Human Topoisomerase I (TOP1) (NM_003286) AAV Particle

SKU RC215658A1V

AAV ORF Particle, Serotype AAV-2, TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: TOP1
• Synonyms: TOPI
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215658 representing NM_003286; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTGGGGACCACCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAAC; ACAAAGATAAACACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAGGAGAAGGACCGGGAAAAGTC; CAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAGGAGAAGACCAAACACAAA; GATGGAAGCTCAGAAAAGCATAAAGACAAACATAAAGACAGAGACAAGGAAAAACGAAAAGAGGAAAAGG; TTCGAGCCTCTGGGGATGCAAAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACAAATTAA; AGATGAACCTGAAGATGATGGCTATTTTGTTCCTCCTAAAGAGGATATAAAGCCATTAAAGAGACCTCGA; GATGAGGATGATGCTGATTATAAACCTAAGAAAATTAAAACAGAAGATACCAAGAAGGAGAAGAAAAGAA; AACTAGAAGAAGAAGAGGATGGTAAATTGAAAAAACCCAAGAATAAAGATAAAGATAAAAAAGTTCCTGA; GCCAGATAACAAGAAAAAGAAGCCGAAGAAAGAAGAGGAACAGAAGTGGAAATGGTGGGAAGAAGAGCGC; TATCCTGAAGGCATCAAGTGGAAATTCCTAGAACATAAAGGTCCAGTATTTGCCCCACCATATGAGCCTC; TTCCAGAGAATGTCAAGTTTTATTATGATGGTAAAGTCATGAAGCTGAGCCCCAAAGCAGAGGAAGTAGC; TACGTTCTTTGCAAAAATGCTCGACCATGAATATACTACCAAGGAAATATTTAGGAAAAATTTCTTTAAA; GACTGGAGAAAGGAAATGACTAATGAAGAGAAGAATATTATCACCAACCTAAGCAAATGTGATTTTACCC; AGATGAGCCAGTATTTCAAAGCCCAGACGGAAGCTCGGAAACAGATGAGCAAGGAAGAGAAACTGAAAAT; CAAAGAGGAGAATGAAAAATTACTGAAAGAATATGGATTCTGTATTATGGATAACCACAAAGAGAGGATT; GCTAACTTCAAGATAGAGCCTCCTGGACTTTTCCGTGGCCGCGGCAACCACCCCAAGATGGGCATGCTGA; AGAGACGAATCATGCCCGAGGATATAATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCCTCCTCC; AGGACATAAGTGGAAAGAAGTCCGGCATGATAACAAGGTTACTTGGCTGGTTTCCTGGACAGAGAACATC; CAAGGTTCCATTAAATACATCATGCTTAACCCTAGTTCACGAATCAAGGGTGAGAAGGACTGGCAGAAAT; ACGAGACTGCTCGGCGGCTGAAAAAATGTGTGGACAAGATCCGGAACCAGTATCGAGAAGACTGGAAGTC; CAAAGAGATGAAAGTCCGGCAGAGAGCTGTAGCCCTGTACTTCATCGACAAGCTTGCTCTGAGAGCAGGC; AATGAAAAGGAGGAAGGAGAAACAGCGGACACTGTGGGCTGCTGCTCACTTCGTGTGGAGCACATCAATC; TACACCCAGAGTTGGATGGTCAGGAATATGTGGTAGAGTTTGACTTCCTCGGGAAGGACTCCATCAGATA; CTATAACAAGGTCCCTGTTGAGAAACGAGTTTTTAAGAACCTACAACTATTTATGGAGAACAAGCAGCCC; GAGGATGATCTTTTTGATAGACTCAATACTGGTATTCTGAATAAGCATCTTCAGGATCTCATGGAGGGCT; TGACAGCCAAGGTATTCCGTACATACAATGCCTCCATCACGCTACAGCAGCAGCTAAAAGAACTGACAGC; CCCGGATGAGAACATCCCAGCGAAGATCCTTTCTTATAACCGTGCCAATCGAGCTGTTGCAATTCTTTGT; AACCATCAGAGGGCACCACCAAAAACTTTTGAGAAGTCTATGATGAACTTGCAAACTAAGATTGATGCCA; AGAAGGAACAGCTAGCAGATGCCCGGAGAGACCTGAAAAGTGCTAAGGCTGATGCCAAGGTCATGAAGGA; TGCAAAGACGAAGAAGGTAGTAGAGTCAAAGAAGAAGGCTGTTCAGAGACTGGAGGAACAGTTGATGAAG; CTGGAAGTTCAAGCCACAGACCGAGAGGAAAATAAACAGATTGCCCTGGGAACCTCCAAACTCAATTATC; TGGACCCTAGGATCACAGTGGCTTGGTGCAAGAAGTGGGGTGTCCCAATTGAGAAGATTTACAACAAAAC; CCAGCGGGAGAAGTTTGCCTGGGCCATTGACATGGCTGATGAAGACTATGAGTTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215658 representing NM_003286; Red=Cloning site Green=Tags(s); ; MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHK; DGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPR; DEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEER; YPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFK; DWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI; ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENI; QGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAG; NEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQP; EDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILC; NHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK; LEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_003286
• ORF Size: 2295 bp
• Buffer: PBS with 0.001% Pluronic F7416
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_003286.2
• RefSeq Size: 3734 bp
• RefSeq ORF: 2298 bp
• Locus ID: 7150
• UniProt ID: P11387
• Cytogenetics: 20q12
• MW: 90.5 kDa