Human POFUT1 (NM_015352) AAV Particle

SKU RC217185A1V

AAV ORF Particles, serotype AAV-2, POFUT1 (Myc-DDK-tagged)-Human protein O-fucosyltransferase 1 (POFUT1), transcript variant 1, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: POFUT1
• Synonyms: DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217185 representing NM_015352; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCGCCGCCGCGTGGGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCGCTCCCGGGGA; TGCCTGCGGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGCATGGGGCGCTTTGGGAACCA; GGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTGCTAAACCGTACCTTGGCTGTCCCTCCTTGG; ATTGAGTACCAGCATCACAAGCCTCCTTTCACCAACCTCCATGTGTCCTACCAGAAGTACTTCAAGCTGG; AGCCCCTCCAGGCTTACCATCGGGTCATCAGCTTGGAGGATTTCATGGAGAAGCTGGCACCCACCCACTG; GCCCCCTGAGAAGCGGGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGC; CCCATGAAGGAAGGAAACCCCTTTGGCCCATTCTGGGATCAGTTTCATGTGAGTTTCAACAAGTCGGAGC; TTTTTACAGGCATTTCCTTCAGTGCTTCCTACAGAGAACAATGGAGCCAGAGATTTTCTCCAAAGGAACA; TCCGGTGCTTGCCCTGCCAGGAGCCCCAGCCCAGTTCCCCGTCCTAGAGGAACACAGGCCACTACAGAAG; TACATGGTATGGTCAGACGAAATGGTGAAGACGGGAGAGGCCCAGATTCATGCCCACCTTGTCCGGCCCT; ATGTGGGCATTCATCTGCGCATTGGCTCTGACTGGAAGAACGCCTGTGCCATGCTGAAGGACGGGACTGC; AGGCTCGCACTTCATGGCCTCTCCGCAGTGTGTGGGCTACAGCCGCAGCACAGCGGCCCCCCTCACGATG; ACTATGTGCCTGCCTGACCTGAAGGAGATCCAGAGGGCTGTGAAGCTCTGGGTGAGGTCGCTGGATGCCC; AGTCGGTCTACGTTGCTACTGATTCCGAGAGTTATGTGCCTGAGCTCCAACAGCTCTTCAAAGGGAAGGT; GAAGGTGGTGAGCCTGAAGCCTGAGGTGGCCCAGGTCGACCTGTACATCCTCGGCCAAGCCGACCACTTT; ATTGGCAACTGTGTCTCCTCCTTCACTGCCTTTGTGAAGCGGGAGCGGGACCTCCAGGGGAGGCCGTCTT; CTTTCTTCGGCATGGACAGGCCCCCTAAGCTGCGGGACGAGTTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217185 representing NM_015352; Red=Cloning site Green=Tags(s); ; MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPW; IEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTC; PMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQK; YMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTAAPLTM; TMCLPDLKEIQRAVKLWVRSLDAQSVYVATDSESYVPELQQLFKGKVKVVSLKPEVAQVDLYILGQADHF; IGNCVSSFTAFVKRERDLQGRPSSFFGMDRPPKLRDEF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_015352
• ORF Size: 1164 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_015352.1
• RefSeq Size: 5249 bp
• RefSeq ORF: 1167 bp
• Locus ID: 23509
• UniProt ID: Q9H488
• Cytogenetics: 20q11.21
• MW: 43.96 kDa