Human MID1 (NM_000381) AAV Particle

SKU RC208776A1V

AAV ORF Particle, Serotype AAV-2, MID1 (Myc-DDK-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: MID1
• Synonyms: BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208776 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGC; CCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTC; TGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTA; GACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGC; CCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGT; CCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC; TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC; CAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG; TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA; GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGA; ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACG; TCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT; GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAAC; AGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCG; TTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATT; CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAG; AATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGA; CACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA; TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCA; AGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAA; CCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCC; AAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCA; AGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGA; TAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCTTACAAA; TCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGCCGCTGCAACAATAACT; GGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCCCCCCACCTCCGGCGCGTGGGCATCCT; GCTGGACTATGATAACGGCTCTATCGCCTTTTATGATGCTTTGAACTCCATCCACCTCTACACCTTCGAC; GTCGCATTTGCGCAGCCTGTTTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGC; TCCCTATCCCAGACCATTTGGACTGCACAGAGCAGCTGCCG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208776 protein sequence; Red=Cloning site Green=Tags(s); ; METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGL; DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVS; YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVA; ALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQII; GTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLI; PEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTI; FTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDP; KSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYK; SAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFD; VAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_000381
• ORF Size: 2001 bp
• Buffer: PBS with 0.001% Pluronic F6323
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_000381.1
• RefSeq Size: 6463 bp
• RefSeq ORF: 2004 bp
• Locus ID: 4281
• UniProt ID: O15344
• Cytogenetics: Xp22.2
• MW: 75.3 kDa