGAGGTGCAGCCTCAGTGTGGAAGAGCCGTGGGATTCTCTTCAG; GCACTGAAAACCCACATGGAGTGAAGGCTGTCACCAGGGGGCAGCGCTGTGCCATCGCCCTGTGGTTCAC; CCTGGACCCTCGACACAGCGAGCGGGACAGGGTGCAGGCAGATGACCTGGTGAAGATGCTCTTCAGCCCA; GAAGAGATGGACCTCTCCCAGGAGCAGCCCCTGGATGCCCAGCAGGGCCCCCCCGAACCTGCACAAGAGT; CTCTCTCAGGCAGTGAATCGAAGCCCAAGGATGAGCTA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222535 representing NM_022356; Red=Cloning site Green=Tags(s); ; MAVRALKLLTTLLAVVAAASQAEVESEAGWGMVTPDLLFAEGTAAYARGDWPGVVLSMERALRSRAALRA; LRLRCRTQCAADFPWELDPDWSPSPAQASGAAALRDLSFFGGLLRRAACLRRCLGPPAAHSLSEEMELEF; RKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMQQNLDYYQTMSGVKEADFKDLETQPHMQEFR; LGVRLYSEEQPQEAVPHLEAALQEYFVAYEECRALCEGPYDYDGYNYLEYNADLFQAITDHYIQVLNCKQ; NCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAVECAKTYLLFFPNDEVMNQNLAYYAAMLGE; EHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQE; IGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKLLNGSQRVVMDGVISDHECQELQRLT; NVAATSGDGYRGQTSPHTPNEKFYGVTVFKALKLGQEGKVPLQSAHLYYNVTEKVRRIIESYFRLDTPLY; FSYSHLVCRTAIEEVQAERKDDSHPVHVDNCILNAETLVCVKEPPAYTFRDYSAILYLNGDFDGGNFYFT; ELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSP; EEMDLSQEQPLDAQQGPPEPAQESLSGSESKPKDEL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_022356
• ORF Size: 2208 bp
• Buffer: PBS with 0.001% Pluronic F8523
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_022356.2
• RefSeq Size: 2993 bp
• RefSeq ORF: 2211 bp
• Locus ID: 64175
• UniProt ID: Q32P28
• Cytogenetics: 1p34.2
• MW: 83.2 kDa