Human AFAP (AFAP1) (NM_021638) AAV Particle

SKU RC207421A1V

AAV ORF Particle, Serotype AAV-2, AFAP1 (Myc-DDK-tagged)-Human actin filament associated protein 1 (AFAP1), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: AFAP1
• Synonyms: actin filament-associated protein, 110 kDa; actin filament associated protein 1; AFAP; AFAP, AFAP-110; AFAP-110; FLJ56849; OTTHUMP00000155170
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207421 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTG; TCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAA; GGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATC; CCTCAGCCCTGGCTGCCTCCTGACAGTGGGCCTCCACCATTGCCAACATCCTCCCTCCCAGAAGGTTATT; ATGAGGAAGCTGTGCCACTGAGCCCCGGAAAAGCTCCGGAATACATCACATCAAATTATGATTCCGATGC; GATGAGCAGCTCTTATGAGTCGTATGATGAAGAGGAGGAGGATGGGAAGGGGAAGAAAACCCGGCACCAG; TGGCCCTCCGAGGAGGCCTCCATGGACCTGGTCAAGGACGCCAAAATCTGCGCCTTCCTGCTGCGGAAGA; AGCGGTTCGGCCAGTGGACCAAGTTGCTCTGCGTCATCAAAGACACCAAACTGCTGTGCTATAAAAGTTC; CAAGGACCAGCAGCCTCAGATGGAACTGCCACTCCAAGGCTGTAACATTACGTACATCCCGAAAGACAGC; AAAAAGAAGAAGCACGAGCTGAAGATTACTCAGCAGGGCACGGACCCGCTTGTTCTCGCCGTCCAGAGCA; AGGAACAGGCCGAGCAGTGGCTGAAGGTGATCAAAGAAGCCTACAGTGGTTGTAGTGGCCCCGTGGATTC; AGAGTGTCCTCCTCCACCAAGCTCCCCGGTGCACAAGGCAGAACTGGAGAAGAAACTGTCTTCAGAGAGA; CCCAGCTCAGATGGGGAGGGTGTTGTGGAAAATGGAATTACCACATGTAATGGAAAGGAGCAAGTGAAGA; GGAAGAAAAGTTCCAAATCAGAGGCCAAGGGCACTGTGTCGAAAGTCACTGGGAAAAAAATCACCAAGAT; CATCAGTCTGGGAAAGAAAAAGCCGTCCACAGACGAGCAGACCTCCTCAGCTGAGGAAGATGTTCCCACC; TGCGGCTATCTGAACGTGCTCTCCAACAGCCGCTGGCGAGAGCGCTGGTGCCGAGTGAAAGATAACAAGC; TCATTTTCCACAAGGACAGGACCGACCTGAAGACCCATATTGTGTCTATTCCGCTCCGTGGCTGCGAGGT; GATCCCGGGTTTGGATTGTAAACATCCTCTGACGTTCCGGCTGCTGCGCAACGGCCAGGAGGTTGCAGTA; TTGGAGGCATCTTCTTCTGAAGACATGGGCAGGTGGATTGGGATTTTACTCGCAGAGACGGGATCGTCCA; CAGACCCGGAGGCTCTGCACTATGACTACATTGATGTGGAGATGTCTGCAAGTGTCATTCAGACAGCCAA; ACAGACCTTCTGTTTCATGAACAGGCGTGTTATATCTGCTAACCCATATCTAGGGGGCACCTCCAACGGC; TATGCCCACCCCAGCGGGACGGCACTTCATTATGACGATGTCCCGTGCATCAACGGCTCGCTCAAGGGTA; AAAAGCCCCCCGTGGCGTCTAATGGGGTCACAGGAAAAGGGAAGACTCTGAGCAGTCAGCCAAAGAAAGC; GGATCCCGCGGCTGTTGTGAAAAGGACGGGTTCGAATGCTGCCCAGTACAAGTATGGCAAGAACCGGGTA; GAAGCAGATGCCAAGCGGCTACAGACCAAAGAGGAGGAGCTGCTGAAGAGGAAAGAGGCCCTGCGGAATA; GGCTGGCCCAGCTCCGCAAGGAAAGAAAAGACCTTCGAGCGGCTATTGAAGTGAACGCCGGCAGGAAGCC; GCAGGCGATCCTGGAGGAGAAGCTGAAGCAGCTGGAGGAGGAGTGCCGGCAGAAGGAGGCGGAGCGTGTC; AGCCTGGAGCTGGAGCTGACGGAGGTCAAGGAGAGCCTGAAGAAAGCGCTGGCGGGCGGAGTCACCCTGG; GGCTGGCCATCGAGCCCAAGTCAGGGACATCGAGTCCACAGTCTCCAGTGTTCCGGCACCGGACCCTGGA; AAACTCGCCCATCTCCAGCTGTGACACCAGTGACACCGAGGGCCCCGTGCCGGTGAACAGCGCGGCCGTC; TTGAAGAAGAGCCAGGCTGCCCCGGGCAGCTCCCCCTGCCGAGGGCATGTGCTGCGGAAGGCCAAGGAAT; GGGAATTGAAGAACGGGACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207421 protein sequence; Red=Cloning site Green=Tags(s); ; MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEI; PQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQ; WPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDS; KKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSER; PSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPT; CGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAV; LEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNG; YAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRV; EADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERV; SLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAV; LKKSQAAPGSSPCRGHVLRKAKEWELKNGT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_021638
• ORF Size: 2190 bp
• Buffer: PBS with 0.001% Pluronic F6063
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_021638.4, NP_067651.2
• RefSeq Size: 7479 bp
• RefSeq ORF: 2192 bp
• Locus ID: 60312
• Cytogenetics: 4p16.1
• MW: 80.7 kDa