Human DGKG (NM_001080745) AAV Particle

SKU RC214133A1V

AAV ORF Particle, Serotype AAV-2, DGKG (Myc-DDK-tagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 3, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: DGKG
• Synonyms: DAGK3; DGK-GAMMA
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214133 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGTGAAGAACGGTGGGTCTCCCTCACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATT; CCTCCAAGAAGATAAAAGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCAAACAATATGACCCACA; TGAGCCGATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTAGAGGTGGACCTTCCCCAGCCA; CTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGACCACCCGACGGAGG; GAGCCAGCAACAGTGAGGCCAACAGCGCAGATACTAATATACAGAATGCAGATAATGCCACCAAAGCAGA; CGAGGCCTGTGCCCCTGATACTGAATCAAATATGGCTGAGAAGCAAGCACCAGCTGAAGACCAAGTGGCT; GCGAGCCCCCTGGAACCCCCCGTCCCTCGGTCTTCAAGCTCGGAATCCCCAGTGGTGTACCTGAAGGATG; TTGTGTGCTACCTGTCCCTGCTGGAGACGGGGAGGCCTCAGGATAAGCTGGAGTTCATGTTTCGCCTCTA; TGATTCAGATGAGAACGGTCTCCTGGACCAAGCGGAGATGGATTGCATTGTCAACCAAATGCTGCATATT; GCCCAGTACCTGGAGTGGGATCCCACAGAGCTGAGGCCTATATTGAAGGAGATGCTGCAAGGGATGGACT; ACGACCGGGACGGCTTTGTGTCTCTACAGGAATGGGTCCATGGAGGGATGACCACCATCCCATTGCTGGT; CCTCCTGGGGATGGATGACTCTGGCTCCAAGGGGGATGGGCGGCACGCCTGGACCATGAAGCACTTCAAG; AAACCAACCTACTGCAACTTCTGCCATATCATGCTCATGGGCGTCCGCAAGCAAGGCCTGTGCTGCACTT; ACTGTAAATACACTGTCCACGAACGCTGTGTGTCCAAAAACATTCCTGGTTGTGTCAAAACGTACTCAAA; AGCCAAAAGGAGTGGTGAGTTTCACCGCAAATGTGAATTATCAACGTTGTGTGACGGTGGGGAACTCAGA; GACCACATCTTACTGCCCACCTCCATATGCCCCATCACCCGGGACAGGCCAGGTGAGAAGTCTGATGGCT; GCGTGTCCGCCAAGGGCGAACTTGTCATGCAGTATAAGATCATCCCCACCCCGGGTACCCACCCCCTGCT; GGTCTTGGTGAACCCCAAGAGTGGAGGGAGACAAGGAGAAAGAATTCTTCGGAAATTCCACTATCTGCTC; AACCCCAAACAAGTTTTCAACCTGGACAATGGGGGGCCTACTCCAGGGTTGAACTTTTTCCGTGATACTC; CAGACTTCCGTGTTTTGGCCTGTGGTGGAGATGGGACAGTTGGCTGGATTTTGGATTGCATTGATAAGGC; CAACTTTGCAAAGCATCCACCAGTGGCTGTCCTGCCTCTTGGAACAGGAAATGACCTTGCCCGTTGTCTC; CGCTGGGGAGGAGGTTATGAAGGGGGCAGCTTGACAAAAATCCTGAAAGACATTGAGCAGAGCCCCTTGG; TGATGCTGGACCGCTGGCATCTGGAAGTCATCCCCAGAGAGGAAGTGGAAAACGGGGACCAGGTCCCATA; CAGCATCATGAACAACTATTTCTCCATTGGTGTGGACGCTTCCATTGCACACAGATTCCATGTGATGAGA; GAGAAACATCCTGAAAAATTCAACAGCAGGATGAAGAACAAGCTGTGGTACTTTGAATTTGGCACCTCGG; AGACTTTTGCAGCGACCTGCAAGAAACTCCACGACCACATTGAGTTGGAGTGTGATGGGGTTGGGGTGGA; CCTGAGCAACATCTTCCTGGAAGGCATTGCCATTCTCAACATTCCCAGCATGTACGGAGGCACCAATCTC; TGGGGAGAAAACAAGAAGAACCGGGCTGTGATCCGGGAAAGCAGGAAGGGTGTCACTGACCCCAAAGAAC; TGAAATTCTGCGTTCAAGACCTCAGTGACCAGCTCCTTGAAGTGGTGGGGCTAGAAGGAGCCATGGAGAT; GGGGCAGATCTACACCGGCCTGAAGAGTGCAGGCAGGAGGCTGGCCCAGTGCGCCTCTGTCACCATCAGG; ACAAACAAGCTGCTGCCAATGCAAGTGGATGGAGAACCCTGGATGCAGCCATGTTGCACGATTAAAATTA; CTCACAAGAACCAAGCGCCCATGATGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGGAA; GAGCCGTTCAAAAGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214133 protein sequence; Red=Cloning site Green=Tags(s); ; MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQP; LSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVA; ASPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHI; AQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFK; KPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSKNIPGCVKTYSKAKRSGEFHRKCELSTLCDGGELR; DHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLL; NPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCL; RWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMR; EKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNL; WGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIR; TNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001080745
• ORF Size: 2256 bp
• Buffer: PBS with 0.001% Pluronic F7157
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001080745.1, NP_001074214.1
• RefSeq Size: 5701 bp
• RefSeq ORF: 2259 bp
• Locus ID: 1608
• UniProt ID: P49619
• Cytogenetics: 3q27.2-q27.3
• MW: 84.5 kDa