Human SPG7 (NM_003119) AAV Particle

SKU RC206340A1V

AAV ORF Particle, Serotype AAV-2, SPG7 (Myc-DDK-tagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SPG7
• Synonyms: CAR; CMAR; PGN; SPG5C
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206340 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCGTGCTGCTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGG; GCCCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATGGCCAGCAG; GCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAATTGAGACTGCTAACCCCT; ACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTAGTTCAGAATCCAGTCAGACTCTGGCAAC; TTTTAGGTGGTACTTTCTATTTTAACACCTCAAGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAA; GGGGAAGGCGCCTGAAGAGGACGAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAG; CGGCTGCGCACCTTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCA; GCATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAGGTGGTGCC; TGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCCGTGGTGTTTGGGCGGCCTCGGCTAGCCTTG; ATGTACCGAATGCAGGTTGCAAATATTGACAAGTTTGAAGAGAAGCTTCGAGCAGCTGAAGATGAGCTGA; ATATCGAGGCCAAGGACAGGATCCCAGTTTCCTACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTC; TGTGGGGATGACGGCAGTGGGCCTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGG; GAAGGTGGATTCAGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGGAAGATGGGGA; AAGGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAAGTCCGCGAGTTTGTGGATTA; TCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAGGTCCCAAAGGGCGCACTGCTGCTCGGCCCC; CCCGGCTGTGGGAAGACGCTGCTGGCCAAGGCGGTGGCCACGGAGGCTCAGGTGCCCTTCCTGGCGATGG; CCGGCGCAGAGTTCGTGGAGGTCATTGGAGGCCTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGC; CCGAGCCCGGGCCCCCTGCATCGTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACC; ATGTCCGGCTTCTCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATGGATGGAATGG; GTACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATTTTGGACGGTGCTCTGATGAG; GCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCCACGCTGCAGGAGAGGCGGGAGATTTTTGAG; CAGCACCTGAAGAGCCTGAAGCTGACCCAGTCCAGCACCTTTTACTCCCAGCGTCTGGCAGAGCTGACAC; CAGGATTCAGTGGGGCTGACATCGCCAACATCTGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACA; CACTTCCGTGCACACTCTCAACTTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGT; AAGATCCTGTCCAAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTGGTGGGCTGGA; TGCTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACAAACGCCGCCCTGGGCTTTGC; TCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAGCAGCTGTTTGAGCGGATGTGCATGGCCCTG; GGAGGACGGGCCTCGGAAGCACTGTCCTTCAACGAGGTCACTTCTGGGGCACAGGACGACCTGAGGAAGG; TCACCCGCATCGCCTACTCCATGGTGAAGCAGTTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCC; TGAGGCGCAGGAGGGCCTCATGGGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGAC; CATGAAGCAAGACTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGACAACCTGGACA; AGTTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAGGCTCTCAT; TGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCGCAGAGGTGGATCGACGCCCAGAGGGAGAAA; CAGGACTTGGGCGAGGAGGAGACCGAAGAGACCCAGCAGCCTCCACTTGGAGGCGAAGAGCCGACTTGGC; CCAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206340 protein sequence; Red=Cloning site Green=Tags(s); ; MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTP; TFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRE; RLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLAL; MYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGR; EGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGP; PGCGKTLLAKAVATEAQVPFLAMAGAEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTT; MSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADILDGALMRPGRLDRHVFIDLPTLQERREIFE; QHLKSLKLTQSSTFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTLNFEYAVERVLAGTAKKS; KILSKEEQKVVAFHESGHALVGWMLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTKEQLFERMCMAL; GGRASEALSFNEVTSGAQDDLRKVTRIAYSMVKQFGMAPGIGPISFPEAQEGLMGIGRRPFSQGLQQMMD; HEARLLVAKAYRHTEKVLQDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQRWIDAQREK; QDLGEEETEETQQPPLGGEEPTWPK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_003119
• ORF Size: 2385 bp
• Buffer: PBS with 0.001% Pluronic F5868
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_003119.2
• RefSeq Size: 3102 bp
• RefSeq ORF: 2388 bp
• Locus ID: 6687
• UniProt ID: Q9UQ90
• Cytogenetics: 16q24.3
• MW: 88.2 kDa