Human DGCR8 (NM_022720) AAV Particle

SKU RC219451A1V

AAV ORF Particle, Serotype AAV-2, DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: DGCR8
• Synonyms: C22orf12; DGCRK6; Gy1; pasha
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219451 representing NM_022720; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGATGGAGAGCCGAG; CTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGGTGCAGA; GGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTCTAC; GGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCC; ACAGCCCGCGCACCGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGA; TGTAAAGATTAGCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGCA; GAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGCCCTGTCAGTGGGGACGTGCATGCTTGTCCCT; TTGGCGGGAGTGTTGGTGACGGGGTAGGCATAGGGGGTGAGAGTGCTGATAAGAAGGATGAGGAGAATGA; GCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAG; CTAGATGAAGAAGGAGCAGGCGGGTTCACGGCTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGG; CCTTGAATTTCCCCTACGAGGATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAAGGCCTTTGTGC; CCCCAAAAAGAGGCGAACAGAGGAAAAATATGGCGGAGACAGCGACCATCCGTCCGATGGAGAGACAAGT; GTGCAGCCGATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACAGAGCCGCTGC; CCGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCTACACAGAGAGTCTCGGGTGGT; CACCTGGTCCAGGCCATACTTCTTGGGAACGGGAAGCATACGGAAACACGACCCTCCTCTGAGTAGCATC; CCTTGTCTGCATTATAAGAAAATGAAGGACAACGAGGAACGGGAGCAAAGCAGTGACCTCACCCCTAGTG; GGGATGTGTCCCCCGTCAAGCCCCTGAGCCGATCTGCAGAGCTGGAGTTTCCCCTGGATGAGCCTGACTC; TATGGGTGCTGACCCGGGGCCCCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCTG; GGGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATCTCGAGGAATTTCGAAGCTACC; TGGAGAAGCGTTTTGACTTTGAGCAAGTTACTGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGGCAATT; CAATCGGGAAATGAAGCGGAAGCAGGCGGAGTCCGAGAGGCCCATCTTGCCAGCCAATCAGAAGCTCATT; ACTTTATCAGTGCAAGATGCACCCACAAAGAAAGAGTTTGTTATTAACCCCAACGGGAAATCCGAGGTCT; GCATCCTGCACGAGTACATGCAGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGAGAA; CCCAAGTGAGCCTTTTGGTGCCTCGGTGACCATTGATGGTGTGACTTACGGATCTGGAACTGCAAGCAGC; AAAAAACTTGCGAAGAATAAAGCTGCCCGAGCTACACTGGAAATCCTCATCCCTGACTTTGTTAAACAGA; CCTCTGAAGAGAAGCCCAAAGACAGTGAAGAACTCGAGTATTTTAACCACATCAGCATCGAGGACTCGCG; GGTCTACGAGCTGACCAGCAAGGCTGGGCTGTTGTCTCCATATCAGATCCTCCACGAGTGCCTTAAAAGA; AACCATGGGATGGGTGACACGTCTATCAAGTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATACG; TCATGGCGTGTGGCAAGCACACAGTGCGCGGGTGGTGTAAGAACAAGAGAGTTGGAAAGCAGTTAGCCTC; ACAGAAGATCCTTCAGCTGCTGCACCCACATGTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGT; GAGAGCAGCAAGATGGTCAAGCAGGAGACATCGGACAAGAGTGTGATTGAGCTGCAGCAGTATGCCAAGA; AGAACAAGCCCAACCTGCACATCCTCAGCAAGCTCCAAGAGGAGATGAAGAGGCTAGCTGAGGAAAGGGA; GGAGACTCGAAAGAAGCCCAAGATGTCCATTGTGGCGTCCGCCCAGCCTGGCGGTGAGCCCCTGTGCACC; GTGGACGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219451 representing NM_022720; Red=Cloning site Green=Tags(s); ; METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFY; GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGA; ERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLE; LDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETS; VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI; PCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGAL; GQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI; TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS; KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR; NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR; ESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCT; VDV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_022720
• ORF Size: 2319 bp
• Buffer: PBS with 0.001% Pluronic F7995
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_022720.5
• RefSeq Size: 4461 bp
• RefSeq ORF: 2322 bp
• Locus ID: 54487
• UniProt ID: Q8WYQ5
• Cytogenetics: 22q11.21
• MW: 85.9 kDa