Human DHX35 (NM_021931) AAV Particle

SKU RC214668A1V

AAV ORF Particle, Serotype AAV-2, DHX35 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 35 (DHX35), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: DHX35
• Synonyms: C20orf15; DDX35; KAIA0875
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214668 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCGCCCGTGGGACCGGTGAAGTTCTGGCGACCCGGTACAGAGGGGCCAGGTGTAAGCATCTCTG; AAGAGAGACAAAGTCTGGCTGAAAACTCTGGGACAACGGTTGTTTACAACCCTTATGCTGCCCTTTCCAT; AGAGCAGCAGAGGCAGAAGCTGCCGGTATTCAAGCTTAGGAATCATATTTTATACTTGATAGAAAATTAT; CAGACAGTGGTGATTGTTGGTGAAACAGGATGTGGGAAGAGCACACAGATTCCTCAGTACCTTGCAGAAG; CCGGCTGGACAGCTGAAGGAAGAGTGGTAGGAGTGACCCAGCCTCGAAGAGTGGCTGCTGTTACAGTTGC; AGGGAGAGTAGCTGAAGAAAGGGGTGCAGTGCTGGGCCACGAGGTGGGCTACTGCATCCGCTTTGATGAC; TGCACCGACCAGCTGGCCACGAGAATTAAGTTTCTTACTGATGGAATGCTGGTCAGGGAAATGATGGTTG; ATCCGTTGTTAACAAAATATAGTGTCATCATGCTGGATGAAGCCCACGAGAGGACCTTGTACACTGACAT; TGCCATTGGCTTGCTAAAAAAGATTCAGAAAAAGCGAGGGGATCTTCGATTGATTGTAGCTTCAGCCACT; CTGGATGCAGACAAATTCCGGGATTTCTTTAATCAAAATGAAACCAGTGATCCAGCAAGGGATACATGTG; TGATCCTTACAGTGGAAGGGAGAACATTTCCGGTGGATATCTTTTATCTACAAAGTCCTGTTCCAGATTA; TATCAAATCAACTGTCGAAACTGTGGTGAAAATTCACCAGACAGAGGGAGACGGAGACGTTTTAGCATTT; CTTACTGGCCAGGAAGAGGTAGAAACTGTTGTGTCGATGCTCATCGAGCAGGCTCGAGCACTAGCTCGCA; CTGGGATGAAGAGACACCTCCGAGTTCTCCCCATGTATGCAGGACTGCCTTCCTTTGAGCAAATGAAAGT; GTTTGAAAGGGTGTCACGCAGTGTCAGAAAGGTGATAGTGGCCACCAATGTGGCAGAAACCTCTATCACA; ATCAGCGGCATTGTGTATGTGATCGACTGTGGCTTTGTGAAACTCCGAGCCTACAATCCCAGGACAGCTA; TTGAATGCTTGGTGGTGGTGCCAGTCTCCCAGGCATCAGCTAATCAGCGAGCAGGACGTGGTGGTCGTAG; TCGCTCGGGAAAATGTTATCGCCTTTATACAGAGGAAGCCTTTGACAAGTTGCCTCAGTCTACGGTTCCT; GAGATGCAGCGTAGTAATTTGGCACCTGTCATCCTGCAGCTGAAAGCACTAGGAATTGACAATGTCCTCA; GGTTCCACTTCATGTCGCCCCCTCCAGCACAGTCGATGGTTCAAGCCTTGGAGTTACTGTATGCTCTGGG; AGGTCTGGACAAAGACTGTCGCCTAACTGAACCGCTTGGCATGAGAATTGCAGAGTTTCCTTTGAATCCC; ATGTTTGCCAAAATGCTGCTTGAATCAGGAAACTTCGGCTGTTCTCAGGAAATTCTAAGCATCGCTGCCA; TGATGCAGATCCAGAATATCTTTGTGGTCCCCCCAAACCAGAAGTCTCACGCAATTCGAGTGCACCGTAA; ATTTGCTGTGGAGGAGGGCGACCACCTCACTATGCTCAATATATATGAAGCATTTATCAAACACAATAAG; GACTCTAAATGGTGTCAGGAACATTTCCTGAATTACAAGGGTCTTGTCAGAGCTGCGACTGTAAGAGAAC; AATTGAAAAAGCTTCTTGTCAAGTTTCAAGTGCCCAGGAAGTCTAGTGAAGGTGACCCGGATCTGGTTCT; GAGGTGCATTGTCTCCGGCTTCTTCGCCAATGCAGCGAGGTTTCATTCTACTGGAGCTTATAGGACCATC; CGTGATGACCATGAGCTGCACATACACCCTGCGTCAGTCCTCTATGCAGAGAAGCCGCCTCGCTGGGTCA; TCTATAACGAAGTTATACAGACCTCCAAGTACTACATGAGAGATGTGACTGCCATTGAATCGGCCTGGCT; GTTGGAGCTGGCTCCACACTTTTATCAACAAGGAACGCACCTGTCTCTGAAAGCCAAAAGGGCCAAGGTC; CAGGACCCG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214668 protein sequence; Red=Cloning site Green=Tags(s); ; MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQRQKLPVFKLRNHILYLIENY; QTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDD; CTDQLATRIKFLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASAT; LDADKFRDFFNQNETSDPARDTCVILTVEGRTFPVDIFYLQSPVPDYIKSTVETVVKIHQTEGDGDVLAF; LTGQEEVETVVSMLIEQARALARTGMKRHLRVLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSIT; ISGIVYVIDCGFVKLRAYNPRTAIECLVVVPVSQASANQRAGRGGRSRSGKCYRLYTEEAFDKLPQSTVP; EMQRSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNP; MFAKMLLESGNFGCSQEILSIAAMMQIQNIFVVPPNQKSHAIRVHRKFAVEEGDHLTMLNIYEAFIKHNK; DSKWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPRKSSEGDPDLVLRCIVSGFFANAARFHSTGAYRTI; RDDHELHIHPASVLYAEKPPRWVIYNEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRAKV; QDP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_021931
• ORF Size: 2109 bp
• Buffer: PBS with 0.001% Pluronic F7251
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_021931.2
• RefSeq Size: 3336 bp
• RefSeq ORF: 2112 bp
• Locus ID: 60625
• UniProt ID: Q9H5Z1
• Cytogenetics: 20q11.23-q12
• MW: 78.9 kDa