Human PKC epsilon (PRKCE) (NM_005400) AAV Particle

SKU RC217702A1V

AAV ORF Particle, Serotype AAV-2, PRKCE (Myc-DDK-tagged)-Human protein kinase C, epsilon (PRKCE), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: PRKCE
• Synonyms: nPKC-epsilon; PKCE
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217702 representing NM_005400; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGGCCGTGAGCTTGAAGCCCACAGCCTGGT; CGCTGCGCCATGCGGTGGGACCCCGGCCGCAGACTTTCCTTCTCGACCCCTACATTGCCCTCAATGTGGA; CGACTCGCGCATCGGCCAAACGGCCACCAAGCAGAAGACCAACAGCCCGGCCTGGCACGACGAGTTCGTC; ACCGATGTGTGCAACGGACGCAAGATCGAGCTGGCTGTCTTTCACGATGCCCCCATAGGCTACGACGACT; TCGTGGCCAACTGCACCATCCAGTTTGAGGAGCTGCTGCAGAACGGGAGCCGCCACTTCGAGGACTGGAT; TGATCTGGAGCCAGAAGGAAGAGTGTATGTGATCATCGATCTCTCAGGGTCGTCGGGTGAAGCCCCTAAA; GACAATGAAGAGCGTGTGTTCAGGGAACGCATGCGGCCGAGGAAGCGGCAGGGGGCCGTCAGGCGCAGGG; TCCATCAGGTCAACGGCCACAAGTTCATGGCCACCTATCTTCGGCAGCCCACCTACTGCTCCCATTGCAG; AGACTTCATCTGGGGTGTCATAGGAAAGCAGGGATACCAGTGTCAAGTCTGCACCTGCGTGGTCCACAAG; CGGTGCCACGAGCTCATAATCACAAAGTGTGCTGGGTTAAAGAAGCAGGAGACCCCCGACCAGGTGGGCT; CCCAGCGGTTCAGCGTCAACATGCCCCACAAGTTCGGTATCCACAACTACAAGGTCCCTACCTTCTGCGA; TCACTGTGGGTCCCTGCTCTGGGGACTCTTGCGGCAGGGTTTGCAGTGTAAAGTCTGCAAAATGAATGTT; CACCGTCGATGTGAGACCAACGTGGCTCCCAACTGTGGAGTGGATGCCAGAGGAATCGCCAAAGTACTGG; CCGACCTGGGCGTTACCCCAGACAAAATCACCAACAGCGGCCAGAGAAGGAAAAAGCTCATTGCTGGTGC; CGAGTCCCCGCAGCCTGCTTCTGGAAGCTCACCATCTGAGGAAGATCGATCCAAGTCAGCACCCACCTCC; CCTTGTGACCAGGAAATAAAAGAACTTGAGAACAACATTCGGAAAGCCTTGTCATTTGACAACCGAGGAG; AGGAGCACCGGGCAGCATCGTCTCCTGATGGCCAGCTGATGAGCCCCGGTGAGAATGGCGAAGTCCGGCA; AGGCCAGGCCAAGCGCCTGGGCCTGGATGAGTTCAACTTCATCAAGGTGTTGGGCAAAGGCAGCTTTGGC; AAGGTCATGTTGGCAGAACTCAAGGGCAAAGATGAAGTATATGCTGTGAAGGTCTTAAAGAAGGACGTCA; TCCTTCAGGATGATGACGTGGACTGCACAATGACAGAGAAGAGGATTTTGGCTCTGGCACGGAAACACCC; GTACCTTACCCAACTCTACTGCTGCTTCCAGACCAAGGACCGCCTCTTTTTCGTCATGGAATATGTAAAT; GGTGGAGACCTCATGTTTCAGATTCAGCGCTCCCGAAAATTCGACGAGCCTCGTTCACGGTTCTATGCTG; CAGAGGTCACATCGGCCCTCATGTTCCTCCACCAGCATGGAGTCATCTACAGGGATTTGAAACTGGACAA; CATCCTTCTGGATGCAGAAGGTCACTGCAAGCTGGCTGACTTCGGGATGTGCAAGGAAGGGATTCTGAAT; GGTGTGACGACCACCACGTTCTGTGGGACTCCTGACTACATAGCTCCTGAGATCCTGCAGGAGTTGGAGT; ATGGCCCCTCCGTGGACTGGTGGGCCCTGGGGGTGCTGATGTACGAGATGATGGCTGGACAGCCTCCCTT; TGAGGCCGACAATGAGGACGACCTATTTGAGTCCATCCTCCATGACGACGTGCTGTACCCAGTCTGGCTC; AGCAAGGAGGCTGTCAGCATCTTGAAAGCTTTCATGACGAAGAATCCCCACAAGCGCCTGGGCTGTGTGG; CATCGCAGAATGGCGAGGACGCCATCAAGCAGCACCCATTCTTCAAAGAGATTGACTGGGTGCTCCTGGA; GCAGAAGAAGATCAAGCCACCCTTCAAACCACGCATTAAAACCAAAAGAGACGTCAATAATTTTGACCAA; GACTTTACCCGGGAAGAGCCGGTACTCACCCTTGTGGACGAAGCAATTGTAAAGCAGATCAACCAGGAGG; AATTCAAAGGTTTCTCCTACTTTGGTGAAGACCTGATGCCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217702 representing NM_005400; Red=Cloning site Green=Tags(s); ; MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFV; TDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGRVYVIIDLSGSSGEAPK; DNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHK; RCHELIITKCAGLKKQETPDQVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNV; HRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLIAGAESPQPASGSSPSEEDRSKSAPTS; PCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLMSPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFG; KVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVN; GGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILN; GVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWL; SKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQ; DFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP*; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005400
• ORF Size: 2211 bp
• Buffer: PBS with 0.001% Pluronic F7727
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005400.2
• RefSeq Size: 5537 bp
• RefSeq ORF: 2214 bp
• Locus ID: 5581
• UniProt ID: Q02156
• Cytogenetics: 2p21
• MW: 83.5 kDa