Human Phospholipase A2 (PLA2G4A) (NM_024420) AAV Particle

SKU RC220972A1V

AAV ORF Particle, Serotype AAV-2, PLA2G4A (Myc-DDK-tagged)-Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: PLA2G4A
• Synonyms: cPLA2; cPLA2-alpha; GURDP; PLA2G4
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220972 representing NM_024420; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATTCCCACAAGTTTACGGTAGTGG; TGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTGACATGCTTGATACTCCAGATCCCTATGTGGA; ACTTTTTATCTCTACAACCCCTGACAGCAGGAAGAGAACAAGACATTTCAATAATGACATAAACCCTGTG; TGGAATGAGACCTTTGAATTTATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATG; CCAATTATGTCATGGATGAAACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAAA; GAAAGAAGTTCCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTGAAGTTTGCTCA; TGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTTTCAGACAACAGAGAAAAGAAC; ACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGAATAGTGAAGGATTGCATTCTGCACGTGATGT; GCCTGTGGTAGCCATATTGGGTTCAGGTGGGGGTTTCCGAGCCATGGTGGGATTCTCTGGTGTGATGAAG; GCATTATACGAATCAGGAATTCTGGATTGTGCTACCTACGTTGCTGGTCTTTCTGGCTCCACCTGGTATA; TGTCAACCTTGTATTCTCACCCTGATTTTCCAGAGAAAGGGCCAGAGGAGATTAATGAAGAACTAATGAA; AAATGTTAGCCACAATCCCCTTTTACTTCTCACACCACAGAAAGTTAAAAGATATGTTGAGTCTTTATGG; AAGAAGAAAAGCTCTGGACAACCTGTCACCTTTACTGATATCTTTGGGATGTTAATAGGAGAAACACTAA; TTCATAATAGAATGAATACTACTCTGAGCAGTTTGAAGGAAAAAGTTAATACTGCACAATGCCCTTTACC; TCTTTTCACCTGTCTTCATGTCAAACCTGACGTTTCAGAGCTGATGTTTGCAGATTGGGTTGAATTTAGT; CCATACGAAATTGGCATGGCTAAATATGGTACTTTTATGGCTCCCGACTTATTTGGAAGCAAATTTTTTA; TGGGAACAGTCGTTAAGAAGTATGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGCAGTGCCTT; TTCCATATTGTTCAACAGAGTTTTGGGCGTTTCTGGTTCACAAAGCAGAGGCTCCACAATGGAGGAAGAA; TTAGAAAATATTACCACAAAGCATATTGTGAGTAATGATAGCTCGGACAGTGATGATGAATCACACGAAC; CCAAAGGCACTGAAAATGAAGATGCTGGAAGTGACTATCAAAGTGATAATCAAGCAAGTTGGATTCATCG; TATGATAATGGCCTTGGTGAGTGATTCAGCTTTATTCAATACCAGAGAAGGACGTGCTGGGAAGGTACAC; AACTTCATGCTGGGCTTGAATCTCAATACATCTTATCCACTGTCTCCTTTGAGTGACTTTGCCACACAGG; ACTCCTTTGATGATGATGAACTGGATGCAGCTGTAGCAGATCCTGATGAATTTGAGCGAATATATGAGCC; TCTGGATGTCAAAAGTAAAAAGATTCATGTAGTGGACAGTGGGCTCACATTTAACCTGCCGTATCCCTTG; ATACTGAGACCTCAGAGAGGGGTTGATCTCATAATCTCCTTTGACTTTTCTGCAAGGCCAAGTGACTCTA; GTCCTCCGTTCAAGGAACTTCTACTTGCAGAAAAGTGGGCTAAAATGAACAAGCTCCCCTTTCCAAAGAT; TGATCCTTATGTGTTTGATCGGGAAGGGCTGAAGGAGTGCTATGTCTTTAAACCCAAGAATCCTGATATG; GAGAAAGATTGCCCAACCATCATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCTCCAG; GTGTTCCAAGGGAAACTGAGGAAGAGAAAGAAATCGCTGACTTTGATATTTTTGATGACCCAGAATCACC; ATTTTCAACCTTCAATTTTCAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCACTTCAAT; ACTCTGAACAACATTGATGTGATAAAAGAAGCCATGGTTGAAAGCATTGAATATAGAAGACAGAATCCAT; CTCGTTGCTCTGTTTCCCTTAGTAATGTTGAGGCAAGAAGATTTTTCAACAAGGAGTTTCTAAGTAAACC; CAAAGCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220972 representing NM_024420; Red=Cloning site Green=Tags(s); ; MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPV; WNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCS; CPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMK; ALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLW; KKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFS; PYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEE; LENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVH; NFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPL; ILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDM; EKDCPTIIHFVLANINFRKYKAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFN; TLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_024420
• ORF Size: 2247 bp
• Buffer: PBS with 0.001% Pluronic F8262
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_024420.1
• RefSeq Size: 2875 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5321
• UniProt ID: P47712
• Cytogenetics: 1q31.1
• MW: 85 kDa