Human CMG1 (IFT74) (NM_001099222) AAV Particle

SKU RC212777A1V

AAV ORF Particle, Serotype AAV-2, IFT74 (Myc-DDK-tagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 2, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: IFT74
• Synonyms: CCDC2; CMG-1; CMG1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212777 representing NM_001099222; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCAGCAATCACAAATCTTCAGCAGCTCGCCCTGTTTCAAGAGGTGGAGTTGGGTTAACAGGAAGGC; CTCCTTCTGGGATACGACCCCTATCAGGAAATATTCGAGTGGCAACTGCAATGCCACCTGGGACAGCAAG; ACCAGGTTCTCGTGGTTGTCCCATAGGGACTGGTGGAGTTCTGTCTTCTCAAATCAAAGTTGCCCATCGC; CCTGTAACACAACAAGGTTTGACTGGAATGAAAACTGGGACGAAAGGTCCCCAGAGGCAAATTTTAGACA; AATCTTACTATCTTGGGCTTCTTAGAAGTAAAATAAGTGAACTTACAACTGAAGTTAATAAACTTCAGAA; GGGAATAGAAATGTACAATCAAGAGAATTCAGTATATTTGTCATATGAAAAGAGGGCTGAGACTTTAGCT; GTTGAGATAAAAGAGCTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAACA; CTGAAATGGAAGAAGTAATGAATGATTACAATATGCTTAAAGCTCAAAATGATCGAGAAACACAAAGTTT; GGATGTCATATTTACTGAAAGACAAGCGAAAGAAAAACAAATCAGAAGTGTCGAAGAAGAAATTGAACAG; GAAAAACAAGCAACAGATGACATTATCAAAAATATGTCTTTTGAAAACCAAGTCAAGTACCTAGAGATGA; AAACCACAAATGAGAAACTGTTACAGGAATTAGATACACTTCAACAACAATTGGATTCACAGAACATGAA; AAAAGAGAGCCTGGAAGCAGAAATAGCTCACTCCCAGGTGAAACAGGAGGCGGTATTGCTGCATGAAAAA; CTTTATGAGTTGGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATAGGATCTCCAATGGAAG; AGAGAGAGAAATTACTTAAGCAGATTAAAGATGATAATCAGGAAATAGCCAGCATGGAAAGACAGTTAAC; AGATACAAAAGAAAAGATAAATCAGTTTATTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAACAC; CAAGGTGAAATGAACCAGAAATACAAGGAGCTAAAGAAAAGGGAGGAACATATGGACACTTTTATTGAGA; CTTTTGAGGAAACAAAGAATCAGGAACTGAAACGAAAGGCACAGATAGAAGCCAACATTGTTGCACTCTT; GGAGCACTGCAGTCGAAATATAAATCGTATAGAACAGATATCCTCTATCACCAATCAAGAGCTAAAGATG; ATGCAGGATGACCTCAATTTTAAATCTACTGAAGTGCAGAAATCACAAAGTACAGCTCAGAATTTGACTT; CAGACATTCAACGTCTGCAGTTGGATCTGCAGAAAATGGAGCTTCTAGAAAGTAAGATGACTGAAGAACA; GCATTCTCTAAAAAGCAAAATTAAGCAAATGACAACTGATCTGGAGATATATAATGATTTGCCAGCTTTA; AAATCATCAGGTGAAGAAAAGATAAAGAAATTACATCAGGAGAGAATGATATTATCAACCCACAGAAATG; CCTTTAAGAAAATAATGGAGAAGCAAAACATAGAGTATGAGGCACTAAAAACACAATTGCAAGAAAATGA; GACACATTCTCAGCTTACAAATTTGGAGAGAAAGTGGCAACACCTTGAGCAAAATAATTTTGCGATGAAA; GAATTCATAGCAACCAAGAGTCAAGAGAGTGATTACCAGCCAATTAAGAAAAATGTGACCAAGCAGATTG; CAGAGTACAATAAAACCATCGTGGATGCTTTACATAGCACCAGCGGAAAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212777 representing NM_001099222; Red=Cloning site Green=Tags(s); ; MASNHKSSAARPVSRGGVGLTGRPPSGIRPLSGNIRVATAMPPGTARPGSRGCPIGTGGVLSSQIKVAHR; PVTQQGLTGMKTGTKGPQRQILDKSYYLGLLRSKISELTTEVNKLQKGIEMYNQENSVYLSYEKRAETLA; VEIKELQGQLADYNMLVDKLNTNTEMEEVMNDYNMLKAQNDRETQSLDVIFTERQAKEKQIRSVEEEIEQ; EKQATDDIIKNMSFENQVKYLEMKTTNEKLLQELDTLQQQLDSQNMKKESLEAEIAHSQVKQEAVLLHEK; LYELESHRDQMIAEDKSIGSPMEEREKLLKQIKDDNQEIASMERQLTDTKEKINQFIEEIRQLDMDLEEH; QGEMNQKYKELKKREEHMDTFIETFEETKNQELKRKAQIEANIVALLEHCSRNINRIEQISSITNQELKM; MQDDLNFKSTEVQKSQSTAQNLTSDIQRLQLDLQKMELLESKMTEEQHSLKSKIKQMTTDLEIYNDLPAL; KSSGEEKIKKLHQERMILSTHRNAFKKIMEKQNIEYEALKTQLQENETHSQLTNLERKWQHLEQNNFAMK; EFIATKSQESDYQPIKKNVTKQIAEYNKTIVDALHSTSGN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001099222
• ORF Size: 1800 bp
• Buffer: PBS with 0.001% Pluronic F6937
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001099222.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 1803 bp
• Locus ID: 80173
• UniProt ID: Q96LB3
• Cytogenetics: 9p21.2
• MW: 69.1 kDa