Human DNA polymerase eta (POLH) (NM_006502) AAV Particle

SKU RC217354A1V

AAV ORF Particle, Serotype AAV-2, POLH (Myc-DDK-tagged)-Human polymerase (DNA directed), eta (POLH), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: POLH
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217354 representing NM_006502; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTACTGGACAGGATCGAGTGGTTGCTCTCGTGGACATGGACTGTTTTTTTGTTCAAGTGGAGCAGC; GGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAGGGTGGTGGAAT; AATTGCAGTGAGTTATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAGAAG; TTATGTCCAGATCTTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAGTACCGGG; AAGCCAGTGTTGAAGTGATGGAGATAATGTCTCGTTTTGCTGTGATTGAACGTGCCAGCATTGATGAGGC; TTACGTAGATCTGACCAGTGCTGTACAAGAGAGACTACAAAAGCTACAAGGTCAGCCTATCTCGGCAGAC; TTGTTGCCAAGCACTTACATTGAAGGGTTGCCCCAAGGCCCTACAACGGCAGAAGAGACTGTTCAGAAAG; AGGGGATGCGAAAACAAGGCTTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACCTCTCCAGA; CCTGCAGCTCACCGTGGGAGCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAGACTGGTTTT; CAGTGTTCAGCTGGAATTTCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAACAAGCCCAACC; GCCAAACCCTGGTTTCACATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGCAAAATCCGTAG; TCTTGGAGGAAAGCTAGGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGTGAACTGACCCAG; TTCACTGAATCCCAGCTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATATGCCATGTGCCGAG; GGATTGAACATGATCCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGTAAGAACTTCCCAGG; AAAAACAGCTCTTGCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCCCAGGAACTAGAGGAG; AGACTGACTAAAGACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTGAGCATTCGTGTACAAG; GAGACAAACGCCTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGATGCTCACAAGATGAGCCA; TGATGCATTTACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAATGGTCTCCTCCTCTCACA; ATGCTTTTCCTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCTACAGACATCACCAGCTTCT; TGAGCAGTGACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAAGCTAAGACCCAGGGAAGTGG; CCCAGCGGTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTCTTCCAAAAAGCTGCAGAAAGG; CAGAAAGTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAGGCTCCCATGAGCAATTCACCAT; CCAAGCCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAGCCCTTCTTTAAGCAGAAAAGTCT; GCTTCTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCCCAACAAAACCCATGGTCCAACTGT; AAAGCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTCCCTGTTTGTGAAGGGGTGTCGAAGC; TAGAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCCCACAACAGCCAAAGCATGCACGCCTC; TTCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACCCCAAATCCAAGTCTTCTAGCTGCTGAG; GACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTATGGGATATGCCAGAACACATGGACTATC; ATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCACTCTTCAAACCCCCAGGTTGTTTCTGCCGT; ATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCCTGCACTAATAAACGCCCCAGGCCTGAGGGC; ATGCAAACATTGGAATCATTTTTTAAGCCATTAACACAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217354 representing NM_006502; Red=Cloning site Green=Tags(s); ; MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKK; LCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISAD; LLPSTYIEGLPQGPTTAEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGF; QCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQ; FTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEE; RLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKNCNTSGIQTEWSPPLT; MLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVTSSEAKTQGSGPAVTATKKATTSLESFFQKAAER; QKVKEASLSSLTAPTQAPMSNSPSKPSLPFQTSQSTGTEPFFKQKSLLLKQKQLNNSSVSSPQQNPWSNC; KALPNSLPTEYPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHASSASKSVLEVTQKATPNPSLLAAE; DQVPCEKCGSLVPVWDMPEHMDYHFALELQKSFLQPHSSNPQVVSAVSHQGKRNPKSPLACTNKRPRPEG; MQTLESFFKPLTH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_006502
• ORF Size: 2139 bp
• Buffer: PBS with 0.001% Pluronic F7673
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_006502.1
• RefSeq Size: 3464 bp
• RefSeq ORF: 2142 bp
• Locus ID: 5429
• UniProt ID: Q9Y253
• Cytogenetics: 6p21.1
• MW: 78.2 kDa